Báo cáo khoa học: The activity of Plasmodium falciparum arginase is mediated by a novel inter-monomer salt-bridge between Glu295–Arg404 doc

... enzymes: a promising approach to therapy of African sleeping sickness, Chagas’ disease, and leishmaniasis. Amino Acids 33, 359–366. 12 Wallace HM (2007) Targeting polyamine metabolism: a viable therapeutic ... templates. P. falciparum Glu295 aligns with an Asp in mam- mals (human arginase II: Asp223; rat arginase I: Asp204), fungi and bacteria (B. caldovelox arginase: Asp199). I...

Ngày tải lên: 29/03/2014, 23:20

... identified. Although calpain 7 contains a C2-like domain, it lacks a penta-EF-hand domain and is classified as an atypical calpain. As one of the significant structural features, mammalian calpain 7 ... were analyzed by WB analysis with mAb against GFP or polyclonal antibody (pAb) against calpain 7 (raised against recombinant MIT domains [28]). In the case of WB analysis with mAb...

Ngày tải lên: 18/02/2014, 04:20

... 5¢-GAACC AGGTTTTCCGGCCCA-3¢ for DHFR; 5¢-GTGCCAAT GGCTGGCAGATCA-3¢ and 5¢-ACCATCCTGCTGCA CTTGGGC-3¢ for Sp1; 5¢-CTCCACACACTCCAGTT AGGA-3¢ and 5¢-CTGATTTAAGCATGGATTCCA-3¢ for Rb; and 5¢-CGCAGTTTCCCCGACTTCCC-3¢ ... cells by a mechanism involving transcriptional activation and, also in the case of Rb, by an increase in its stability. Materials and methods Materials UCN-01 was kindly pr...

Ngày tải lên: 16/03/2014, 23:20

... GAACTCCAGGAAAAACAAGTCAAG TTTTGACAAGTCCAAATACCTCTTT CGGCCAACGTTAAAAAGTATCGGATGGAATTTTGGCCG PfGPx (PFL0595C) AATTGTGATTCGATGCATGATG TTTATCGACGAGAAATTTTCCAA CGGCCAACGTTAAAAAGTATCGGATGGAATTTTGGCCG Transient ... (M19172) TGACTACGTCCCTGCCCTT ACAATTCATCATATCTTTCAATCGG GGGGGACACCGCCCGTCGCTCCCCC PfGR (NC_004317) AGTGGAGGAATGGCTGCAG CCTAAACGGGATTTTTCGACA CGGGCAGCAAGGCATAACGCAAGCCCG PfTrxR (AL929357) TT...

Ngày tải lên: 19/02/2014, 07:20

... of the task and an analysis of the users' and adviser's plans and goals. BOUNDARY MARKERS The analysis of boundary markers revealed reliable indicators at the opening of subdialogues ... on the basis of the task structure An important prerequisite in the interpretation of user-adviser dialogues is to analyze the task the users are trying to per...

Ngày tải lên: 21/02/2014, 20:20

... reverse transcriptase PCR was also performed to isolate the cDNA encoding ICDH. Using the specific oligonucleotides (sense 5¢-GCGCGCGGTCTCCGCGCA TGGGAAAGCATATACGAATTTTAAAAAATCAAT ACC-3¢ and antisense ... and 10–15% parasitaemia were isolated by saponin lysis according to [32]. Genomic DNA was isolated from the parasites according to Krnajski et al. 2002 [33]. Total RNA was isolated fro...

Ngày tải lên: 08/03/2014, 02:20

... 4C). As in the low-concentration range of M pH the observed activity is rather small, it is likely that either M pH is inactive and the measured activity is a reflection of the formation of tiny amounts ... known. Our interest in the enolase from the malarial para- site (Plasmodium falciparum) originates from the fact that the intraerythrocytic stages of...

Ngày tải lên: 23/03/2014, 09:20

... Forward (nt 1552–1585 PAI-2) SJS260 AACTCACCAT AGGAATGCATAATAAATAACAAAG Reverse (nt 1585–1552 PAI-2) SJS261 CTTTGTTA AAGCTTATGCATTCCTATGGTGAGTT Forward (nt 1552–1585 PAI-2) SJS262 AACTCACCAT AGGAATGCATAAGCTTTAACAAAG ... 1491–1508 PAI-2) SJS138 TACG AGATCT TAGCTACATTAAATAGGC Reverse (nt 1620–1603 PAI-2) SJS172 GGGATCATGCCCA AGCTTATTTTCCTTACT Forward (nt 1491–1520 PAI-2) SJS173 AGTAAGGAAAATA AG...

Ngày tải lên: 16/02/2014, 09:20

... were calculated from the standard errors associated with the means of the measured values (i.e. by calculating the square root of the sum of the associated individual errors squared). The maximum ... (Fig. 3A) . The presence of aB-crystallin at a 1.0 : 0.5 w ⁄ w ratio of cata- lase : aB-crystallin inhibited the precipitation of cata- lase by 71 ± 7% (Fig. 3...

Ngày tải lên: 18/02/2014, 16:20

... residues Asp38 and Glu111. In RNase-AA-GG the ‘basic sur- face’ is more similar to that of BS-RNase because of the removal of the four carboxylates. The ‘basic sur- face’ of the mutant HHP-RNase is ... is very similar to that of RNase-AA-GG as the presence of Glu111 is coun- terbalanced by basic residues not present on RNase- AA-GG. This material is availab...

Ngày tải lên: 19/02/2014, 06:20