Báo cáo khoa học: The activity of Plasmodium falciparum argi

Báo cáo khoa học: The activity of Plasmodium falciparum arginase is mediated by a novel inter-monomer salt-bridge between Glu295–Arg404 doc

... enzymes: a promising approach to therapy of African sleeping sickness, Chagas’ disease, and leishmaniasis. Amino Acids 33, 359–366. 12 Wallace HM (2007) Targeting polyamine metabolism: a viable therapeutic ... templates. P. falciparum Glu295 aligns with an Asp in mam- mals (human arginase II: Asp223; rat arginase I: Asp204), fungi and bacteria (B. caldovelox arginase: Asp199). I...

Ngày tải lên : 29/03/2014, 23:20

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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... identified. Although calpain 7 contains a C2-like domain, it lacks a penta-EF-hand domain and is classified as an atypical calpain. As one of the significant structural features, mammalian calpain 7 ... were analyzed by WB analysis with mAb against GFP or polyclonal antibody (pAb) against calpain 7 (raised against recombinant MIT domains [28]). In the case of WB analysis with mAb...

Ngày tải lên : 18/02/2014, 04:20

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Báo cáo khoa học: The expression of retinoblastoma and Sp1 is increased by low concentrations of cyclin-dependent kinase inhibitors ppt

... 5¢-GAACC AGGTTTTCCGGCCCA-3¢ for DHFR; 5¢-GTGCCAAT GGCTGGCAGATCA-3¢ and 5¢-ACCATCCTGCTGCA CTTGGGC-3¢ for Sp1; 5¢-CTCCACACACTCCAGTT AGGA-3¢ and 5¢-CTGATTTAAGCATGGATTCCA-3¢ for Rb; and 5¢-CGCAGTTTCCCCGACTTCCC-3¢ ... cells by a mechanism involving transcriptional activation and, also in the case of Rb, by an increase in its stability. Materials and methods Materials UCN-01 was kindly pr...

Ngày tải lên : 16/03/2014, 23:20

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Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx

... GAACTCCAGGAAAAACAAGTCAAG TTTTGACAAGTCCAAATACCTCTTT CGGCCAACGTTAAAAAGTATCGGATGGAATTTTGGCCG PfGPx (PFL0595C) AATTGTGATTCGATGCATGATG TTTATCGACGAGAAATTTTCCAA CGGCCAACGTTAAAAAGTATCGGATGGAATTTTGGCCG Transient ... (M19172) TGACTACGTCCCTGCCCTT ACAATTCATCATATCTTTCAATCGG GGGGGACACCGCCCGTCGCTCCCCC PfGR (NC_004317) AGTGGAGGAATGGCTGCAG CCTAAACGGGATTTTTCGACA CGGGCAGCAAGGCATAACGCAAGCCCG PfTrxR (AL929357) TT...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: "The Structure of User-Adviser Dialogues: Is there Method in their Madness?" pdf

... of the task and an analysis of the users' and adviser's plans and goals. BOUNDARY MARKERS The analysis of boundary markers revealed reliable indicators at the opening of subdialogues ... on the basis of the task structure An important prerequisite in the interpretation of user-adviser dialogues is to analyze the task the users are trying to per...

Ngày tải lên : 21/02/2014, 20:20

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Báo cáo khoa học: Isocitrate dehydrogenase of Plasmodium falciparum Energy metabolism or redox control? doc

... reverse transcriptase PCR was also performed to isolate the cDNA encoding ICDH. Using the speciﬁc oligonucleotides (sense 5¢-GCGCGCGGTCTCCGCGCA TGGGAAAGCATATACGAATTTTAAAAAATCAAT ACC-3¢ and antisense ... and 10–15% parasitaemia were isolated by saponin lysis according to [32]. Genomic DNA was isolated from the parasites according to Krnajski et al. 2002 [33]. Total RNA was isolated fro...

Ngày tải lên : 08/03/2014, 02:20

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Báo cáo khoa học: Differential susceptibility of Plasmodium falciparum versus yeast and mammalian enolases to dissociation into active monomers doc

... 4C). As in the low-concentration range of M pH the observed activity is rather small, it is likely that either M pH is inactive and the measured activity is a reﬂection of the formation of tiny amounts ... known. Our interest in the enolase from the malarial para- site (Plasmodium falciparum) originates from the fact that the intraerythrocytic stages of...

Ngày tải lên : 23/03/2014, 09:20

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Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... Forward (nt 1552–1585 PAI-2) SJS260 AACTCACCAT AGGAATGCATAATAAATAACAAAG Reverse (nt 1585–1552 PAI-2) SJS261 CTTTGTTA AAGCTTATGCATTCCTATGGTGAGTT Forward (nt 1552–1585 PAI-2) SJS262 AACTCACCAT AGGAATGCATAAGCTTTAACAAAG ... 1491–1508 PAI-2) SJS138 TACG AGATCT TAGCTACATTAAATAGGC Reverse (nt 1620–1603 PAI-2) SJS172 GGGATCATGCCCA AGCTTATTTTCCTTACT Forward (nt 1491–1520 PAI-2) SJS173 AGTAAGGAAAATA AG...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... were calculated from the standard errors associated with the means of the measured values (i.e. by calculating the square root of the sum of the associated individual errors squared). The maximum ... (Fig. 3A) . The presence of aB-crystallin at a 1.0 : 0.5 w ⁄ w ratio of cata- lase : aB-crystallin inhibited the precipitation of cata- lase by 71 ± 7% (Fig. 3...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... residues Asp38 and Glu111. In RNase-AA-GG the ‘basic sur- face’ is more similar to that of BS-RNase because of the removal of the four carboxylates. The ‘basic sur- face’ of the mutant HHP-RNase is ... is very similar to that of RNase-AA-GG as the presence of Glu111 is coun- terbalanced by basic residues not present on RNase- AA-GG. This material is availab...

Ngày tải lên : 19/02/2014, 06:20

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