Báo cáo khoa học: High-resolution crystal structures of the flavoprotein NrdI in oxidized and reduced states – an unusual flavodoxin pot

... to these as the 40s and 70s loops, respectively, in baNrdI. The flavin moiety is sandwiched between Trp74 on one face and the side chain of Thr42 and the main chain atoms of residues 4 2–4 4 on the ... P-loop. The flavin moiety of FMN binds in a pocket formed by loops at the C-terminal ends of b-strands 3 and 4 (loops 4 2–4 9 and 7 1–7 9, respectively),...

Ngày tải lên: 29/03/2014, 21:20

... [10,11], and the VanR–VanS system activates the expression of vancomycin resistance [12,13]. Phosphate control of the production of actino- rhodin and undecylprodigiosin in S. lividans and S. coelicolor ... between the purified cytoplasmic domain of SenS (SenS c ) and SenR. Using designed mutant pro- teins, the phosphorylation sites within SenS c and SenR have bee...

Ngày tải lên: 07/03/2014, 10:20

... membrane and can be easily Table 1. Activities of Hyn hydrogenase in vivo and in vitro in the presence and absence of the Isp1 and Isp2 proteins. The results are given as percentages of the level ... electron carrier) and in vitro (in the presence of redox viologen dyes). The data in Table 1 unequivocally prove that the in vivo H 2 -producing activ...

Ngày tải lên: 16/03/2014, 03:20

... talin, which binds the integrin to an actin fila- ment [16]. Stretching talin activates vinculin, a FA pro- tein, leading to reinforcement of the FA [17] (Fig. 1). Therefore, a rearrangement of the ... direction of shear stress. In (C), the force is in the direction of tension of actin filaments, maintained with the help of neighboring cells in contact. In (D),...

Ngày tải lên: 22/03/2014, 21:20

... polymorphisms in the UGT1A7 and UGT1A9 glucuronidating enzymes in Caucasian and African-American subjects and their impact on the metabolism of 7-ethyl-10-hydroxycamp- tothecin and flavopiridol anticancer ... 33 is involved in the interactions of the enzyme with the substrate in both UGT2B4 and UGT2B7. In contrast to the F33L mutation, which reduces the ac...

Ngày tải lên: 23/03/2014, 09:21

... Kallio-Ratilainen and Johanna Aura for their technical assistance and Dr Xiaoyan Wu for assistance in protein purification. The Academy of Finland and the National Technology Agency of Finland, TEKES, ... between the C-terminal Glu (or Asp) of b-strand B6 (BAX and BCX have Asp) and Arg of the loop between b-strands B7 and A6. Thermophiles tend to have more salt br...

Ngày tải lên: 20/02/2014, 23:20

... & CC( 7–8 ) steps (Fig. 2B) of the DNA strand, and GA(2 3–2 4) (Fig. 2C) and AT(2 4–2 5) (Fig. 2D) steps of the PNA strand, flanking the A24 C7 mismatch in PD wt (Scheme 1), and the corresponding AT( 6–7 ) ... destabilization and hence, diminution of the melting temperature (T m ) in the former. Base stacking in the vicinity of A C mismatch in...

Ngày tải lên: 07/03/2014, 21:20

... Asn2 and Asn15, predominantly express the structure Mana 1–6 (GlcNAc b 1–2 Mana 1–3 )Manb 1–4 GlcNAcb 1–4 GlcNAc. In the case of bovine, human and frog rhodopsin, both of these sites are glycosylated. On the ... conformational change in the polypeptide chain. Examination of the N-glycan structures provides insight into the processing of sugar chains in...

Ngày tải lên: 23/03/2014, 17:22

... obtained with the transition at posi- tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the other two ... found in the 23S rRNA variant containing the double mutation U1911C ⁄ U1915C (Fig. 3). Thus, isomerization of uri- dines 1911 and 1917 in H69 occurs autonomousl...

Ngày tải lên: 18/02/2014, 16:20

... report the binding potentials between the phenol-hydro- xyl group of bisphenol A and estrogen-related receptor c residues Glu275 and Arg316 in the ligand-binding domain. By inducing mutations in other amino ... structure BPA and its ball -and- stick structure, together with a space-filling structure in the ligand-binding pocket of the ERRc. The space-filling structur...

Ngày tải lên: 18/02/2014, 16:20