Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf

... ª 2009 FEBS Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol Jong Han Lee 1 , K. Hoa Nguyen 1 , Suresh Mishra 1,2 and B. L. Gre ´ goire ... whether PHB is expressed in b-cells and protects these cells against deleterious effects of ethanol, using INS-1E and RINm5F b-cell lines....

Ngày tải lên: 29/03/2014, 08:20

... recruitment of Sin3 and or Rpd3, rather than by regulating Sin3 ⁄ Rpd3 directly. A good candidate for a factor regulating Sin3 ⁄ Rpd3 binding is Stb1, because it binds to both Swi6 and Sin3 [20,21,32,34]. ... transcription is inactivated [33]. Phosphory- lation of Stb1 inhibits interaction between Swi6 and Stb1 [20]. Our finding of cell-cycle-specific promoter binding by Sin3 i...

Ngày tải lên: 23/03/2014, 05:22

... observed two dominant isoelectric points of 7.7 and 7.2 and two minor points at 7.4 and 7.1 (Fig. 1C). Analysis of p95 revealed isoelectric points of 7.7, 7.2 and 7.1 (Fig. 1C). These points were located ... folding of proteins or inappro- priate assembly of multimeric protein complexes can in uence the surface and overall expression of these proteins in cells and c...

Ngày tải lên: 23/03/2014, 12:20

... [13,27]. Substitution of certain amino acids in the intrarepeat loops abrogates the binding of some B subunits but not others [13,28]. The results here de®ne two distinct PP2A binding domains in the B subunits ... [25] and i maged u sing a Molecular D ynamics PhosphorImager. RESULTS AND DISCUSSION Identi®cation of two A subunit-binding domains in B56a To determine the minimal...

Ngày tải lên: 08/03/2014, 10:20

... vector pQCXIN, pQCXIN-NANOGP8 and pQCXIN-NANOGP8-GFP using Lipofectamine TM 2000 according to the manufac- turer’s instructions. Stable clones were selected and isolated in media containing 500 ... transcribed in HepG2, while Nanog was not transcribed in OS732. Thus, it is likely the protein of NANOGP8 and ⁄ or Nanog was detected in cancer line HepG2 and the protein of N...

Ngày tải lên: 07/03/2014, 12:20

... that His41 of NirF, which aligns with His200 of the d 1 heme domain of cd 1 , is essential both for this binding and for the production of d 1 heme; replacement of His41 by Ala, Cys, Lys and Met ... and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SBN13 (DnirF) c...

Ngày tải lên: 15/02/2014, 01:20

... data AcLDL 1 2 Visfatin/cyclophilin mRNAVisfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA CD36/cyclophilin mRNA FABP4/cyclophilin mRNA Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA 3 4 3 ... were abolished when insulin receptor signalling was blocked [8], in line with the report that visfatin could bind and activate insulin receptors [14]. However, the in...

Ngày tải lên: 06/03/2014, 22:21

... his3:hisG/his3::hisG trp1::hisG/trp1::hisG This study MR211 S1278b; MATa ura3–52 his3:hisG leu2::hisG ras2::kanMX MR298 S1278b; MATa/a ras2::kanMX/ras2::kanMX ura3–52/ura3–52 his3:hisG/his3:hisG leu2::hisG ... his3:hisG/his3:hisG leu2::hisG leu)2::hisG This study AR2 S1278b; MATa/a sut2::S.pombe his5 + /sut2::S.pombe his5 + ura3–52/ura3–52 his3:hisG/his3:hisG leu2::hisG/LEU2 TRP1/trp1::hisG T...

Ngày tải lên: 07/03/2014, 15:20

... these interactions. The side chain of histidine is somewhat smaller than that of tyrosine and hydrophobic interaction s of carbon atoms in the aromatic ring of tyrosine with the neighbouring residues ... abolished. This could result in loosening of th e stability of the structure in this part of the monomer. Although tyrosine-42 is distant from subunit interaction in...

Ngày tải lên: 07/03/2014, 16:20

... similar unfold- ing transitions, suggesting that the Cys side chain is unique in imparting local stability. Discussion We began this study with the intention of establishing the role of the completely ... conservation of this residue. Indeed, an earlier investigation of the C126S and C126A mutants of yeast TIM revealed that their activ- ity remained undiminished, with the muta...

Ngày tải lên: 14/03/2014, 23:20