Molecular Biotechnology-Lession 2: Recombinant DNA technology docx

... to vectors  Self-replicating  Double stranded  Circular DNA  Independent extrachromosomal DNA in bacteria 1kb-500kb  Origin of DNA replication, ori  High (10-100) & low copy plasmid ... pigment 5,5'-dibromo-4,4'- dichloro-indigo

Ngày tải lên: 23/03/2014, 22:20

... RNase H, DNA polymerase I, and DNA ligase. Cloning of cDNA using BamHI linkers Screening  What screening method can be used to identify a recombinant clone with a target gene in a cDNA library ... produce DNA fragments of appropriate size for constructing a genomic library. cDNA Libraries are best for eukaryotes Figure 8.8 The synthesis of double-stranded complementary DN...

Ngày tải lên: 13/03/2014, 19:33

... Activity 7 .2: Determining the Impact of Technology on a Windows DNA Design In this activity, you will identify possible implications of technology on a Microsoft ® Windows ® DNA solution. ... minute discussion. 50 Activity 7 .2: Determining the Impact of Technology on a Windows DNA Design Exercise 1: Determining Technology Implications ! Determine technology...

Ngày tải lên: 21/12/2013, 06:16

... new DNA with polymerase Cycle 3 (focus on DNA segments bounded by primers) 2 duplex molecules of desired product Recombinant DNA I Basics of molecular cloning Polymerase chain reaction cDNA ... in a population • Forensics - test whether suspect’s DNA matches DNA extracted from evidence at crime scene • Etc, etc Recombinant DNA Technology • Utilizes microbiological...

Ngày tải lên: 13/03/2014, 18:39

... reaction (PCR) mRNA DNA cDNA Reverse transcriptase Taq DNA polymerase  In RT-PCR, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme ... of RT-PCR  44 o C, 1hr  92 o C, 10min  4 o C, 0min Step 1: Making cDNA Step 2: Regular PCR Basic techniques in DNA technology  Gene cloing

Ngày tải lên: 23/03/2014, 22:20

... restriction enzymes (Roche Biosciences; Amersham Pharmacia), PWO DNA polymerase and shrimp alkaline phosphatase (Roche Bio- sciences), T4 DNA ligase (Invitrogen), T4 polynucleotide kinase (Promega), ... for substrate phage library. The BamHIandXbaI/HindIII DNA recognition sites were introduced by PCR onto 5¢ and 3¢ ends, respectively, of the cDNA encoding the CFP fluorescent protein. The...

Ngày tải lên: 31/03/2014, 23:20

... thước đo góc , giấy kẻ ô vuông cho các bài tập11,14,19. - Bảng phụ: Bảng 1: Ghi ?2 SGK ; Bảng ph 2: Ghi?3 SGK 2. Học sinh : - Thước chia khoảng, thước đo góc - Nắm vững đònh nghóa và tính chất ... 110 0 , góc N = 70 0 , góc S = 90 0 . c) Hai góc đối đỉnh của hình thang cân thì bù nhau. 12 / H 2: Tính chất hình thang cân: HĐ2.1: Đònh lí 1 GV: Vẽ hình thang cân ABCD, cho HS đo độ dài 2...

Ngày tải lên: 15/10/2013, 00:11

... WHITE PAPER Fundamentals of Ethernet Technology Fundamentals of Ethernet Technology This white paper provides a brief tutorial on Ethernet – a family of ... Important Not Important Fundamentals of Ethernet Technology DCE Packet A Packet B DCE Figure 2. Packet-Switched Network Packet-Switching: A Data-Friendly Technology Differing requirements drove the ... only data, but voice and...

Ngày tải lên: 10/12/2013, 02:15

... Architecture 9 The Web Services Programming Model 18 Review 21 Module 2: Web Service Architectures 4 Module 2: Web Service Architectures The preceding three service-oriented architecture ... robust distributed applications, there are a number of requirements that must be met. Module 2: Web Service Architectures 1 Overview ! Service-Oriented Architecture ! Web S...

Ngày tải lên: 10/12/2013, 16:15

... CASE STUDY Richland-Grant Telephone Cooperative Relies on ADC’s Hardened Connector Technology to Achieve Fast, Low-Cost Network Installation BACKGROUND Established in 1951, Richland-Grant ... lowest cost possible. To meet its delivery goals, it’s important that RGTC keep its communications technology on the cutting edge of emerging trends. Creating an efficient, scalable infrastruct...

Ngày tải lên: 10/12/2013, 18:15