Molecular Biotechnology-Lession 2: Recombinant DNA technology docx
Molecular Biotechnology-Lession 2: Recombinant DNA technology docx
... to vectors
Self-replicating
Double stranded
Circular DNA
Independent
extrachromosomal DNA in
bacteria 1kb-500kb
Origin of DNA replication, ori
High (10-100) & low copy
plasmid ... pigment 5,5'-dibromo-4,4'-
dichloro-indigo
Chapter 8: Recombinant DNA Technology
... RNase H, DNA polymerase I,
and DNA ligase.
Cloning of cDNA
using BamHI
linkers
Screening
What screening method can be used to identify a
recombinant clone with a target gene in a cDNA
library ... produce DNA
fragments of appropriate size for constructing a genomic library.
cDNA Libraries are best for eukaryotes
Figure 8.8 The synthesis of double-stranded complementary DN...
Tài liệu Activity 7.2: Determining the Impact of Technology on a Windows DNA Design docx
...
Activity 7 .2: Determining the Impact of
Technology on a Windows DNA
Design
In this activity, you will identify possible implications of technology on a
Microsoft
®
Windows
®
DNA solution. ... minute
discussion.
50 Activity 7 .2: Determining the Impact of Technology on a Windows DNA Design
Exercise 1: Determining Technology Implications
!
Determine technology...
Recombinant DNA I - Basics of molecular cloning Polymerase chain reaction cDNA clones and screening
... new DNA with polymerase
Cycle 3 (focus on DNA segments bounded by primers)
2 duplex
molecules
of desired
product
Recombinant DNA I
Basics of molecular cloning
Polymerase chain reaction
cDNA ... in a
population
•
Forensics - test whether suspect’s DNA matches
DNA extracted from evidence at crime scene
•
Etc, etc
Recombinant DNA Technology
•
Utilizes microbiological...
Molecular Biotechnology-Lession 3: Basic techniques in DNA technology ppt
... reaction (PCR)
mRNA
DNA
cDNA
Reverse transcriptase
Taq DNA polymerase
In RT-PCR, an RNA strand is first reverse transcribed into
its DNA complement (complementary DNA, or cDNA) using
the enzyme ... of RT-PCR
44
o
C, 1hr
92
o
C, 10min
4
o
C, 0min
Step 1: Making cDNA
Step 2: Regular PCR
Basic techniques in DNA
technology
Gene cloing
Báo cáo Y học: Substrate specificity of human kallikrein 2 (hK2) as determined by phage display technology docx
... restriction enzymes
(Roche Biosciences; Amersham Pharmacia), PWO DNA
polymerase and shrimp alkaline phosphatase (Roche Bio-
sciences), T4 DNA ligase (Invitrogen), T4 polynucleotide
kinase (Promega), ... for substrate phage library.
The BamHIandXbaI/HindIII DNA recognition sites were
introduced by PCR onto 5¢ and 3¢ ends, respectively, of the
cDNA encoding the CFP fluorescent protein. The...
TUAN 2 tiet 3,4.docx
... thước đo góc , giấy kẻ ô vuông cho các bài tập11,14,19.
- Bảng phụ: Bảng 1: Ghi ?2 SGK ; Bảng ph 2: Ghi?3 SGK
2. Học sinh :
- Thước chia khoảng, thước đo góc
- Nắm vững đònh nghóa và tính chất ... 110
0
, góc N =
70
0
, góc S = 90
0
.
c) Hai góc đối đỉnh của
hình thang cân thì bù nhau.
12
/
H 2: Tính chất hình thang
cân:
HĐ2.1: Đònh lí 1
GV: Vẽ hình thang cân ABCD,
cho HS đo độ dài 2...
Tài liệu ADC KRONE - Guide - Fundamentals of Ethernet Technology docx
... WHITE PAPER
Fundamentals of
Ethernet Technology
Fundamentals of
Ethernet Technology
This white paper provides a brief tutorial on Ethernet – a family of ... Important Not Important
Fundamentals of Ethernet Technology
DCE
Packet
A
Packet
B
DCE
Figure 2. Packet-Switched Network
Packet-Switching: A Data-Friendly Technology
Differing requirements drove the ... only
data, but voice and...
Tài liệu Module 2: Web Service Architectures docx
... Architecture 9
The Web Services Programming Model 18
Review 21
Module 2: Web Service
Architectures
4 Module 2: Web Service Architectures
The preceding three service-oriented architecture ... robust
distributed applications,
there are a number of
requirements that must be
met.
Module 2: Web Service Architectures 1
Overview
!
Service-Oriented Architecture
!
Web S...
Tài liệu ADC’s Hardened Connector Technology docx
... CASE STUDY
Richland-Grant Telephone Cooperative Relies on
ADC’s Hardened Connector Technology to Achieve
Fast, Low-Cost Network Installation
BACKGROUND
Established in 1951, Richland-Grant ... lowest cost
possible. To meet its delivery goals, it’s important that RGTC keep its
communications technology on the cutting edge of emerging trends.
Creating an efficient, scalable infrastruct...
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