production of biopharmaceuticals from recombinant dna technology

... (2C5M4NP) was obtained by custom order from Chemical Soft R&D Inc (Kyoto, Japan) 20 mg of each of the chemicals is dissolved into L of distilled water and mg-Cl/mg-C of sodium hypochlorite was added ... quadrupole GC-MS, productions of two CBPs from fenitrothion were studied As shown in Fig 1(A), productions of fenitrooxon (peak a) and 3M4NP (peak b) in the sample of chlorinated fenitrothion were ... observed in the mass spectrum, production of trichloro-3-methylphenol (TC3MP) in the sample of chlorinated fenitrothion was inferred To confirm the production of TC3MP, 3M4NP was chlorinated...

Ngày tải lên: 05/09/2013, 10:15

... steps used to clone DNA?     Isolate DNA from an organism Cut the organismal DNA and the vector with restriction enzymes making recombinant DNA Introduce the recombinant DNA into a host Restriction ... 8.8 The synthesis of double-stranded complementary DNA (cDNA) from a polyadenylated mRNA, using reverse transcriptase, RNase H, DNA polymerase I, and DNA ligase Cloning of cDNA using BamHI linkers ... making of many copies of a segment of DNA Cloning vectors range from plasmids to artificial chromosomes to viruses Genomic libraries are collections of clones that contain at least one copy of every...

Ngày tải lên: 13/03/2014, 19:33

... Rectory, near Longfield End of the Project Gutenberg EBook of The Production of Vinegar from Honey, by Gerard W Bancks *** END OF THIS PROJECT GUTENBERG EBOOK THE PRODUCTION OF VINEGAR *** ***** This ... in respect of the material employed or in the process of manufacture of so important an article of consumption, surely deserves to receive the most careful attention.[Pg 8] MODE OF PRODUCTION ... for copies of or providing access to or distributing Project Gutenberg-tm electronic works provided that - You pay a royalty fee of 20% of the gross profits you derive from the use of Project...

Ngày tải lên: 22/03/2014, 14:20

... using recombinant DNA technology was human insulin, developed by Genentech and Licensed by Eli Lilly and Company  Applications of recombinant biotechnology  Recombinant human insulin  Recombinant ... describing the successful production and intracellular replication of recombinant DNA appeared in 1972 and 1973 Stanford University applied for a US patent on recombinant DNA in 1974, listing the ... Can carry up to 25kbDNA  1000 time more efficient than a plasmid Cloning in Lambda Vectors Make a partial digest of DNA Ligate the DNA to the arms of the vector Package the DNA into phage particles...

Ngày tải lên: 23/03/2014, 22:20

... wastewater of more than 85%, on one hand, and the production of a valuable energy source, on the other, yield two economic advantages Thus, an innovative technology for the production of energy from ... new reactor of 24 days The main fermenter has an absolute volume of 430 L, and therefore, a needed input of 18 L of desizing liquor per day resulted in a calculated biogas production of 486 L/day ... energy from wind, water, and sun power, the generation of energy from organic materials - especially undissolved solid residuals from agriculture - plays the most important role in the production of...

Ngày tải lên: 20/06/2014, 21:20

... concentration of glucose was higher for both biomass when the concentration of 4.5M H2SO4 was used The glucose yield of maize cobs was higher than that of groundnut shells, and ranged from to 0.89g/cm3 ... 4M, 4.5M and 5M of H2SO4 and the various acid concentrations recorded Using the best concentration of H2SO4 (4.5M), a mixture of maize cobs and groundnut shells in the ratio of 1:1, 1:2, 2:1, ... could produce high quality of ethanol To determine the effect of different acid concentrations on the hydrolysis 2M solution of concentrated H2SO4 was prepared and 10g of the leached maize cobs...

Ngày tải lên: 06/07/2014, 18:20

... quality of RCA ix Microwave-Assisted Production of Aggregates from Demolition Debris LIST OF FIGURES  Figure 1.1 Price of Granite in Singapore Market (Statlink, 2010) 15 Figure 2.1Share of mortar ... Mechanical Properties of Concrete 267  vii Microwave-Assisted Production of Aggregates from Demolition Debris ABSTRACT  Concrete recycling is an increasingly common method of disposing of demolition ... the best of all things ii Microwave‐Assisted Production of Aggregates from Demolition Debris  TABLE OF CONTENTS    ACKNOWLEDGEMENT ii  ABSTRACT viii  LIST OF FIGURES...

Ngày tải lên: 11/09/2015, 10:02

... University of Technology, Goteborg, Sweden 23 Walia N.K., S.S Bedi, K Kundu, R Karmakar 2013 Production of Bioethanol From Mango Peel International Journal of Engineering Research & Technology ... Objectives: Production of ethanol from mango peel using Saccharomyces cervisiae Activities in the research: - Study on the effect of the concentration of H2SO4 on hydrolysis - Study on the effect of time ... The determination of acid’s suitable concentration was very important to the efficiency of ethanol production from mango peel The low level of acid (0.5%) led to low performance of hydrolysis whereas...

Ngày tải lên: 06/10/2015, 13:05

... of E coli in plate assay using 100 pmol of each peptide, complete disappearance of ferroportin signal at the membrane of iron treated macrophage J774 cells in the presence of 700 lM of each of ... microlitres of hepcidin (0.1 mgÆmL)1) were eluted with the following gradient at mLÆmin)1 from 90% of (H2O, 0.1% TFA) and 10% of (H2O 30%, CH3CN 70%, TFA 0.1%) to 10% of (H2O, 0.1% TFA) and 90% of (H2O ... 6.2 (instead of pH for m1hepc) and the UnoQ chromatography step was run at pH 6.2 rather than pH The production yield of m1hepc was slightly lower than that of huhepc (8% instead of 13%, respectively,...

Ngày tải lên: 18/02/2014, 18:20

... a processive mode of action One goal of the present study was to exploit the unique heteropolymeric nature of chitosan to study the mechanism of action of ChiB A second goal of this study was ... al Degradation of chitosans with ChiB Fig Time course of ChiB degradation of a chitosan with FA ẳ 0.65 The graph shows the degree of scission (a) as a function of time The slope of the line at ... value of a is 0.5 and that the presence of D-units lowers the maximum value of a) FEBS Journal 272 (2005) 538549 ê 2005 FEBS The size distributions of oligomers resulting from hydrolysis of FA...

Ngày tải lên: 07/03/2014, 16:20

... cells 36 DNA Methylation – From Genomics to Technology 2.4.4 Sequencing errors Another source of incorrect profiling of the methylation state is the erroneously calling of a thymine instead of a ... inherited cause of intellectual disability The third part of the book is dedicated to analysis of role of DNA methylation in cancer According to the American Cancer Association, nearly 13% of all deaths ... favoured loci of “foreign” DNA insertions, which tend to disturb the existing DNA methylation patterns, (Collins et al., 1998) Information from literature indicates that a majority of DNA methylation...

Ngày tải lên: 08/03/2014, 19:20

... other types of water production problems 5.1.1.2 Water Shut-Off Chemicals Another approach to shutting off water production while allowing continued production of oil involves the use of chemicals ... oil and gas production, the produced water from a CBM well comes in large volumes in the early stages of production; as the amount of water in the coal decreases, the amount of methane production ... federal waters of the Gulf of Mexico seaward of the outer boundary of the territorial seas offshore off Louisiana and Texas The general permit includes: - No discharge within Area of Biological...

Ngày tải lên: 09/03/2014, 01:20

... find the gene of interest DNA pieces are joined in vitro to form recombinant molecules • Generate sticky ends on the DNA, e.g with restriction endonucleases • Tie DNA molecules from different ... anneal primers, and synthesize new DNA: 14 duplex molecules of desired product PCR: make large amounts of a particular sequence • The number of molecules of the DNA fragment between the primers ... to specific DNA segments • Polymerase Chain Reaction • Requires knowledge of the DNA sequence in the region of interest • As more sequence information becomes available, the uses of PCR expand...

Ngày tải lên: 13/03/2014, 18:39

... substrate of alkaline phosphatase Materials and methods Bacterial identification and culture conditions DNA manipulation and sequence analysis Plasmid DNA preparation, purification of DNA from agarose ... 3¢-terminal sequence of Br thermoruber 16S rDNA In the light of this identity with the RBS, we found an initiation codon (TTG) from bp downstream of RBS, which is followed by a long ORF of 2337 bp Consequently, ... C, Control experiment, DNA only those of E coli Lon (37 °C) In addition, the effect of temperature on the DNA- binding activity of Bt-Lon was examined by EMSA after 20 of incubation at 25, 35,...

Ngày tải lên: 16/03/2014, 16:20

... Percentage of H-chain to sercin-1 0.0 4.4 ± 0.7 2.9 ± 0.1 a Copy numbers of mRNAs of L-chain and H-chains of the recombinant IgG, and sericin-1 per 10 ng of total RNA The indicated values are 10)5 of ... obtain 1.2 mg of purified mAb from 500 mg of L ⁄ H ⁄ IE1-line cocoons Antigen-binding properties of recombinant mAb The IgG L- and H-chain cDNAs used in the present study were cloned from a mouse ... L-chain monomer of the IgG, respectively Table Purification of the recombinant mAb from 500 mg of cocoons Purification step Amount of mAb (mg) Recovery (%) Cocoons Extract Eluate from protein G...

Ngày tải lên: 23/03/2014, 04:20

... therefore prepared from proteins blotted from SDS gels to Ó FEBS 2004 Native and recombinant chlorite dismutase (Eur J Biochem 271) 3541 Fig Separation of tryptic peptides of native (A) and recombinant ... of tryptic digests from the native and recombinant enzymes Migration times in this type of analysis are prone to variability [29], but most of the peptide peaks seen in the electropherogram of ... in the recombinant version of the enzyme Comparison of mass spectra for tryptic peptides obtained from the native and recombinant enzymes suggest HK(52– 53) and YHVR(220–223) as sites of modification...

Ngày tải lên: 23/03/2014, 12:20

... terms of the proper assignment of words to part -of- speech classes However, it must be capable of separating identically spelled words into different classes on the basis of function These words often ... distinctions, the current version of the parser fails to supply this information A new version of UTTER should include a better parser which is capable of making these sorts of part -of- speech dlstlnctlons ... SYSTEM ORGANIZATION The current implementation of UTTER operates in one of three modes, each of which corresponds to one of the three tasks required of the system: (I) (2) trainin~ mode: flagglr~...

Ngày tải lên: 24/03/2014, 05:21

... one of the most popular vehicles It is due to easy scalability and high levels of expression According to Farrell and Iatrou (2004), a hundred milligrams of recombinant protein per liter of culture ... transfectional ability of virus, the transgene is delivered into animal cells as part of a recombinant viral genome (Primrose and Twyman, 2006b)  Bactofection This method involves in the use of bacteria ... system has been used widely for production of recombinant proteins due to following reasons:  They could process authentic post-translational modifications of recombinant proteins, which bacteria...

Ngày tải lên: 28/03/2014, 09:20

... overexpression of human PRDX5 in mitochondria of Chinese hamster cells decreases formation of 8-oxoG in mitochondrial DNA [20], but the role of this protein in protecting nuclear DNA from oxidation ... with ASAT primers of identical amounts of cDNA (1 : 25 dilution) from the control clone (curve P) and from the KD-1 clone (curve D) (C) Fold difference between cDNA isolated from the control and ... inhibition of PRDX5 by CSE can be explained by a CSE-induced decrease in NRF2 (Fig 3C,D) Role of PRDX5 in suppression of DNA oxidation (formation of 8-oxoG) To study the potential significance of PRDX5...

Ngày tải lên: 30/03/2014, 11:20