Báo cáo khoa học: Functional site of endogenous phospholipase A2 inhibitor from python serum Phospholipase A2 binding and anti-in¯ammatory activity ppt

... Functional site of endogenous phospholipase A 2 inhibitor from python serum Phospholipase A 2 binding and anti-in¯ammatory activity Maung-Maung Thwin 1 , Ramapatna ... IC 50 , concentration of the inhibitor that inhibits PLA 2 activity by 50%; PIP, phospholipase inhibitor from python; PLI, phospholipase A 2 inhibitor; sPLA 2 , secretory phospholipa...

Ngày tải lên: 23/03/2014, 21:21

... ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been described from all three domains of life [1,2], and usually ... constitute a large and widespread superfamily of b-barrel proteins in which a mononuclear metal site is both a conserved feature of the struc- ture and a source...

Ngày tải lên: 18/02/2014, 06:20

... between helices 3 and 4 at the junction crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would ... well-defined dimer. The cata- lytic site consisted of two aspartates ) Asp46 and Asp230 ) which mapped to the catalytic site of RuvC. Protruding from opposite sides...

Ngày tải lên: 20/02/2014, 11:20

... vitro,an activity characteristic of molecular chaperones and shown here to be shared by apoferritin and ferritin. This is the first report that apoferritin ⁄ ferritin may protect cells from stress ... denaturation of citrate synthase in vitro,a capability shared by apoferritin and ferritin, and con- fers stress tolerance on transfected mammalian cells. Artemin and ferritin ma...

Ngày tải lên: 16/03/2014, 11:20

... be built, suggesting conservation of ligand and metal binding sites. Comparison with corresponding sequences from a variety of organisms shows more than 1 ⁄ 6 of the sequence is completely conserved. Mutants ... sequence from P. Sher- manii (MCEE_PROFR) are also labeled. Fig. 6. Model of MCE-1 structure. View along the length of the eight-stranded beta-barrel onto the putati...

Ngày tải lên: 23/03/2014, 13:20

... Baker EN (2003) Structural and functional consequences of binding site mutations in transferrin: crystal structures of the Asp63Glu and Arg124Ala mutants of the N-lobe of human transferrin. Biochemistry ... absence or presence of increasing concentrations of unlabeled Ggly. The ApoTf–Ggly complex was separated from the unreacted Ggly by SDS ⁄ PAGE, and the extent of...

Ngày tải lên: 07/03/2014, 02:20

... between the base and substrate -binding amino acid affected the binding of dThd and deoxycytidine (dCyd). Results We performed site- directed mutagenesis of four active site residues of Dm-dNK. Residue ... Structural alignment of wild-type Dm-dNK (blue) (PDB ID: 1OE0) and Dm -dNK-E52D (red) illustrating the binding of the feed- back inhibitor dTTP and the position...

Ngày tải lên: 19/02/2014, 02:20

... levels of amino acid sequence similarity ranging from 45% to 53%, the architecture of the aglycone -binding site of Zm-p60.1 differs distinctly from that of Bgl4:1 and its homologs. These findings offer ... analysis of structural and catalytic prop- erties of the mutants, and simulations of the sub- strate–enzyme interactions of the wild-type and one of the mut...

Ngày tải lên: 16/03/2014, 04:20

... quantitation of microgram quantities of protein, utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. 38 Segel IH (1993) Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and ... may act as an allosteric inhibitor of m-NAD-ME [18,21,25], and the allosteric properties of this isoform may relate to its particular role in the pathways of mala...

Ngày tải lên: 23/03/2014, 06:20

... other h and, deletion of up to 273 residues from the C-terminal region of DEC1 (DEC1:1–309, DEC1:1–232 and DEC1:1–139) had little effect on the suppressive activity of DEC1. Expression of these ... resulted in adequate levels of expression. The transcriptional activity of FLAG-BMAL1, and the suppressive activity of FLAG- DEC1, were s imilar to those of intact BMA...

Ngày tải lên: 19/02/2014, 16:20