Báo cáo khoa học: The specific delivery of proteins to human liver cells by engineered bio-nanocapsules doc
Báo cáo khoa học: The specific delivery of proteins to human liver cells by engineered bio-nanocapsules doc
... fluorescent protein (EGFP) at the C-terminus of the S region was designed to deliver proteins to human hepatocytes. Truncation of the C-terminus of the S region was required to obtain suffi- cient expression ... applied to various proteins for the C-terminal moiety of the L fusion proteins, depending on the character of the proteins, such as cytoplasmic...
Ngày tải lên: 23/03/2014, 15:20
Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... be increased by 40-fold to mimic the specific activity of chitosanase. Thus, the results add value to otherwise commercially available raw materials, viz. chitosan from the offal of marine food ... An examination of DA values indicates that the total DA of the products is equal to that of native chitosan (19% for LMWC and 31.2% for chito-oligomers + monomer, th...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
... suppression of PMLA synthesis. Among other possibilities, this effect on PMLA synthesis could be the result of loss of induction at the transcriptional level, of loss of activation of the synthetase ... and references therein), suggesting the presence of detect- able amounts of PMLA synthetase -specific mRNA. The absence of PMLA and polymalatase in other cell types...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... phosphorylation of MCM4, together with the presence of geminin, which inhibits the ability of CDT1 to load MCM proteins onto chromatin, may help prevent re-replication of DNA in the G 2 and M phase. ... 33 °C) of the intensity of the signals is shown. (B) A dominant-negative mutant of human CDK2 was expressed as fusion proteins with HA in HeLa cells. The eff...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction doc
... either DNA strand. Moreover, there was no difference between the Rat-1 samples and the untreated FTO-2B hepatoma cells. After the addition of hormones to FTO-2B cells, however, the reactivity of ... sta- bilization of GR binding to the GRU, and thereby stabilization of the interaction of the coregulators interacting with the nucleoprotein complex formed at the...
Ngày tải lên: 23/03/2014, 10:20
Báo cáo khoa học: The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication ppt
... substrate the G:T mispair can be extended either by direct incorporation of a G opposite the next C in the template or by misalignment of the primer-terminal G with the next C followed by the incorporation ... incorporation, cal- culated by dividing the amount of product formed by the reaction time. The observed rate was plotted as a function of the dNTP...
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
... cross-sections of L3 larvae with antibody to GFP revealed signals in the cytoplasm of muscle cells, the source of MIG-17 expression, and on the surface of the gonad and the intestine (Fig. 4A). The poly- clonal ... with Monoclonal 2 and the antibody to the prodomain revealed that the signal colocalized to the basement membrane of the muscle and the gon...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements ppt
... features of the proteins encoded by these genes. To determine the differences between these enzymes, we compared the expression patterns of each RNase H1-related gene throughout the development of the ... supports the idea that the dsRHbd of Ce-RNH1a does not affect the specificity of the cleavage site. To compare the enzymatic activities of Ce-RNH1a and...
Ngày tải lên: 30/03/2014, 11:20
Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... pro- trudes towards the exterior of the tetramer near loop 275–288 of the opposite monomer. Therefore, the binding and adaptation of this portion of SsCU-NH to the active site of the protease ... [68], by rolling a probe atom of 1.40 A ˚ on the van der Waals sur- face of the protein model. The analysis of cavities in the proteins was made using the...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt
... maturation of Toc75 in the pea Fig. 1. The biogenesis of pea Toc75. The precursor, intermediate, and mature forms of Toc75 are indicated as prToc75, iToc75, and mToc75 with the numbers of the N-terminal ... 4B,C). Import of GGE mutant was less efficient than that of other proteins, as evidenced by the accumu- lation of the precursor form of Toc75 that was sensi...
Ngày tải lên: 19/02/2014, 07:20