Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identiﬁcation of sulfated high-mannose type oligosaccharides doc

... (2005) 3803–3815 ª 2005 FEBS 3815 Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identiﬁcation of sulfated high-mannose type oligosaccharides Mariana Barboza1, ... Cazzulo JJ(1993) The reactivity of sera from chagasic patientsagainst different fragments of cruzipain, the major cystein proteinase of Trypanosoma cruzi , suggests the presence of deﬁned antigenic ... Fig. 6B).Altogether, these data conﬁrm the presence of sul-fated high-mannose type oligosaccharides in cruzipainand in its C-terminal domain. This is the ﬁrst report of the use of nor-harmane...

Báo cáo khoa học: Structural analysis ofN-glycans of the planarian Dugesia japonica. ppt

... was shown that the N-glycans of planarian include high mannose -type and pauci-mannose- type glycans. However, many of the major N-glycans from planarians havenovel structures, as their elution ... map. The diam-eter of the circles is proportional to the cube root of the detectedamount of PA-glycans. The positions of standard PA-glycans areindicated by crosses labeled (a)–(g). The standards ... hypothesis, it isnecessary to compare the genes involved in the synthe-sis of N-glycans in many organisms. The hypothesiswould gain strong support if lower metazoans werefound to have the...

Báo cáo khoa học: Structural analysis of the jacalin-related lectin MornigaM from the black mulberry (Morus nigra) in complex with mannose ppt

... conformation of loop 99–107 (L2) in the Aprotomer as compared to its conformation in the B, Cand D protomers of the tetramer. This loop forms the roof of the sugar binding pocket of MornigaMand ... gJRL. The size and shape of the carbohydrate-binding site of MornigaM and other JRL essentially depends onboth the conformation of the three loops that delineate the binding cavity and the presence ... by the side chain of Lys106 which protrudes from loop 2to close up the cavity. These structural discrepancies of the carbohydrate-binding cavity should have a pro-found inﬂuence on the type of...

Báo cáo khoa học: Structural analysis of lipopolysaccharides from Haemophilus inﬂuenzae serotype f Structural diversity observed in three strains ppt

... the steps in the biosynthesis of the oligosaccharide portion of the LPSmolecules. The genes lpsA, lic2A and lgtC encodeglycosyltransferase enzymes involved in the addition of the globoside ... to Hex8)were of greater abundance in the spectrum of RM6252 thanin the one of RM7290. From ESI-MS data it was alsoevident that PCho was not a major substituent in the LPS of the type f strains.Characterization ... (B) The MS3spectrum of the fragment ion m/z 1613.8resulting from the MS2 of m/z 2283.6. The relevant fragmentation pathways are shown to the right of each spectrum.Fig. 3. The ESI-MSnanalysis...

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... bond, linkage of the activesite Ser of the protease to the carboxyl group of P1by anester bond and insertion of the N-terminal part of the RCLas strand 4 in b-sheet A (s4A) of the serpin. ... cognateproteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing structural rearrangements of the serpin stabilizes the molecule in a so-called ... critical position at the top of the hF/s3A-loop right above the inserted s4A while in the stressedconformation of PAI-1staband a1PI, they stack against the aromatic moiety of the s3A residues...

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... comprising the N-terminal fragment and the ﬁrst AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas-settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... activation of the reporter gene and thus is sufﬁ-cient to maintain the interaction with Pex1p. Theseﬁndings demonstrate the importance of the ﬁrst AAA-cassette together with the N-terminal region of ... attention to the cassette structure of the proteins. We assigned the binding sites of thesetwo AAA-proteins to their different protein regionsand elucidated the importance of ATP-binding to the two...

Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate speciﬁcity doc

... assumes that the thiol group of Cys135 adds to C6 of the pyrimidine ring, favoring the exit of the aminic group. The subsequent addition of a water molecule and the expulsion of the active cysteine ... superposition of the Caatoms of one monomer with that of the other members of the family gives a rmsd of 1.7, 1.3 and 1.4 A˚formodels 1RTW, 1UDD and 1TYH, respectively. The major differences ... derivative. The presence of a limited number of enzymesinvolved in the thiamin biosynthesis in H. pylori, and the peculiar environment in which it thrives in, leadsto the hypothesis of the existence of...

Báo cáo khoa học: Structural adaptation to low temperatures ) analysis of the subunit interface of oligomeric psychrophilic enzymes pdf

... The trend was strengthened by the inclusion of thermophilic enzymes in the comparison.Figure 2 shows the percentage of apolar interface,Table 3. Statistical analysis of the differences in the ... using a set of 512 points locatedon a sphere around each atom. The sphere interaction radius of each atom is equal to the sum of the van der Waals radius of the atom type plus the radius of a water ... for the structural property if the interfaces of two homologous mesophilic enzymes werecompared. The t-test should then establish whether the magnitude of the differences detected between the...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... respectively, of that of HPRT. This low activitycould be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... andregulators of other enzymes in the human nucleotidemetabolism (Table S1). The library can then be seen toconstitute the speciﬁc metabolome of the nucleotidemetabolism, which would be the most ... interacts with bothO6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved in coordinating...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... inNOS, the second electron is provided to the heme-dioxy species by a bound H4B cofactor rather than by the ﬂavoprotein domain [16]. The H4B radical is thenreduced within the enzyme by the ... cytochrome c reduction measurements.c The effect of the element is inferred from deletion mutants,therefore the effects reported in the table are the opposite of the observed effects.dAll but one ... importantto further test the validity, kinetics and thermodynam-ics of the through-heme pathway in NOS enzymes.ConclusionsAlthough the NOS ﬂavoprotein domain has fundamen-tal structural, thermodynamic...

Xem thêm