Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc

... (2005) 3803–3815 ª 2005 FEBS 3815 Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides Mariana Barboza 1 , ... Cazzulo JJ (1993) The reactivity of sera from chagasic patients against different fragments of cruzipain, the major cystein proteinase...

Ngày tải lên: 23/03/2014, 15:20

... was shown that the N-glycans of planarian include high mannose -type and pauci-mannose- type glycans. However, many of the major N-glycans from planarians have novel structures, as their elution ... map. The diam- eter of the circles is proportional to the cube root of the detected amount of PA-glycans. The positions of standard PA-glycans are indicated by c...

Ngày tải lên: 28/03/2014, 23:20

... conformation of loop 99–107 (L2) in the A protomer as compared to its conformation in the B, C and D protomers of the tetramer. This loop forms the roof of the sugar binding pocket of MornigaM and ... gJRL. The size and shape of the carbohydrate-binding site of MornigaM and other JRL essentially depends on both the conformation of the three loops that delineate...

Ngày tải lên: 30/03/2014, 20:20

... the steps in the biosynthesis of the oligosaccharide portion of the LPS molecules. The genes lpsA, lic2A and lgtC encode glycosyltransferase enzymes involved in the addition of the globoside ... to Hex8) were of greater abundance in the spectrum of RM6252 than in the one of RM7290. From ESI-MS data it was also evident that PCho was not a major substituent in t...

Ngày tải lên: 23/03/2014, 21:20

... bond, linkage of the active site Ser of the protease to the carboxyl group of P 1 by an ester bond and insertion of the N-terminal part of the RCL as strand 4 in b-sheet A (s4A) of the serpin. ... cognate proteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing structural rearrangements...

Ngày tải lên: 20/02/2014, 11:20

... comprising the N-terminal fragment and the first AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas- settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... activation of the reporter gene and thus is suffi- cient to maintain the interaction with Pex1p. These findings demonstrate the importance of the first AAA- cassette...

Ngày tải lên: 07/03/2014, 16:20

... assumes that the thiol group of Cys135 adds to C6 of the pyrimidine ring, favoring the exit of the aminic group. The subsequent addition of a water molecule and the expulsion of the active cysteine ... superposition of the Ca atoms of one monomer with that of the other members of the family gives a rmsd of 1.7, 1.3 and 1.4 A ˚ for models 1RTW, 1UDD an...

Ngày tải lên: 16/03/2014, 00:20

... The trend was strengthened by the inclusion of thermophilic enzymes in the comparison. Figure 2 shows the percentage of apolar interface, Table 3. Statistical analysis of the differences in the ... using a set of 512 points located on a sphere around each atom. The sphere interaction radius of each atom is equal to the sum of the van der Waals radius of the...

Ngày tải lên: 30/03/2014, 03:20

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... and regulators of other enzymes in the human nucleotide metabolism (Table S1). The library can then be seen to constitute the specific metabolome of the nuc...

Ngày tải lên: 15/02/2014, 01:20

... in NOS, the second electron is provided to the heme- dioxy species by a bound H 4 B cofactor rather than by the flavoprotein domain [16]. The H 4 B radical is then reduced within the enzyme by the ... cytochrome c reduction measurements. c The effect of the element is inferred from deletion mutants, therefore the effects reported in the table are the opposite of the...

Ngày tải lên: 18/02/2014, 11:20