Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

... 399-fold from the tissues of carnation (Dianthus caryophyllus L). The enzyme, with a molecular mass of 43–45 kDa and a pI of 4.15, specifically methylates the hydroxy substituent in 4¢-position of the ... mL of the above mentioned culture medium: a total of 400 dishes were prepared and used in the further steps of the experiments. Fungal material Fod pathotype...

Ngày tải lên: 08/03/2014, 08:20

... FEBS Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans William G. Willmore and Kenneth B. Storey Institute of Biochemistry, Carleton ... MJ (2002) Comparative study of the purification and char- acterization of the cytosolic glutathione S-transferases from two salmonid specie...

Ngày tải lên: 23/03/2014, 15:20

... composed of two each of the a and b subunits. Analysis of the N-terminal transit peptide of CLGAT Analysis of the CLGAT cDNA predicted a chloroplast transit peptide upstream from the N-terminus of CLGAT. ... clone of the GAT, gene- specific primers were designed from the sequence of watermelon EST AI563351 and used for 5¢- and 3¢-RACE. The cloned cDNA en...

Ngày tải lên: 20/02/2014, 03:20

... constit- uents of the cell surface. They are found at the outermost ends of the sugar chain of animal glycoconjugate. They act as an important component of the ligands recognized by the lectins. Recognition ... microtiter well and mixed. The values of the HAI titer were determined after 60 min of incubation and expressed as the highest dilution of sugars that...

Ngày tải lên: 21/02/2014, 00:20

... cloned into the vector pQE16 in frame with the His-tag-encoding sequence. The lepB gene was PCR ampli®ed from E. coli DNA using the primers Lep1 and Lep2 (Table 2), and then cloned into the pQE16 ... A BLAST search of the nucleotide sequence of the ampli®ed DNA fragment revealed the presence of three ORFs, the deduced proteins having 70, 77, and 67% identity to...

Ngày tải lên: 21/02/2014, 03:20

... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. ful- gidus enzyme is still unknown. Therefore, no substrate could be added to the titration ... enzyme complex from A. fulgidus (Eur. J. Biochem. 269) 1897 Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-t...

Ngày tải lên: 21/02/2014, 03:20

... sequences of ZP2 and ZP3 deduced from their cDNAs. The arrows and capital letters (sites A to F) indicate the cleaving sites of ZHE1 determined from the N-terminal amino acid sequences of the ZHE1 ... upstream from the cleaving site obtained from the 43 kDa product of ZP3 (site E). Therefore, the finding that the 36.5 kDa prod- uct is a mixture of two pept...

Ngày tải lên: 07/03/2014, 04:20

... Fractionation and purification of the enzymes stored in the latex of Carica papaya. J Chrom B 790, 229–238. 32 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage ... content in their latex; and (2) the pres- ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protei...

Ngày tải lên: 07/03/2014, 11:20

... CBCP from T. congolense is an atypical CBCP in terms of its enzymatic properties. Therefore, TcoCB6 and daikon CBCP are distinct from one another in terms of the role of the one histidine in the ... electrophoresis and N-terminal sequence of the purified enzyme. (A) Native PAGE of the purified enzyme (0.5 lg) in the absence of SDS. (B) SDS ⁄ PAGE of the puri...

Ngày tải lên: 16/03/2014, 04:20

... After 1 h the reaction was stopped by the addition of 750 lL of acetic acid, and the color developed by the addition of 250 lL of ninhydrin (4 mgÆmL )1 )at 95 °C for 1 h. A standard curve of different ... Alabama Department of Microbiology and Immunology and the College of Medicine, by Public Health Service grant CA101931 (to D.J.M.) from the National Institu...

Ngày tải lên: 16/03/2014, 16:20