Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot

... method, the inhibitor competes with a high excess of substrate for the active sites of the enzyme; therefore, the slow-onset phase of the reaction may not always be observed [18]. This is shown in the ... time, the fluorescence of the solution may be represented as the sum of the fluorescence of the various molecular species present in the mixture, free t...

Ngày tải lên: 29/03/2014, 08:20

... implications of the observed VpUcyt structure One of the most studied activities of VpU is the induc- tion of CD4 degradation in the endoplasmatic reticu- lum of infected CD4 + cells. Only the cytoplasmic domain ... highly attractive research topic and may be studied by solid state NMR methods. However, the objective of the present study was to gather structural...

Ngày tải lên: 18/02/2014, 06:20

... strikingly different conformations in the structures of the isolated b¢ domain and full-length proteins. In the crystal structure of the b¢x fragment of human PDI, the C-terminal part of the x-linker turns back ... Binding of PDI to the prolyl-4-hydroxylase complex minimally requires intact b¢ and a¢ domains of PDI, but the assembly and activ- ity of the...

Ngày tải lên: 15/03/2014, 23:20

... McGough and coworkers [20] and subsequently by others [31], and the thrusting forward of subdomain 2 with respect to the rest of the monomer. In this report we study the pH-dependence of the actin– cofilin ... controlled by the interplay of a variety of signaling pathways. The importance of the contribution of ADF/cofilins to cell motility is reflected in thei...

Ngày tải lên: 31/03/2014, 09:20

... the first hypothesis and therefore a direct involvement of PHI in electron transfer. This conclusion is consistent with the very low degree of identity between the N-terminal sequence of PHI and ... one PHR and one PHO (abc) protomer. The hypothesis of a direct PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter...

Ngày tải lên: 21/02/2014, 00:20

... between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b and cENaC, leading to channel ubiquitination, internalization and degradation ... donor (Fig. 8A), the inhibitory effects of heparin and DPG (Fig. 8B), the fact that purified CK2 can phosphorylate bS631 and cT599 (Fig. 7A), the presenc...

Ngày tải lên: 21/02/2014, 03:20

... prosecuted the queries on a remote computer, and responded with the requested information in a readable format tailored to the characteristics of the answer. The basis for the development of the ... determine whether they interact with the different parsers and/ or each other, whether any gains are offset by introduced overhead, and whether the source and p...

Ngày tải lên: 08/03/2014, 18:20

... to +1157 relative to the AUG of the psbA mRNA (corresponding to the coding region of the C-terminal amino acids of D1 and the 3¢ UTR of the psbA mRNA) was amplified with the oligonucleotides psbA3/1 ... used in the binding assays shown in Fig. 3C) and the predominant, shorter form with a leader of 36 nucleotides (Fig. 5A) [34,35]. It has been hypothesized that...

Ngày tải lên: 17/03/2014, 11:20

... whether the enzyme requires one or two metal ions for activity, because the number of the metal-binding sites found in the crystal structures of the enzyme–metal ion complex formed in the absence of ... absence of the substrate varies for different enzymes and different metal ions. For example, single Mg 2+ [43] and Mn 2+ [44] ions bind to the active site...

Ngày tải lên: 30/03/2014, 02:20

... is a soft ligand and therefore shows a preference for soft metals. More- over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ion. ... 2 forms could yield information on the structural and chemical features responsible for the biological peculiarities of MT3, such as its growth-inhibitory activity....

Ngày tải lên: 16/02/2014, 15:20