Báo cáo khoa học: Biosynthesis of isoprenoids A bifunctional IspDF enzyme fromCampylobacter jejuni pot

... falciparum. Comparative genomic analysis show that all sequenced a- ande-proteobacteria have fused ispDF genes. These bifunctional proteins a re potential drug targets in several human pathogens ... tuberculosis and Mycobacterium leprae as well as in the v arious Plasmodium species causing malaria. Recent studies have already shown that malaria can be treated by the antibiotic fosmidom...

Ngày tải lên: 23/03/2014, 13:20

... novel antimalarial agents, which are urgently needed in light of the enormous death toll of malaria [23] and the rapid dissemination of variants with resis- tance against currently available drugs ... Plasmodium falciparum malaria. Antimicrob Agents Chemother 50, 2713–2718. 23 Hoffman SL, Subramanian GM, Collins FH & Venter JC (2002) Plasmodium, human and Anopheles genomics and mala...

Ngày tải lên: 16/03/2014, 06:20

... times revealed that this band was composed of three or four different m olecular mass f orms. Parallel a nalysis of the supernatants revealed a positive signal starting at 60 min of chase a nd having a ... metho trexate. Analysis of permeabilized cells by confocal microscopy revealed an intracellular pool of GPV with a granular appearance (Fig. 1B). Analysis of culture s...

Ngày tải lên: 23/03/2014, 13:20

... [26]. The branched arabinan chains of the arabinogalactan are attached to the linear galactan backbone. The arabinan consists of an inner linear region of Araf-(1 fi 5) -a- Araf and of branched non-reducing ... terminal Ara6 motifs: Arafb1 fi 2Arafa1 fi 5 (A rafb1 fi 2Arafa1 fi 3)Arafa1 fi 5Arafa1. About two-thirds of the terminal b-Araf and the penultimate 2 -a- Araf serve as attachment...

Ngày tải lên: 30/03/2014, 04:20

... was identified as an interaction partner for ADAM10 that enhances a- sec- retase shedding of APP, probably by regulating matu- ration of the prodomain of ADAM10 [22]. The catalytical domain of ADAM10 ... members of the ADAM family have been shown to act as a- secretase [8,36,37]: ADAM9, ADAM10 and ADAM17 (TACE). Overexpression of ADAM9 has been reported to increase the basal and...

Ngày tải lên: 16/02/2014, 09:20

... BamH1 sites of pQE60 (Table 1). The gene itself was slightly modi- fied by the addition of the DNA sequence motif 5¢-GAA TTCattaa agaggagaaattaact ATG AGA GGA TCT CAC CAT CAC CAT CAC CAT GGG ATC GAT CAT-3¢ ... cyclohydrolase II of B. subtilis that was performed using an enzyme ⁄ substrate ratio of 1 : 0.7. The reaction was characterized by a decrease in absor- bance at 252 nm and a...

Ngày tải lên: 18/02/2014, 11:20

... PstI and SalI CGGTTCACTAAACGAGCTCTGCTGCAGaaaaaatccaaaaaaaatctaaaaaaatcttttaaaa aaccccaaaaaaatttacaaaaaaGTCGACaatgc Antisense ARS with PstI and SalI gcattGTCGACttttttgtaaatttttttggggttttttaaaagatttttttagattttttttg gattttttCTGCAGCAGAGCTCGTTTAGTGAACCG TOP ... gcattGTCGACttttttgtaaatttttttggggttttttaaaagatttttttagattttttttg gattttttCTGCAGCAGAGCTCGTTTAGTGAACCG TOP Ccttctccccggcggttagtgctgagagtgc A...

Ngày tải lên: 18/02/2014, 12:20

... 5¢-TAATTAACCCTCACTAAAGGGGTGCTCGGCATAAG Reverse ompA105 5¢-GCCATGAATATCTCCAACGAG Reverse ompA117 5¢-CATCCAAAATACGCCATGAATATC Forward 5¢rpsO 5¢-TAATACGACTCACTATAGGGGCCGCTTAACGTCGCG Reverse 5¢rpsO 5¢-GCTTCAGTACTTAGAGAC Forward ... 5¢-GCTTCAGTACTTAGAGAC Forward 3¢rpsO 5¢-TAATACGACTCACTATAGGGAGACGTAGCACGTTACACC Reverse 3¢rpsO 5¢-GAAAAAAGGGGCCACTCAGG Reverse 3¢rpsO-(T)18 5¢-T(18)GAAAAAAGGGGCCACTCAG...

Ngày tải lên: 19/02/2014, 16:20

... 67, 5833–5839. 26 Wiater A, Szczodrak J & Rogalski J (2001) Purification and characterization of an extracellular mutanase from Trichoderma harzianum. Mycol Res 105, 1357–1363. 27 Hasegawa S & Nordin ... a- 1,3-glucanase; mycoparasitism; Trichoderma asperellum Correspondence E. Monte, Centro Hispano Luso de Investigaciones Agrarias, Universidad de Salamanca, Edificio Departamental,...

Ngày tải lên: 19/02/2014, 16:20

... that A B C shifted 123 123456 band Probe 1.00E+008 AAAGAGATG AAGGACATG AAAGACCTG AAAGACATG K d =1.2 nM AAAGAGATG K d =0.29 nM AAAGACACG K d =4 nM AAAGACACG AAAGACATA AGAGACATG AAAGACATG AAAGCCATG AAATACATG 8.00E+007 6.00E+007 4.00E+007 2.00E+007 0.00E+000 14 16 12 10 8 Fluoriscence 340 Fluoriscence 340 6 4 0.00 ... Average K d (nM) AAAGACATG + + 0.25 A GAGACATG ND – AA GGACATG ND – AAA TA...

Ngày tải lên: 07/03/2014, 10:20