Báo cáo khoa học: Functional transitions of F0F1-ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles pdf

... investi- gated in submitochondrial particles by studying the IF1- mediated ATPase inhibition in the presence and absence of a protonmotive force. In the presence of protonmotive force, IF1 added during ... Functional transitions of F 0 F 1 -ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles Mikhail G...

Ngày tải lên: 23/03/2014, 12:20

... immunoblotting data, it was of interest to re-examine the fate of cals equestrin by studying the entire complement of key Ca 2+ -binding elements in dystrophin- deficient skeletal muscle fibres. Because the ... n the expression of Ca 2+ -binding proteins. The relatively unique combination o f the pI value and molecular mass of individual 2D spots can be useful in th...

Ngày tải lên: 19/02/2014, 16:20

... (A) The bind- ing of [ 14 C]plactin-50 to human prothrombin was determined in the presence of the indicated concentrations of [ 14 C]plactin-50. Specific binding data are shown. (B) The intrinsic ... 4). These properties of the plactin–prothrombin interaction suggested a change in the conformation of prothrombin after plactin bind- ing. We measured the intrinsic fluo...

Ngày tải lên: 07/03/2014, 00:20

... by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by superoxide in the course of the reaction. The principle of ... catalysis, ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been de...

Ngày tải lên: 18/02/2014, 06:20

... in the size of the side chain may create steric hindrance for the substrate, making the base moiety of the sub- strates bind in a nonoptimal conformation. Another interesting feature concerning ... acids lining this space. Binding of BVDU to Dm-dNK-Y70W compen- sates for the loss of one hydrogen bond to 3¢-OH by a tighter fitting of the bromovinyl group, thus rest...

Ngày tải lên: 19/02/2014, 02:20

... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various DEC1 ... eletion of 111 amino acids f rom the N-terminal region (BMAL1:112–626) did not affect the binding of BMAL1 to DEC1; deletion of 235 amino acids (BMAL1:236–626) slightly dim...

Ngày tải lên: 19/02/2014, 16:20

... Llinas M (1988) Lysi- ne ⁄ fibrin binding sites of kringles modeled after the structure of kringle 1 of prothrombin. Proteins 3, 85–96. 20 Thorsen S (1975) Differences in the binding to fibrin of native ... contribute to this inhibitory effect. We then investigated the consequence of the inhibi- tion of plasminogen binding on plasmin formation by fibrin-bound tissue pla...

Ngày tải lên: 20/02/2014, 01:20

... [14,20]. Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the structure ... base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal...

Ngày tải lên: 20/02/2014, 11:20

... rates in the presence of kirromycin The effect of kirromycin on the growth of GRd.tsf was investigated by measuring growth rates of cultures in LB containing various concentrations of kirromycin. ... reaction in EF-Tu was investigated by studying the functional effects of deleting the motif in EF-Ts, both in vivo and in vitro. The motif was deleted i...

Ngày tải lên: 21/02/2014, 00:20

... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig. 2). PCR reactions performed in ... also the function of the DRTGG domain. Taking into account the important role of Ptas, the results obtained in the present work, dissecting the differen...

Ngày tải lên: 06/03/2014, 11:20