Báo cáo khóa học: Functional analyses of placental protein 13/galectin-13 pdf
Báo cáo khóa học: Functional analyses of placental protein 13/galectin-13 pdf
... of aggregation to syncytiotrophoblasts in the case of normal placental function, and plasma levels of PP13/galectin-13 did not correlate with the intensity of the placental synthesis of the protein, ... sequences of the close homologues. The order of the protein list was based upon their homology to PP13/galectin-13. sPP13, similar to placental protein 13; PP13LP, pl...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... mixture of 10 mL taken from each of the five reaction mixtures. The method consists of adding various iodoacetamide and iodoacetate ratios to portions of the protein to generate a complete spectrum of ... (1996) Assessment of th e ATP binding prop erties of Hsp90. J. Biol. Chem. 271, 10035–10041. 35. Quemeneur, E., Guthapfel, R. & Gueguen, P. (1994) A major phosphoprotein...
Ngày tải lên: 16/03/2014, 16:20
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... experiments. Protein- bound iron FereneS was added in a final concentration of 20 mm to the protein sample to give a total volume of 600 lL and a protein concentration in the range 5–10 lm. The formation of ... in the course of the reaction. The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c. This was vali- dated by adding a total...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... quantitation of microgram quantities of protein util- izing the principle of protein dye binding. Anal Biochem 72, 248–254. 22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B & Loffler M (1996) Functional ... binding site of herpes simplex type 1 thymi- dine kinase analyzed by X-ray crystallography. Proteins 41, 545–553. 20 Laemmli UK (1970) Cleavage of structural proteins du...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala did not affect the interaction of D EC1 w ... com- petition for binding to E-boxes and/or protein protein interactions of DECs with BMAL1 [ 7]. Furthermore, w e recently demonstrated the existence of a novel autofeedback...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
... equation that allows calculation of the concentration of mAb that produces 50% of the inhibitory effect (IC 50 ) [61]. Effect of anti-LBS mAbs on the activation of plasminogen by fibrin- and cell-bound ... proteins, and the reaction was started by adding 50 lL per well of plasminogen and of CBS0065 in the same buffer. Kinetics of plasmin formation was followed for 5 h by measur...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... important functional role, such as the correct positioning of the SAP motifs in relation to the rest of the protein as well as for the stability of the protein. The truncated Ydc2 mutant proteins ... interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein protein or/and protein DNA interactions. The crystal structure of Ydc2 shows that deletion...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... the dimerization of EF-Ts in the crystal. In the crystal of E. coli EF-Tu–EF-Ts, a quaternary complex, formed by two molecules of each of the elongation factors, is observed. The coiled-coil motifs of each of ... both groups of media. Therefore, the growth rate of GRd.tsf ranges from 70– 95% of that of UY211 (Fig. 3). The lowest growth rate of GRd.tsf (70%), relative t...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... Cleavage of structural proteins during the assembly of the head of bacteriophage T 4 . Nature 227, 680–685. 21 Davis BJ (1964) Disc electrophoresis. II. Method and application to human serum proteins. ... share homology with the C-terminal domain of PtaIIs. Thus, the active site of PtaIIs is probably located at the C-terminal end of the protein, and the role of the PtaII N-term...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... leads to a reassessment of the value of the repeti- tive region of FnBPA ⁄ FnBPB as an immunogen, and raises the possibility of utilizing these proteins as com- ponents of a future anti-S. aureus ... expresses a number of proteins that can bind specifically to Fn [19,20]. The prototype of this class of protein is Fn-binding pro- tein A (FnBPA) [21]. The structural characteris...
Ngày tải lên: 06/03/2014, 22:21