Báo cáo khoa học: Identiﬁcation of a copper-repressible C-type heme protein of Methylococcus capsulatus (Bath) A member of a novel group of the bacterial di-heme cytochrome c peroxidase family of proteins docx

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... a thermal heating block and cooling to 4 C at a rate of 2 C min–1.Name SequencessDNAS d(CATTCCCACCGGGACCACCAC)ssDNA L d(CATTCCCACCGGGACCACCACCATTCCCACCGGGACCACCAC)ETS-1 d(TCTCTCGGTGGCCGGGGCTCGTCGGGGTTTTGGGTCCGTCC)Htelo ... TCA TGG CGT CCA CGG ATT ACA G)and EWS reverse d(CGC TCG AGT CAC TAG TAG GGCCGA TCT CTG C) , for pGEX–EWS; EAD forward d(CGGAAT TCA TGG CGT CCA CGG ATT ACA G) and EADreverse d(CGC TCG AGT CAT ... d(CGC TCG AGT CAT CCG GAA AAT CCTCCA GAC T), for pGEX–EAD; RGG1 forward d(CGGAAT TCC CAG GAG AGA ACC GGA GCA T) andRGG1 reverse d(CGC TCG AGT CAA TCA AGA TCTGGT CCT TCA TCC ATG G), for pGEX–RGG1;...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... studyasd1 AAG CCG ATG ACA CCA ATT (asd sense) This studyasd2 GCA GGT TCA TAG TGC ATG (asd antisense) This studyKan RP GGT GCG ACA ATC TAT CGA (kanamycin sense) [19]Kan FP CTC ATC GAG CAT CAA ATG ... studyats1 GCT CAA TCC GTG ATG TGA (ats sense) This studyats2 CGA CTG CAC TGA TGA GCT (ats antisense) This studylg1 CTT CAA GCC ATG TCA AAG (lgt6 sense) This studylg2 CGA ATA ATC ATC ACA CTG ... studyb2S CTCGAG CTC TTA ATG TTG ATA GTA ATT GGT GTC A (lpxL antisense; SacI site underlined) This studyatr1 TGC TTG ATG AGC CTA CCA (atr sense) This studyatr2 TGC TGA TGA TGG CAA CTC (atr antisense)...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... ampliﬁcation for FISHRpCAbrF TAC AAG GAT GCC ATT AGC 613–630RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839RpCAtrFprobe TAC AAA GAT CCA ATC CAG C 616–634RpCAtrRprobe TAA GAT TAC CAG AAT TGC 844–861 a Primers ... sequencing of RpCAbrRpCAbrF TAC AAG GAT GCC ATT AGC 613–630RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839RpCAbrR2 AGA GCA GCA GAC CTT ACG 706–723RpCAbrR3 GTT ACT TCC GCA GCT AGG 466–483Probe ampliﬁcation ... quantitative PCRRpCAbrFq a TGG TTT CAC CCC GTC GAA 932–949RpCAbrRq a GGT CTG GTC TTT TCT CGC CAT A 966–987RpCAtrFq a GCC AGG TGT CGT CCT CGT T 710–728RpCAtrRq a TCA CAA ATG TCC AGT GCC AGT T 757–778Full-length...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... substances may be aggregates of proteins and polysaccharides, and may play importantroles in formation of the phases and ⁄ or morphologies of the crystals because they consist of acidic glycoproteinsand ... which may comprise proteins andheparan sulfate glycosaminoglycan chains, although the structures of the proteins and the glycosaminogly-can chains were not characterized. Although it hasbeen ... Murayama E, Ohira T, Takagi Y & Nagasa-wa H (2006) Localization and diurnal variations of car-bonic anhydrase mRNA expression in the inner ear of the rainbow trout Oncorhynchus mykiss. Comp...

Báo cáo khoa học: Identiﬁcation of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt

... CGGAAACGCCTTAAGTCCAG GCCACAATCCAGTCATTCCA 83MT 2A AATAAGCTTCCGACTCTAGCCGC GATAAGCTTGTGGAAGTCGCGT 259CD237904 AGCTGGTGCAGGAGGAAGTA TCTCACTGGCCCTAAACTGG 92AL707095 CCGAGAACCGAACTTACCAA CTGATAGGGGTTGGGTGATG ... CTGATAGGGGTTGGGTGATG 128AK095731 AGGAAGCACCCAGCAATACCA GCATTTCCATTTCCCTAAGCAC 109DKK1 CACCTTGGATGGGTATTCCA CAACACAATCCTGAGGCACA 114BC037851 CACAGCTCCCATTCATTCCA TCCCTTTGCCTCCTGTTGTT 107b-Actin TCCTCCCTGGAGAAGAGCTA ... (5¢-to3¢)Size(bp)MDR1 GAAGAAGGGCCAGACGC CTCCTGGGACACGATGC 178MRP1 CCTTCGCTGAGTTCCTGC CTGCGGTGCTGTTGTGG 246BCRP ACATCAGCGGATACTACAGAG CACCATCATAAGGGTAAACAT 173CA9 TTTGAATGGGCGAGTGATTG ACAGCAAAAAGGAGGCCAAA 138BMP2...

Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAAGGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGGTCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGTCATGACCTCG-3¢. One strand of each oligonucleotide ... (ACGCTCACTGGAACACTGGAATGCCCAGTTCTCGTCGCTCACTGGAACACTGGAATGCCCAGTTCTCGTTCGCTCACTGGAACACTGGAATGCCTCTAG) upstream of the thymidine-kinasepromoter of reporter plasmid pUTK-Luc vector, whichcontains a ﬁreﬂy luciferase ... 5¢-CCGTAAGGTCAAGGTCACTCG-3¢,W. Wu et al. S. mansoni NR1FEBS Journal 274 (2007) 390–405 ª 2006 The Authors Journal compilation ª 2006 FEBS 401DR1: 5¢-CCGTAAGGTCACAGGTCACTCG-3¢, DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢,...

Báo cáo khóa học: Identiﬁcation of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen ppt

... in the mammalian kidney [2,4,5] and plant roots [6,7]. The yeast Saccharomyces cerevisiae has four such MIP channels: the aquaporins Aqy1 and Aqy2 and the aquaglyceroporinsFps1 and Yﬂ054 [8]. Aqy1 ... (MIP) family] have been found in archea,eubacteria, fungi, plants, animals and human [2,3]. Aqua-porins facilitate the diffusion of water across biologicalmembranes while the closely related aquaglyceroporinsmediate ... Sweden;3Swansea Clinical School, University of Wales Swansea, UK;4School of Lifeand Health Sciences, Aston University, Birmingham, UKAquaporins and aquaglyceroporins mediate the transport of waterandsolutesacrossbiologicalmembranes.Saccharo-myces...

Báo cáo khoa học: Identiﬁcation of NF1 as a silencer protein of the human adenine nucleotide translocase-2 gene pptx

... )525(5¢-TGACCTTGTCTCGTTGCCTCACCC-3¢)and)378(5¢-GCTACAGGGATGCCAAAAGAACCC-3¢)fortheSite-3, and primer set )346 (5¢-GCGTCTCACCCTAGTCCTGGTCCTGC-3¢)and)214 (5¢-GGAAGGGGCGGGTCCAGAGAACA-3¢) for the Site-2 element. PCR wasperformed ... DNAsused in EMSA analysis were: NF1 wt, 5¢-TTTTGGATTGAAGCCAATATGATA-3¢;NF1mut,5¢-TTTTGGATTGAATAAAATATGATA-3¢;Site-2wt,5¢-GCGTCTCACCCTAGTCCTGGTCCTGCTCCAAGGGTTTTTGTCC-3¢;Site-2mut,5¢-GCGTCTCACCCTAGTAATGGTAATGCTCCAAGGGTTTTTGTCC-3¢;Site-3wt,5¢-GGGTTCTTTTGGCATCCCTGTAGC-3¢;Site-3mut,5¢-GGGTTCTTTTTAAATCCCTGTAGC-3¢.Chromatin ... 5¢-TTTTGGATTGAAGCCAATATGATA-3¢;NF1mut,5¢-TTTTGGATTGAATAAAATATGATA-3¢;Site-2wt,5¢-GCGTCTCACCCTAGTCCTGGTCCTGCTCCAAGGGTTTTTGTCC-3¢;Site-2mut,5¢-GCGTCTCACCCTAGTAATGGTAATGCTCCAAGGGTTTTTGTCC-3¢;Site-3wt,5¢-GGGTTCTTTTGGCATCCCTGTAGC-3¢;Site-3mut,5¢-GGGTTCTTTTTAAATCCCTGTAGC-3¢.Chromatin immunoprecipitationChromatin immunoprecipitation of...

Báo cáo khoa học: Identiﬁcation of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx

... of this novel structural feature identiﬁed a further degree of variability within the inner-core oligosac-charide of meningococcal LPS which may contribute to the interaction of meningococcal ... meningococcal clinical isolates revealed anadditional and uniquely located glucose residue in the coreoligosaccharide that had not previously been identiﬁed inmeningococcal LPS.Materials and ... conservedinner-core structure of meningococcal LPS has led to the utilization of mutants defective in the enzyme, resultingin truncation of the oligosaccharide chains of the LPS at the glucose...

Báo cáo khoa học: Identiﬁcation and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

... tttcagGATGTG6 75 ACCACGgtaggc 7814 aaccagGTTATT7 87 TTGCAAgtatgc 3811 tttcagACCCTA8 157 ACCAGGgtaagt 5461 atttagCTTCAT9 49 AACAAGgtaaga 2646 ctttagGGGAAA10 90 TACCAGgtatga 11341 atctagCATTGA11 ... 5¢-GTTCCTGAGCAAAGTCTTCAATG-3¢ and 5¢-GGAATTCAGTGGAAAAACTTTACAT-3¢ for XPR130, the primers5¢-GTTCCTGAGCAAAGTCTTCAATG-3¢ and 5¢-GGAATTCAGTGGAAAAACTTTACAT-3¢ for XN73, and the primers 5¢-GGAATTCATGCCGACCACAACCGTTTTAAG-3¢ ... performed, each time using a nested primer(5¢-ACACTCCTCACCACCAACTACC-3¢ for PCR 2 and5¢-CTCTCCACCTATTATTTTATGAGTAACATCC-3¢for PCR 3) and the anchor primer supplied in the 5¢-RACEkit. For ampliﬁcation...

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