Báo cáo khoa học: Deciphering the key residues in Plasmodium falciparum b-ketoacyl acyl carrier protein reductase responsible for interactions with Plasmodium falciparum acyl carrier protein pptx

... PfACP. Abbreviations ACP, acyl carrier protein; FabG, b-ketoacyl acyl carrier protein reductase; FAS, fatty acid synthase; PfACP, Plasmodium falciparum acyl carrier protein; PfFabG, Plasmodium falciparum b-ketoacyl acyl ... key residues in Plasmodium falciparum b-ketoacyl acyl carrier protein reductase responsible for interactions with...

Ngày tải lên: 23/03/2014, 10:20

... enhanced in the presence of acyl carrier pro- tein. Acyl carrier protein increases the accessibility of NADPH to b-keto- acyl- acyl carrier protein reductase, as evident from the increase in the accessibility ... acyl carrier protein; apo-PfACP, Plasmodium falciparum acyl carrier protein (apo form); holo-PfACP, Plasmodium falciparum acyl carri...

Ngày tải lên: 23/03/2014, 10:21

... prepared for the time-course assay. Stimulation of cells was performed at 10-min intervals for 400 min with IL-1a. After 400 min, the plates were inverted to remove media into a dish containing Virkon ... for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the affinity of the enzyme fo...

Ngày tải lên: 16/03/2014, 11:20

... 2009) doi:10.1111/j.1742-4658.2009.07272.x In searching for new targets for antimalarials we investigated the biosyn- thesis of hypusine present in eukaryotic initiation factor-5A (eIF-5A) in Plasmodium. Here, we describe the cloning ... synthesizing the chromophore, phycocyanobilin (PCB) and the His6-tagged acceptor proteins (CpcA or PecA) [4] Con- trols were the respect...

Ngày tải lên: 29/03/2014, 22:21

... of the dry weight in the WT mice and 26.4 ± 3.1% in the VLCAD KO mice – under nonfasting conditions, and these percentages increased further with fasting (Fig. 1). In parallel with liver lipids, ... mice fed with the MCT diet presented significantly higher catalase activity in the nonfasting state than VLCAD KO mice fed with the LCT diet. Fasting further increased c...

Ngày tải lên: 15/03/2014, 23:20

... fresh medium containing inhibitor and A23187 (5 l M), and the cells were further incubated for 10 min. The medium was collected for the mea- surement of PGF 2a and PGE 2 levels by performing the respective ... the downstream signaling pathways including the Gq heterotrimeric G protein, and then further increases the intracellular calcium level and activates various kinases...

Ngày tải lên: 28/03/2014, 22:20

... crystal- line form, integrated within the full length gpE [24,25]. Contribution of the CDR3 loops to the energy of interaction The CDR3 loops of mAb4E11 comprise nine residues for the V L domain and ... antibodies contain six hypervariable regions in the variable (V) domains, three in the heavy chain V H and three in the light chain V L , that determine the complem...

Ngày tải lên: 19/02/2014, 07:20

... The conserved tyrosine residues 401 and 1044 in ATP sites of human P-glycoprotein are critical for ATP binding and hydrolysis: evidence for a conserved subdomain, the A-loop in the ATP-binding cassette. ... Vi for trapping is almost normal. By contrast, when the charge is removed, as in the glutamine (length of the side chain maintained) and asparagine (shorter side...

Ngày tải lên: 07/03/2014, 06:20

... to introduce electrostatic interactions with other nearby lysine residues. This, in turn, is likely to reduce the flexibility of the section linking the N-terminal regulatory domain with TMD1, thereby ... mutations in 14 distinct resi- dues, all facing the inside of the cell. Five of the residues lie within the previously characterized N-terminal regulatory domain an...

Ngày tải lên: 07/03/2014, 15:20

... gorge with the possibility of multiple binding sites for each form. However, in all these models, the benzyl group interacts with Trp84 at the bottom of the gorge, the piperidine ring interacts with ... inhibitors have indicated that both the benzoyl containing functionality and the N-benzylpiperidine moiety are the key features for binding and inhibition of AChE...

Ngày tải lên: 30/03/2014, 20:20