Báo cáo khoa học: Leishmania infantum LeIF protein is an ATP-dependent RNA helicase and an eIF4A-like factor that inhibits translation in yeast docx

... protein is an ATP-dependent RNA helicase and an eIF4A-like factor that inhibits translation in yeast Mourad Barhoumi 1 , N. K. Tanner 2 , Josette Banroques 2,3 , Patrick Linder 2 and Ikram Guizani 1 1 ... purified, recombinant, LeIF protein from Leishma- nia infantum, and we demonstrate that it is an RNA- dependent ATPase and an ATP-dependent...

Ngày tải lên: 23/03/2014, 10:20

... fluorescent protein, to the protein of interest [10]. Fluorescent protein domains may interfere with protein folding or ligand binding and, more particularly, with protein packing into ordered arrays. ... RE, Gross LA, Martin BR, Walkup GK, Yao Y, Llopis J & Tsien RY (2002) New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synth...

Ngày tải lên: 06/03/2014, 11:20

... potentials that are indistinguish- able from that of the mother cell. This process is cen- tral to development, as well as to the maintenance of adult tissues in complex and long-lived organisms. Self-renewal ... roles in RNA processing, including the splic- ing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site-dependent translation. Al...

Ngày tải lên: 07/03/2014, 00:20

... cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue. Am J Pathol 166 , 303– 312. 53 Grounds MD & Torrisi J (2004) Anti-TNFalpha ... a role. In particular, cardiac ankyrin repeat protein (CARP) was suggested to be involved in these pro- cesses. CARP, together with ankyrin repeat domain- containing pr...

Ngày tải lên: 07/03/2014, 03:20

... scaling factor determined by Fourier self-deconvolving the spectra, deter- mining the tyrosine band a rea ratios at 1518 cm À1 ,andthen using this as the scaling factor for subtracting the original spectra ... data points in order to bring the minimum to zero. Combination of CD and IR spectra To analyze combined C D and IR data, h ybrid spectra were made by placing CD and IR data...

Ngày tải lên: 16/03/2014, 18:20

... capture ligand for sEPCR and protein C is illustrated in Fig. 2C. This shows ini- tial binding of sEPCR to RCR-2 (Fig. 2C; panel 1), the addition of increasing concentrations of protein C – showing ... apoptosis in ischemic human brain endothelium and is neuroprotec- tive. Nat Med 9, 338–342. 17 Bangalore N, Drohan WN & Orthner CL (1994) High affinity binding sites for acti...

Ngày tải lên: 16/03/2014, 18:20

... needed. Zinc is an essential metal in all eukaryotes. Zinc transporting proteins were first reported in yeast and plants. In mammals, the ZIP superfamily is the most studied zinc transporter. Human zip1 and ... nine transmembrane domains (according to Taylor et al. [15] and five transmembrane prediction software packages), histidine-rich motif [(HX)n, n ¼ 3–6], LZT protein...

Ngày tải lên: 30/03/2014, 16:20

... of long-chain acyl-CoA esters in signal- transduction pathways is an important issue [25]. It is known that an acyl-CoA-binding protein known also as DBI (diazepam-binding inhibitor) is expressed ... mitochondrial cristae [17]. In accord- ance with the postulated role of MTE-I in steroido- genesis, we detected the protein and its mRNA in the adrenal gland, ovary, tes...

Ngày tải lên: 16/03/2014, 18:20

... PDZ proteins: Mint-3, AMPA binding protein and gluta- mate receptor interacting protein (GRIP). It would therefore be interesting to determine whether this motif is also involved in the interaction ... cells transfected with pCDNA3.1 Zeo+ and MT1/ MYC (lane 1), pCDNA3.1 Zeo+ and GRASP55F (lane 2), pCDNA3.1 Zeo+ and MT1 LLY/MYC (lane 3), GRASP55F and MT1 LLY/MYC (lane 4) and...

Ngày tải lên: 18/02/2014, 04:20

... There is evidence that the heteromeric guanine nucleotide- binding regulatory protein (G protein) is also involved in TLR4 activation. It has been shown that LPS induced TNFa production which can ... their inactive conforma- tion G proteins consist of a-, b- and c subunits, whereas only the a subunit is bound to GDP. GPCR are transmembrane receptor proteins, containing seven...

Ngày tải lên: 18/02/2014, 13:20