... sequencing was performed (Mac-rogen Inc., Seoul, South Korea) using standard protocols[20]. Expression in E. coli and preparation of crude cellularextracts Expression was carried out in E. ... lLof1mgÆmL)12,2¢-azinobis(3-ethylbenzo-6-thiazolinesulfonic acid), pre-paredin20mMcitrate/80 mMNa2HPO4, pH 4.3, contain-ing 1 lLÆmL)130% H2O2,wasaddedtoeachwellandincubated f or 10 min in t ... recombinant proteinwas deduced. The amino-acid sequence was found to beidentical with the 3D7 strain. However, these two strainsdiffer from the K1 strain at position 131 in having analanine...