Báo cáo khoa học: Differential susceptibility of Plasmodium falciparum versus yeast and mammalian enolases to dissociation into active monomers doc

... report successful dissociation of dimeric enolases from Plas- modium falciparum, yeast and rabbit muscle into active and isolatable monomers. Dimeric enolases could be dissociated into monomers by ... falciparum versus yeast and mammalian enolases to dissociation into active monomers Ipsita Pal-Bhowmick, Sadagopan Krishnan and Gotam K. Jarori De...

Ngày tải lên: 23/03/2014, 09:20

... interacting factors could modify the molecular phenotypic effect of using this vector. This is the first report of the detection at the molecular level of the toxic effect of a DNA vector in P. falciparum. Although ... Luzzatto L & Mason PJ (2003) Expression of Plasmodium falciparum G6PD-6PGL in laboratory parasites and in patient iso- lates in G6PD-deficient and normal...

Ngày tải lên: 19/02/2014, 07:20

... science divi- sion of NOW, and the technology programme of the Ministry of Economic Affairs. F. Meersman is a post- doctoral fellow of the Research Foundation Flanders (FWO-Vlaanderen). References 1 ... inactivation of b-glucosidase from P. furiosus was studied at pressures up to 900 MPa, at temperatures of 25, 40 and 60 °C, and at pH 5.5, 6.0 and 6.5. Gel electrophoresi...

Ngày tải lên: 07/03/2014, 03:20

... sequence of P. falciparum ICDH shows a high degree of identity to the mitochondrial NADP + -dependent ICDH of mammals (55.3% to 57.7%) and yeast (46.6%) (Fig. 1) but has only little identity to E. ... is found in the cytosol and nucleus of the cell [46]. Fig. 5. Expression levels of ICDH in oxidatively stressed P. falciparum. (A) Northern blot analysis of P. falcip...

Ngày tải lên: 08/03/2014, 02:20

... irect and receptor- mediated activation of PKC in a cellular model of downregulation o f PKCe and/ or PKCa to understand the respective roles of these specific isoforms of PKC in the activation of ... MM05221899–2000 to S. G.), from the University of Pavia ( FAR 2003 to M. R .; Progetto Giovani Rice rcatori to M. M., C. L. and E. P.) a nd from the Italian Ministry of...

Ngày tải lên: 23/03/2014, 13:20

... number of substitu- tions. The high conservation of the active site suggests that inhibitors specific to the P. falciparum active site will be difficult to find. Thus, an alternative means of inhibition ... protocols were used during the production runs. In the first protocol, a Langevin piston was used to maintain pressure at one atmosphere (NP ensemble) with a piston period o...

Ngày tải lên: 29/03/2014, 23:20

... organized into an a and an a ⁄ b domain, and the active site is located in a positively charged cleft between the domains. Three sulfate ions bound to the catalytic pocket of an inactive mutant ... Comparison with the structures of the related enzymes AppA and G1P of E. coli suggests the existence of a common ancestor (‘prototype’) of HAPs, endowed with the potential to...

Ngày tải lên: 16/02/2014, 09:20

... upstream of the start codon of YEF1 (Table 5). The use of pET-DEST42 enabled us to fuse a V5 epitope and a His 6 tag to the C-terminal of Yef1p. E. coli BL21(DE3) was transformed with pET-YEF1 and ... respectively. K m and V max for NAD and NADH of Pos5p have not been reported [2,3]. Constructions of double and triple mutants for UTR1, YEF1 and POS5 To examine th...

Ngày tải lên: 20/02/2014, 01:20

... C, Plumpton C, Romanos MA & Dowell SJ (2000) Func- tional coupling of mammalian receptors to the yeast mating pathway using novel yeast ⁄ mammalian G pro- tein alpha-subunit chimeras. Yeast ... Expression of olfactory receptors in yeast for screening FEBS Journal 272 (2005) 524–537 ª 2004 FEBS 537 Functional expression of olfactory receptors in yeast and developmen...

Ngày tải lên: 07/03/2014, 16:20

... versions of these two isoforms, as well as of the Mxi1-WR isoform that lacks the SID, and shown to give rise to proteins of the expected size expressed at similar levels (Fig. 1A). Each of these ... on Mxi1-SRa contributes to the differential func- tions of Mxi1-SRa and Mxi1-SRb in Myc antagonism. Deletion of this domain converts Mxi1-SRa into a potent suppressor of...

Ngày tải lên: 18/02/2014, 16:20