Báo cáo khoa học: Vps4 regulates a subset of protein interactions at the multivesicular endosome doc

... GCCCATATTCGTCGACGCGCTAACAGGTACCAGAGGAGAAGGAGAGAGCGAAGCAAGTAG Vps4 Dstr R GGGCGGATCCTCTGCTTTTCTTTATC YEE F CTGGACACAGCCACGCAGTATACAGCATACTATAACGG YEE R CCGTTATAGTATGCTGTATACTGCGTGGCTGTGTCCAG IRA F CCTAAGTCGAAGGATTTGAAGCACTTGGAAAGTGAAG IRA ... Table 2. Primers used for mutagenesis. Primer Sequence (5¢-to3¢) Vps4 Upstr F CGCTGCAGTAAGAGCAGTAAACCCG Vps4 SalIR GAGAATCAGTGTCGACTTCATCTATAAAAATA...

Ngày tải lên: 23/03/2014, 09:20

... TTCTTAAAACTTGACCAAA Primer 2 GGGTACGATGACTAAATGATCGGA Primer 3 (Figs 3 and 5A, B) TCATCAGCAGCCCTTCTTTGCACAAC Primer 3 (Figs 4 and 5C) GACTAAATGATCGGATACGTGCCCATTCT Lower strand Primer 1 CTCAACGTCATTCTAAAGT Primer ... CTCAACGTCATTCTAAAGT Primer 2 ACAACATACTTCGAAACTGTGACC Primer 3 TGTCCTGGCACATGACCCGGATCA Transcription factors at the CPS GRU M. Hoogenkamp et al. 44 FEBS Journal 274 (2007) 3...

Ngày tải lên: 23/03/2014, 10:20

... Petersburg, FL, USA) followed by PCR with Taq DNA polymerase (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The cDNA was subcloned ... that mammalian Gup1, a mem- ber of the MBOAT superfamily bearing sequence simi- larity to HHAT, acts as a negative regulator of N-terminal palmitoylation of Shh. Se...

Ngày tải lên: 18/02/2014, 16:20

... is the size of the vocabulary and K is the number of clusters. The hierarchical nature of the clustering means that we can choose the word class at several levels in the hierarchy, which can ... fast, testing is still expensive when the size of the vocabulary is large. Instead of cross-validating using the log-rank over the validation data as they do, we inst...

Ngày tải lên: 20/02/2014, 04:20

... annotated instances. In contrast, our study focused on a se- lect group of nominal predicates, each associated with a large number of annotated instances. 3 Data annotation and analysis 3.1 Data annotation Implicit ... undergraduate assistant’s annota- tions against the evaluation data. Our assistant achieved an overall F 1 score of 58.4% using the same candidate window as the...

Ngày tải lên: 17/03/2014, 00:20

... encompasses linguistic services that are being developed, in all or part, and main- tained at the University of Lisbon, Department of Informatics, by the NLX-Natural Language and Speech Group. At ... demonstrat- ing a range of language technology tools and at fostering the education, research and development in natural language sci- ence and technology. 1 Introduction This...

Ngày tải lên: 17/03/2014, 02:20

... gagt … tcac ag GGATAT 1.8 3 105 CAACAG gt aata … ttcc ag ACGTAT 7.7 4 262 a TACTTG gt atgt … aatc ag GATATG 1.3 5 120 a ACAGAG gt aaaa … tctc ag AAAATT 9.9 6 138 GCATTG gt aagg … attt ag GGCAGT ... 1024 AAGGAC gt aagt … ttca ag GATTGC 0.57 11 176 AGAAGA gt aagt … ttgc ag CAATTT 4.5 12 130 ATGTTG gt gagt … tttt ag GGCATA 3.9 13 85 ACTCAA gt aagg … taat ag GATTTC 1.1 14 51 ATGTAG gt aagt …...

Ngày tải lên: 23/03/2014, 13:20

... are unclear. The first reaction of the CDP-ethanolamine branch of the Kennedy pathway is catalysed by etha- nolamine kinase, resulting in the formation of phosphoethanolamine, which in turn is activated ... [64]. The contribution of the PS decarboxylation reaction and the CDP-ethanolamine branch of the Kennedy pathway to PE formation in mammalian cells has been experi...

Ngày tải lên: 28/03/2014, 23:20

... M, Kaneko S, Kuno A, Koyama Y, Yoshida S, Park GG, Sakakibara Y, Kusakabe I & Kobayashi H (2001) Purification and characterization of the recom- binant Thermus sp. strain T2 a- galactosidase ... Transferase action of a- galactosidase from tubers of Stachys affinis. Agric Biol Chem 46, 1089–1090. 31 Hashimoto H, Katayama C, Goto M, Okinaga T & Kitahata S (1995) Transglycosylat...

Ngày tải lên: 29/03/2014, 21:20

... undergo passivization. 1 Linguists mainly attribute this to the fact that only a referential noun can appear as the surface subject of a passive construction. 1 There are idiomatic combinations that are ... lexically fixed to the extent that its deviates from the average of its vari- ants. Our measure calculates this deviation, nor- malized using the sample’s standard deviati...

Ngày tải lên: 31/03/2014, 20:20