Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt

... 6074–6084 ª 2007 The Authors Journal compilation ª 2007 FEBS Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation Min-Sun Kang 1 , Masayuki Okuyama 1 , Katsuro ... 2007) doi:10.1111/j.1742-4658.2007.06129.x The specificity of the aglycone- binding site of Escherichia coli a-xylosidase (YicI), which belongs to glycoside hydrolase...

Ngày tải lên: 23/03/2014, 07:20

... regardless of the presence of uridine at other positions in the loop of helix 69 of 23S rRNA variants; (b) substitution of one U by C has no effect on the conversion of others (i.e. formation of pseudouridines ... and U1917 of E. coli 23S rRNA, at least in vivo. Mutations at position A1916 affect the specificity of RluD Substitution of uridine by cytidine at positio...

Ngày tải lên: 18/02/2014, 16:20

... substrates of the two peptides is due to different positions being modified by the enzyme. Our data indicate a site specificity of the yeast HAT-B complex that exactly matches the specificity of other type ... fractionation of HAT enzymes were obtained by lysis of yeast spheroplasts in a low-salt medium. Briefly, yeast cells growing exponentially, or after 3 h of incubation wi...

Ngày tải lên: 07/03/2014, 05:20

... model of VVTMP kinase, built by homology with the human liganded enzyme and proposed by SwissProt contains the structural features of the human enzyme: a five par- allel b-strand core surrounded by ... obtained by fitting are 17 (±3), 25 (±2) and 21 (±1) l M, respect- ively. (C) Phosphate donor analysis of VVTMP kinase. The activity is expressed as the rate of phosphorylation o...

Ngày tải lên: 16/03/2014, 14:20

... the specificity of this isoform. The aim of this study was to identify amino acid res- idues that are involved in substrate specificity of UGTs 2B4 and 2B7 in order to better understand the molecular basis of ... for substrate specificity of the enzyme towards HDCA. Investigation of the deficiency in HDCA glucuronidation by the UGT2B4 variant described by Jin et al. [11] led...

Ngày tải lên: 23/03/2014, 09:21

... reported by Deltour et al. [29]. Full-length cDNAs were sequenced by Oswel Research Products Ltd (University of Southampton, UK). Expression and purification of ADH proteins Escherichia coli BL21 ... Merck). Negative immunostaining controls were made by the preadsorption of the ADH4 antibody with an excess of purified recombinant ADH4, or by the omission of the primary ant...

Ngày tải lên: 23/03/2014, 12:20

... enzymes. Fig. 4. Time-course of cleavage of modified DNA duplexes I–III by the formamidopyrimidine-DNA glycosylase (Fpg protein). The extent of cleavage was determined by PAGE as the ratio of the incised DNA radioactivity ... indicate that introduction of the TFMDPh group in close proximity to the oxoG residue has no effect on the recognition and binding of DNA duplexes by th...

Ngày tải lên: 20/02/2014, 11:20

... thank DST, Government of India, and MHRD, Government of India, for financial support. We also thank Professor Winfried Boos, University of Konstanz, Germany, for the gift of plasmids encoding His-tagged ... W (1991) The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase. J Biol Chem 266, 19450–19458. Chaperone-assisted folding of...

Ngày tải lên: 16/03/2014, 06:20

... mechanism of pyrimidine dimer repair by DNA photolyase. Photochem Photobiol 57, 895–904. 28 Hamm-Alvarez S, Sancar A & Rajagopalan KV (1990) The folate cofactor of Escherichia coli DNA photolyase acts ... at an elucidation of a detailed molecular picture of the light-driven DNA repair process. Results Construction of a DNA photolyase E109A mutant MTHF, the second cofactor...

Ngày tải lên: 16/03/2014, 18:20

... Physico-Chemical Biology and School of Chemistry, Moscow State University, Moscow, Russia; 2 Department of Biochemistry, University of Turku, Finland We report here that Escherichia coli pyrophosphatase aggregates ... followed by measuring the absorbance of enzyme solution at 440 nm in a quartz cuvette of 1 cm path length. In kinetic experiments, an aliquot of 0.1 M cadmium...

Ngày tải lên: 23/03/2014, 13:20