Báo cáo khoa học: Recruitment of coregulator complexes to the b-globin gene locus by TFII-I and upstream stimulatory factor doc

Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

... analyzed recruitment of factors to the a-globin gene locus dur-ing the differentiation of erythroid cells. The regula-tory elements located upstream of the a-globin genesalso appear to initiate the ... activation of the gene locus. However, in contrast to the b-globin LCR and the VpreB1 and k5 gene locus, the a-globin regulatory ele-ments do not recruit RNA Pol II and it appears that recruitment of ... appear to spread outward in both directions to the genes it controls. This is identical to the observed appearance of these marks at the LCR of the globin locus in ES cells followed by the genes...

Báo cáo khoa học: Recruitment of coregulator complexes to the b-globin gene locus by TFII-I and upstream stimulatory factor doc

... Recruitment of coregulator complexes to the b-globin gene locus by TFII-I and upstream stimulatory factor Valerie J. Crusselle-Davis, Zhuo Zhou, Archana ... to the b-globin gene. Together,these data demonstrate that the antagonistic activities of upstream stimula-tory factor and TFII-I on b-globin gene expression are mediated at least inpart by ... for the recruitment of CBP ⁄ p300 to both the b-globin gene and the LCR.Expression of the dominant negative mutant alsoreduced interactions of RNA polymerase II with LCRHS2 and the b-globin gene...

Báo cáo khoa học: Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco pot

... information), and the 5¢-termini of the NtFAD3transgene cDNAs were distributed in the same region of the transgene sequences, suggesting that the NtFAD3transgene was transcribed under the control of the same ... for the endogene). In contrast, the level of nuclear transcripts of the NtFAD3 transgene wasreduced to 0.75 ± 0.20 of those of S44 plants(mean ± SD, n = 3). We also analyzed TSP of the transgene ... Degree of cytosine methylation at the END1 region of the El2 promoter. (B) Degree of cytosine methylation at the END2 region of the El2 promoter. (C) Degree of cytosine methyla-tion at the NtFAD3...

Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

... available. To analyse further the composition of the PS1 complex,samples with high expression of PS1 complex were selectedfor further study. BN/PAGE gel bands corresponding to the region of PS1 complex ... [19] to analyse further and characterize endo-genous native complexes from mouse and human braintissue and from the neuroblastoma line SH-SY5Y (SY5Y).Experimental proceduresAntibodiesSynthetic ... Abpeptide) and a cytoplasmic fragment such as the APPintracellular domain or the Notch intracellular domainwhich translocates to the nucleus for regulation of gene expression [1]. The PS proteins...

Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate speciﬁcity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx

... binding to the catalytic domains of PAH and THDocking of the native cosubstrate BH4into the crystalstructure of the PAH catalytic domain yielded a total of 286 conformations. Out of these, ... displayed the expected coordination of the tyrosine aromatic ring towards the iron atom. However, the hydroxyl group of the aromatic ring was placed trans to His290. In contrast to the anchoring of the ... crystallography[14]. The distance between the C4a-Atom of BH4 and the iron was 5.97 A˚, thus closer to the value of 6.06 A˚in the crystal structure [14] than to the distance of 4.3 A˚measured by NMR...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... hand-offsoffset the reduced errors associated with better-rested careproviders? As noted by the authors, their intervention tookplace in a system that was designed to minimize the impact of ... calculated to be 80%, not 90%.In addition, there is one error in the description of the limitations that we would like to point out. Dr Sarani and DrAlarcon note:“There were more patients admitted to ... duty-hour standards:“Based on the results of these studies, it seems that the ACGME resident work hour restrictions are warranted, atleast for interns, and that efforts to reduce the number of hours...

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... coadministered (Fig. 4C).Taken together, these results indicate that the location of the protective action of oleate was upstream of ERstress activation.Whether the protection offered by oleate was speciﬁcfor ... Interestingly, the interruption of TAG synthe-sis preceded the appearance of toxic effects, supporting the notion that it constitutes the initiating event in the process of lipotoxicity.Stearate has to ... or a combination of the two (SA ⁄ OA) for 36 h.(C) The intensity of phosphorylation of JNK (p-JNK) and the amount of the total protein was examined by western blot analysis in totalcell extracts...

Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

... to onlyone acceptor molecule until the release of the ﬁnalproduct. Another mechanism is suggested by Mooseret al. on the basis of the identiﬁcation of the active site and the similarity with ... addition, the kinetics of the acceptor reactions appeared to be similar betweenGS and GSGB.Kinetic simulation of GTF glucan synthesis To explain the time course of saccharide productionduring the ... of the experimentaldata, even in terms of the total concentration of soluble nigerooligosaccharides (DP = 2–6). In otherwords, the difference between the total concentrations of fructose and...

Tài liệu Báo cáo khoa học: Use of hydrostatic pressure to produce ‘native’ monomers of yeast enolase ppt

... helices in the large domain and two b-strands in the small domain. The active site is at the bottom of the barrel and is totallyCorrespondence to M. J. Kornblatt, Department of Chemistry and Biochemistry, ... 251, 775–780]. The UV spectra of the high pressure forms of the Mg2+- and apo-forms of enolase are identical and distinctfrom the spectrum of the form obtained in the presence of 1mMMn2+; ... showsthat the spectra of the apo- and Mg2+forms of enolase athigh pressure are identical and differ from that of the Mn2+enzyme. As the spectrum of Mn2+-enolase differs from the other two...

Tài liệu Báo cáo khoa học: Cleavage of nonphenolic b-1 diarylpropane lignin model dimers by manganese peroxidase from Phanerochaete chrysosporium Evidence for a hydrogen abstraction mechanism docx

... 4-methoxyacetophenone (III)(Table 2). The percentage of 18O incorporated was estima-ted by the ratio of the M+/(M++2)peaksinthemassspectrum of the products. Neither the diarylpropane (I) nor the ... detectable amount of the a-keto diarylpropane (X) was produced in the absence of Tween 80 (Table 1). To determine possible pathways for the oxidation of the diarylpropanes, the oxidation of the intermediate ... about 30% of the diarylpropane VI was oxidized in the presence of Tween 80 during the 12 h incubation, in the absence of Tween 80, only about 5% of the VI was oxidized. The products of the oxidation...

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