Báo cáo khoa học: Bioenergetic requirements of a Tat-dependent substrate in the halophilic archaeon Haloarcula hispanica pdf

... hispa- nica was amplified using pET-AmyH as template, with primers AmyFor-NdeI (TTTGTTTAACTTTAAGAAGG AGATATA CATATGAATCG) and AmyRev-NcoI (aaaac catGGGCTTTGTTAGCAGCCGGAT). The amplified fragment was ... appears to play a dominant role in protein translocation in halophilic archaea (halo- archaea) [4,5]. These organisms live in concentrated brine, with the main salt usually being N...

Ngày tải lên: 23/03/2014, 06:20

... interaction of SRP pathway components in Archaea, the membrane binding of SRP54 and FtsY in the halophilic archaea Haloferax volcanii was investigated. Such studies offer insight not only into the ... densitometrically quantitated. Values shown represent the average values obtained ± SEM in the top and bottom halves of the gradients. Fig. 3. Haloarchaeal FtsY A dom...

Ngày tải lên: 16/03/2014, 16:20

... considered as follows. The role and importance of the aspartate in the catalytic triad is not fully understood because several serine proteases do not have an aspartate as the catalytic apparatus. However, ... mutants W169F–H21 0A and W16 9A H21 0A were constructed. Peptidase activity was determined using VLK-MCA as the substrate and the increase in fluorescence of the...

Ngày tải lên: 21/02/2014, 03:20

... 5¢-TATA CTCGAGAT GTCACCTTTAAGGCAGAG-3¢ and 5 ¢-ATAT CCATG GTCTCATCCATTCCTGCATAC-3¢. Abz1(45–138): 5¢- TATA CTCGAGATGCAGAAGCTTCTGCAAGATTT GAC-3¢ and 5¢-ATAT CCATGGAAAATTTAAACAA TCCTGATG-3¢. The ... 5¢-TAT GGATCCATGC TTCTGCAAGATTTGACAGG-3¢ and 5¢-ATAT CCC GGGTTAAAATTTAAACAATCCTG-3¢.C-terminal deletion, ABZ1(1–100): 5¢-TATA GGATCCATGTCACC TTTAAGGCAGAG-3¢ and 5¢ -A TA CCCGGGTTATAA ATACCTGAGCCT...

Ngày tải lên: 23/03/2014, 13:20

... Biosci- ence, San Jose, CA, USA). The concentration of the purified protein was determined by quantification of the intensity for the separated bands in SDS ⁄ PAGE analysis using imaging software ( multigauge ... substrate, we mea- sured the amount of enzymatic incorporation of the primary amine, monodansylcadaverine (Dansyl-Cd), in recombinant peptide-GST(QN) fusion pr...

Ngày tải lên: 29/03/2014, 21:20

... CGA TCA GCT TAC CGG GCT CTT G 3¢ C AAG AGC CCG GTA AGC TGA TCG GAA AGA AGG C TEII srf D18 9A 5¢ C TTT AAC GGG CTT GCT GAT AAA AAA TGC ATA CGA GAT GCG G 3¢ C CGC ATC TCG TAT GCA TTT TTT ATC AGC AAG ... AAG CCC GTT AAA G TEII srf D19 0A 5¢ C TTT AAC GGG CTT GAT GCT AAA AAA TGC ATA CGA GAT GCG G 3¢ C CGC ATC TCG TAT GCA TTT TTT AGC ATC AAG CCC GTT AAA G TEII srf 5¢ C TTT AAC GGG CTT GCT GCT AA...

Ngày tải lên: 30/03/2014, 13:20

... metazoa. Considering that IVD acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the investigation of ... not show any signs of developmental abnormality until the onset of spinning (Fig. 1A) . The mutants start spinning after the normal duration of the final instar (6...

Ngày tải lên: 18/02/2014, 04:20

... Tsubaki T, Takegawa S, Hanamoto H, Arita N, Kamogawa J, Yamamoto H, Takubo N, Nakata S, Yamada K, Yamamoto S et al. (2005) Accumulation of plasma cells expressing CXCR3 in the synovial sublin- ing ... in am- matory disease that occurs in about 1% of the popula- tion. The in ammatory process is characterized by in ltration of in ammatory cells into the joints, leading to...

Ngày tải lên: 18/02/2014, 18:20

... antisera and antibody. Antisera against annexin A1 , annexin A2 and annexin A5 were raised in both rabbit and mouse, and antiserum against dicalcin was raised in rabbit. G olf antibody raised in rabbit ... we obtained recombinant annexin A1 , annexin A2 and annexin A5 expressed in Escherichia coli. The apparent molecular masses of recombinant annexin A1 and ann- exin A2 w...

Ngày tải lên: 07/03/2014, 05:20

... pl2. 33 Nagano A, Koga R, Ogawa M, Kurano Y, Kawada J, Okada R, Hayashi YK, Tsukahara T & Arahata K (1996) Emerin deficiency at the nuclear membrane in patients with Emery–Dreifuss muscular dystrophy. ... characterized by a mislocalization of emerin that is caused by the loss of emerin binding to lamin A (X- linked form) or by the loss of lamin A (autosomal dominant fo...

Ngày tải lên: 07/03/2014, 12:20