Báo cáo khoa học: Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen doc

... strain did not show accumulation of the precursor of SufI (pre-SufI) (Fig. 4A, lane 4), Fig. 3. Photo-crosslinking of TF to the signal peptide of nascent SufI chains of various lengths. In vitro ... effect of increased i ntracellular TF levels on the efficiency of Tat-mediated export, steady state signals and export kinetics of endogeneous SufI were monitored upon overproductio...

Ngày tải lên: 07/03/2014, 16:20

... immunoblotting using antibodies directed against the His-tag for any of the combinations, which may be due to low expression levels or to processing of the His-tag. The lack of detection of both the con- structs ... complex of cit- rate with Ca 2+ [7]. The physiological function of BsCitH is unknown. The CitMHS family of transport- ers contains over 60 members, all of ba...

Ngày tải lên: 23/03/2014, 10:20

... domain antibodies FEBS Journal 276 (2009) 3881–3893 ª 2009 The Authors Journal compilation ª 2009 FEBS 3893 Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic ... isolated from an immunized llama several high-affinity sdAbs directed against human carcinoembryonic antigen (CEA), a heavily glycosylated...

Ngày tải lên: 23/03/2014, 05:22

... kinetic stability of R242A mutant of StP, compared to wild-type enzyme. It also provides a rational for an allosteric mechanism of stabilization of StP by oxyanion binding. In light of the fact that ... Nidetzky 1,3 1 Institute of Food Technology and 2 Centre of Applied Genetics, University of Agricultural Sciences, Vienna, Austria; 3 Institute of Biotechnology, Graz Univers...

Ngày tải lên: 31/03/2014, 01:20

... Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake counts in the presence of cocaine or S-cita- lopram, ... microtiter plates in a final volume of 200 lL of complete cell culture medium. After 48 h, the cells were incubated in the absence or presence of 0.5 mm DA. After 24 h of in...

Ngày tải lên: 14/02/2014, 21:20

... structure of C24. Doc. S1b. sbml format of model structure of C24. Doc. S2a. Model structure of Rsch. Doc. S2b. sbml format of model structure of Rsch. Doc. S3. Documentation of the model structure. This ... days of exposure to 4 °C. Samples were taken after a light period of 8 h. At that stage, the aerial part of the plant is exclusively composed of rosette leaves,...

Ngày tải lên: 14/02/2014, 22:20

... Moreover, endothelial cells from tissue segments of internal mammary arteries from smokers with coronary artery disease express higher levels of ANGPTL2 mRNA than tissues from non- smokers with similar ... (No.22117514) from the Ministry of Education, Culture, Sports, Sci- ence and Technology of Japan, by Grants-in-Aid for Scientific Research (B) (No. 21390245) from Japan Soc...

Ngày tải lên: 14/02/2014, 22:20

... chan- nel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues from the SET-C and post-SET regions. Lysine 4 of histone H3 is inserted ... investigation by Patel et al. [35], MALDI-TOF MS was used to quantitate the degree of H3K4 meth- ylation, which shows an accumulation of the dimethyl from of H3K4 wi...

Ngày tải lên: 16/02/2014, 14:20

... role of GIF in neurofibrillary changes associated with AD. Potential role of metal-binding/exchange properties of GIF in AD One of the primary pathological hallmarks of AD is the formation of b-amyloid ... the absence of brain extract (either AD or from the nor- mal brain) GIF promotes neuron survival of cultured cortical neurons [31]. The neuroinhibitory action of GIF is v...

Ngày tải lên: 16/02/2014, 15:20

... the exception of E95, a stretch of four occluding loop resi- dues from V112 to S115 in the first molecule of cathepsin B; G75 and Q76 in the molecule A of stefin A; and M1 and E78 in the molecule B of stefin ... the back of the molecule, whereas the side chain of H111 points upwards and away from the surface. In the complex, two stefin A residues, A49 from the tip of the firs...

Ngày tải lên: 18/02/2014, 04:20