Báo cáo khoa học: Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma docx

... significantly higher in CCA cell lines than non-CCA-type EOC cell lines (Fig. 5A). In addi- tion, in other cell lines carrying the wild-type p53 gene, HEK293 or LNCaP cell lines, protein levels of ANX4, ... al. p53 is a positive regulator of annexin IV FEBS Journal 278 (2011) 1470–1483 ª 2011 The Authors Journal compilation ª 2011 FEBS 1483 Wild-type p53 enhances...

Ngày tải lên: 22/03/2014, 16:20

... detect ODC or ADC enzy- matic activities in various strains of wild-type T. cruzi epimastigotes, by adding radioactive ornithine or arginine to intact parasites or cell extracts, gave negli- gible ... con- taining DNA spots (5 lg each) corresponding to inter- nal fragments of ADC or cruzipain genes. The latter (used as a control) is a housekeeping gene that encodes the main cysteine p...

Ngày tải lên: 19/02/2014, 07:20

... for bind- ing to ligand as well as for transactivation of reporter genes in cells. An EcR gene switch that includes a fusion protein containing VP16 activation domain, GAL4 DNA binding domain and ... DNA binding domain and VP16 activation domain. Various combinations of the EcR ligand binding domain, VP16 activation domain and GAL4 DNA binding domain were constructed (Fig. 1A) and tested...

Ngày tải lên: 16/03/2014, 12:20

... the interpretation is confirmed that the driving force for an optimal packing of the acyl chains is their linkage and distribution to the two glucosamines, causing the observed high inclination independent ... antagonistic activity, respectively. In other parameters such as acyl chain melting behaviour, antibody binding, activity in the Limulus lysate assay, and partially the binding of 3-...

Ngày tải lên: 23/03/2014, 13:20

... for protein folding in eukaryotic cells. Defects in protein folding contribute to a growing list of diseases, and advances in our understanding of the molec- ular details of protein folding are helping ... and understanding their specific func- tions and interrelationships is of considerable impor- tance, given their value to industry and medicine. Protein folding in vitro and in viv...

Ngày tải lên: 29/03/2014, 22:21

... DNA repair factors in situ in living cells has been providing us with striking information. For instance, even following exposure to ionizing radiation (IR), the DNA end-binding factor Ku moves ... proteins, including nucleolin, Ku80, nucleolinD, HMGB1 and HMGB2, were determined with an Applied Biosystems 492 model sequencer according to the instructions. For proteins that might be modifie...

Ngày tải lên: 19/02/2014, 06:20

... extracellular domain of b 2 integrin [5]. Annexin A5 was found to bind to the intracellular part of the b 5 integrin receptor Keywords annexins; calcium; heparin; protein– glycosaminoglycan interactions; ... cal- cium-binding site in domain III of this protein. Heparin binding Results from the heparin-binding assay show that ann- exin B1 possesses lectin properties. The protein binds to g...

Ngày tải lên: 07/03/2014, 12:20

... 4223 Stimulation of IRF-1 protein expression by IFN-c Generally, extra-cellular stimuli activate intracellular sign- aling cascades by stimulating the factors involved in the signaling cascades. Recently, ... z-IETD-fmk (Fig. 1 B), indicating that observed cell death is apoptotic cell death rather than necrotic cell death. Examination of cell m orphology also supported enhancer ac...

Ngày tải lên: 23/03/2014, 13:20

... has been shown to be involved in the fibrillogenesis of a-synuclein, a ‘natively unfolded’ protein [25–27] known to be involved in the pathogenesis of several neurodegenerative disorders [28]. ... (1999) Intrinsically unstruc- tured proteins: Re-assessing the protein structure-func- tion paradigm. J Mol Biol 293, 321–331. Proteolysis of proteins enhances fibrillogenesis P. Polverino de .....

Ngày tải lên: 30/03/2014, 16:20

... and the incidence of crisis recognition in hospitals. Their review of over 2000 events revealed an increase in events at certain times of the day, notably near nursing handoffs and physician rounding. ... emergency teams: deciphering clues to crises in hospitals Michael DeVita Associate Professor, Critical Care Medicine and Internal Medicine, University of Pittsburgh School of Medicine,...

Ngày tải lên: 25/10/2012, 10:45