... Hence, the dependence of k2 of wild-type and all mutants on the concentration of MgCl2was investigated at 278, 283, 288, 293 and 298 K.Table 1. Kinetic and thermodynamic parameters of the reaction ... investigated the dependence of the second-order rate constant of the overall reaction, k2, on both NaCl and MgCl2concentration.Information about the thermodynamic parameters of the transition ... reaction steps, not for the interaction of macromolecules in solution. Furthermore, the magnitude(but not the sign) of the thermodynamic parameters of activation measured with the same experimental...