Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx

... Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves Isabel Gavidia 1,2 , Pedro Pe ´ rez-Bermu ´ dez 2 and H. Ulrich Seitz 1 1 Center of Plant Molecular ... Biology (ZMBP), University of Tu ¨ bingen, Germany; 2 Department of Plant Biology, University of Valencia, Spain The aldo-keto reductase (AKR) superfamily comprises pr...

Ngày tải lên: 18/03/2014, 01:20

... Della Fazia 4 and Giuseppe Servillo 4 1 Department of Internal Medicine, University of Perugia, Italy; 2 Department of Pharmacological Sciences, University of Milan, Italy; 3 Department of Biochemical ... Universita Á ÔLa SapienzaÕ Roma, Italy; 4 Department of Biochemical Sciences and Molecular Biotechnology, University of Perugia, Italy Biosynthesis of cholesterol represe...

Ngày tải lên: 08/03/2014, 16:20

... vivo expression of IL-11 A range of tissues (head kidney, gills, liver, spleen, muscle, skin, intestine and brain) were collected from three healthy fish for analysis of constitutive expression of ... described previ- ously [48]. Brie y, 20 lg of total RNA per lane was trans- ferred from a 1.1% formaldehyde–Mops agarose gel to nylon membranes by capillary action and hybri...

Ngày tải lên: 07/03/2014, 16:20

... acceptance and enzymatic activity of those enzymes, if known, is highly diverse. With the increasing number o f s pecified plant enzymes of this g roup of the a/b- hydrolase f amily, i t might ... and degrades it immediately. Intra- cellular s eparation of MeJA synthesis and hydrolysis would be one way to avoid a treadmill situation, yet analysis of tomato MJE and Arabidopsis...

Ngày tải lên: 16/03/2014, 18:20

... contraction by activating ryanodine receptors of the sarcoplasmic Fig. 1. Isolation of tigrin from the Duvernoy’s glands of R. tigrinus tigrinus. (A) The extract from Duvernoy’s glands of R. tigrinus ... microliters of 4-vinylpyridine were then added, and alkylation was allowed to proceed for 3 h at room temperature. The S-pyridylethylated proteins were separated from the re...

Ngày tải lên: 18/03/2014, 01:20

... MgCl 2 without addition of any exogenous Ca 2+ . Phospho-amino acid analysis The phosphorylated 26-kDa band of PsCBL and the 58-kDa band of PsCIPK were separately eluted from the gel and hydrolysed in 6 n ... shown). Localization by immunofluorescence labelling and confocal microscopy Localization of PsCIPK and PsCBL was analysed by immunofluorescence labelling of tobacco BY...

Ngày tải lên: 19/02/2014, 07:20

... potential assemblies of HSF1 trimer, namely, two homotrimers (a 3 and b 3 ) and two heterotrimers (a 2 b 1 and a 1 b 2 ). The existence of the four types of trimer may be reflected in the broad band migrating ... 10.0; GAPEXT ,0.05; GAPDIST ,8; MAXDIV , 40; ENDGAPS ,off; NOPGAPS ,off; NOHGAPS ,off.Graphical output of the bootstrap figure was produced by the program TREEVIEW ....

Ngày tải lên: 19/02/2014, 12:20

... by HPLC and some of them were totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ( Fig. 2). Computer database searches based on the amino-acid sequences of ... reductase system in yeast and animals, w hile 1Cys-Prx may be r educed by a small thiol molecule such as glutathione. R ecently, 1Cys-Prx has been identi®ed i n y east and Arabi dopsis...

Ngày tải lên: 08/03/2014, 16:20

... height of the orbit and extends just above the top of the most-anterior ethmoid cells. By the age of 8 years, the top of the frontal sinuses is at the level of the orbital roof, and by the age of ... the age of 6 years. Their develop- ment is quite variable but effectively appears to start only after the second year of life [4,8,9]. By the age of 4 years, the averag...

Ngày tải lên: 25/10/2012, 11:04

... superiority of a combined assessment of the systolic and early diastolic mitral annular velocities by tissue Doppler imaging for the differenti- ation of restrictive cardiomyopathy from constrictive ... of the peak systolic longitudinal strain between segments with LE ≥ 51% and LE 1-50% 15 . By using the previously proposed cut-off value 15 for strain of –6.5 % a sens...

Ngày tải lên: 25/10/2012, 11:15