Báo cáo khoa học: Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways doc

... factor (Eur. J. Biochem. 269) 649 Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways Mohini Lutchman 1 , Anthony C. ... both association and dissociation phases of the interaction. Dematin and Ras-GRF2 associate in mouse brain lysate and in transfected epithelial cells T...

Ngày tải lên: 17/03/2014, 11:20

... evolution for these two proteins. To date, several Evi1-binding proteins have been identified using a candidate protein approach. It remains possible that Evi1 interacts with as yet unknown cellular proteins ... Rp-interacting proteins in yeast. The number of colonies obtained (clone A1 to A37), their growth on various selective media (SM), and their production of a- and b-galactos...

Ngày tải lên: 16/03/2014, 11:20

... contrast with MAP kinase, Rho mediates growth factor- dependent activation of c-fos via the serum response factor (SRF) [30]. Protein kinase C-related kinase 1 (PRK1), also termed protein kinase ... scraped from the upper side of the membrane and the membrane was subsequently placed into Giemsa stain for 60 min The membrane was mounted on a slide and the cells...

Ngày tải lên: 17/03/2014, 09:20

... [12–15]. SP-A and SP-D are lung surfactant proteins. Like MBL and the ficolins they are pattern-recognition molecules but do not share the property of binding the MASPs and therefore do not activate the ... N-acetylated and neutral carbohydrates [8] . It is synthesized in the liver and, like the other ficolins, has the potential of initiating the MBL ⁄ lectin complemen...

Ngày tải lên: 06/03/2014, 01:23

... (Zeiss). RESULTS NBR1 interacts with the PKC zeta interacting protein FEZ1 and the calcium and integrin binding protein CIB In order to identify proteins that interact with NBR1, a yeast two-hybrid ... NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expr...

Ngày tải lên: 08/03/2014, 10:20

... lanes 2 and 3, and the sequence of ARS and DARS-4 in Fig. 3A). The 20 nucleotide long region of the 5¢ end of the ARS (DARS-L; Fig. 3B: lane 4) and the A and U rich region located at the middle ... dimerization between PABP and IMP1 prior to binding the RNA could alter their binding site on the ARS. To understand the molecular nature of the interac- tion of I...

Ngày tải lên: 23/03/2014, 10:20

... to that caused by the depletion of core exo- some subunits [31]. Nop53p interacts with the nucleo- lar proteins Nop17p and Nip7p [31], both of which interact with the exosome and are involved ... determine whether Nop53p interacts with the pre-rRNA early during transcription, chromatin immunoprecipitation (ChIP) experiments were performed, using the fusion protein pr...

Ngày tải lên: 30/03/2014, 04:20

... of proteins containing both SHIP2 and filamin (data not shown) and the association of SHIP2 and p130 CAS has been reported by others [15]. A recent study of ArgBP2, another adaptor protein of the ... associated with the cell–matrix adhe- sion sites in a network of protein- like integrins and F-actin [35]. Our hypothesis is that the interaction of SHIP2 with Vinexin (and...

Ngày tải lên: 07/03/2014, 12:20

... NF-jB forms a complex with the IjBs and is thereby locked into the cytoplasm. IjBa is the best-characterized IjB, and is also a target of NF-jB itself. Thus, we focused on IjBa for further investigation. ... blocking, the cells were incubated with 3% bovine serum albumin in NaCl/P i for 1 h. Then, they were incubated with the anti-HA (3F10) or NF-jB-p65 antibody (F-6) over...

Ngày tải lên: 16/03/2014, 04:20

... L 11 is the label of the sibling of the parent node, etc. We also include: the lexical head of the current constituent (CL); the c-commanding lexical head (CC) and its POS (CCP); and the look-ahead ... using the perceptron algorithm. We follow that paper in fixing the weight of the generative model, rather than learning the weight along the the weights of the othe...

Ngày tải lên: 23/03/2014, 19:20