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Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot

Tài liệu Báo cáo khoa học: Cytokinin oxidase/dehydrogenase genes in barley and wheat docx

Tài liệu Báo cáo khoa học: Cytokinin oxidase/dehydrogenase genes in barley and wheat docx

... barleyregulate the active cyto kinin level principally in developinggrains, and that the biologically active cytokinins areprobably deactivated by other m etabolic pathways, suchas glycosylation, in the ... owing to their very low content in plant tissues. The ground-breaking cloning of the cytokinin oxidase maizegene ZmCKX 1 [9,10] o pened up the possibility for moredetailed study of cytokinin ... basiccharacterization of the CKX gene family in the cerealspecies Hordeum and Triticum, as well as report on the cloning of the fi rst two CKX genes of barley and demonstrate functionality for one of them in transgenictobacco...
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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... pro-moter use of the KLK11 gene appears to link to the splicing pathway of KLK11 mRNAs and to regulate the trafficking of KLK11 isoforms in the trans-Golginetwork. Such complex regulation of KLK11 ... expressing both isoforms of KLK11, and used human a neuroblastoma cell line,KP-N-YN, because of the transfection efficiency and expression of both KLK11 isoform 1 and isoform 2 in this cell line. ... the regulation and characteristics of these isoforms are unknown. We identified the first three exons (1a, 1b, and 1c) by determining their transcription initiation sites. Exon 1b con-tained the...
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Tài liệu Báo cáo khoa học: Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity docx

Tài liệu Báo cáo khoa học: Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity docx

... Miers, W.R. & Barrett, E.J. (1998) The role of insulin and otherhormones in the regulation of amino acid and protein metabolism in humans. J. Basic Clin. Physiol. Pharmacol. 9, 235–253.3. ... nature of the insulin- (namely the IR) and arsenite-induced tyrosine kinases, and fur-thermore they suggest that tyrosine kinase activity is arequirement for arsenite-mediated induction of glucoseuptake ... whetherthis tyrosine kinase activity is required for arsenite-inducedglucose uptake, we applied the tyrosine kinase inhibitor,tyrphostin A25. Insulin-induced IRS and caveolin-1 tyro-sine...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "NATURAL VS. PRECISE CONCISE LANGUAGES FOR HUMAN OPERATION OF COMPUTERS: RESEARCH ISSUES AND EXPERIMENTAL APPROACHES" doc

... opers of interactive systems and in evaluating the impor- tance of the user's familiarity with: i) the problem domain 2) the data in the computer 3) the available commands 4) typing skills ... been handled, than the operator's con- viction that the terminal and thus ~he @~t.e~ ~re in control, have 'a mind of their own,' or are tugging against rather than observing the ... are in control of the system and not the system in control of them. Equally, nothing can be more damaging to satisfactory system opemtion, regardless of how well all other aspects of the imple-...
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Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx

Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx

... annealed and inserted into the BglII and HindIIIsites of pEGFP-NLS. The oligonucleotide fragment forNES-NLS was flanked with BglII and BamHI, and theninserted into the BamHI site of pGEX-hGFP. ... Microinjection of NLS-containing substrates in a myoblast. The movie represents the NLS microin-jection experiment shown in Fig. 6A.Video S2. Microinjection of NLS-containing substrates in a myotube. The ... component of the NPC [18] and was increased within individualNPCs during muscle differentiation. Thus, we hypothe-sized that increased Nup358 in individual NPCs wasresponsible for the change in NPC...
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Báo cáo khoa học: Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding pot

Báo cáo khoa học: Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding pot

... because the substitution of cysteinewith serine results in a significant lowering of the thermodynamic and kinetic stability parameters of SsMTAPII and PfPNP [10,12]. The stabilizing role of the ... cysteines with serines significantlyaffects both their thermodynamic and kinetic stability[10,12], suggesting the involvement of the cysteine pair in the thermal stabilization of both enzymes. In ... monitoring the refolding of SsMTAPII, PfPNP and their mutants lacking the C-terminalcysteine pair after guanidine-induced unfolding, we demonstrated that the CXC motif is required for the folding of these...
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Báo cáo khoa học: Peroxiredoxins as cellular guardians in Sulfolobus solfataricus – characterization of Bcp1, Bcp3 and Bcp4 pot

Báo cáo khoa học: Peroxiredoxins as cellular guardians in Sulfolobus solfataricus – characterization of Bcp1, Bcp3 and Bcp4 pot

... tested (Fig. 7C). At this point, we deter-mined the thermoresistance levels of the enzymes byincubating them for varying periods at 80, 90, 95 and 100 °C and then assaying their respective residual ... atypical, depending on the location of Cys-SRresidue. In typical 2-Cys-Prxs, the Cys-SPOH reactswith the Cys-SRresidue located in the C-terminalsequence of the other subunit of the antiparallelhomodimer. ... Da and a theoretical pI of 7.65;bcp3 encodes a putative protein of 149 amino acidswith a predicted molecular mass of 16 976.42 Da and a theoretical pI of 8.84. Comparison of the sequencesof...
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Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot

Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot

... processing of the APP family of proteins. BACE1 expression regulated the levels and species of full-lengthAPLP1, APP and APLP2, of their shed ectodomains, and of their mem-brane-bound C-terminal ... loss of BACE1activity. These results indicate that BACE1 is involved in regulating ecto-domain shedding, maturation and trafficking of the APP family of pro-teins. Consequently, whereas inhibition ... family of proteins isbelieved to play important roles in both the peripheral and central nervous systems [6]. In the former, theyare involved in the formation and correct functioning of the neuromuscular...
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Báo cáo khóa học: Mycoplasma pneumoniae HPr kinase/phosphorylase Assigning functional roles to the P-loop and the HPr kinase/phosphorylase signature sequence motif doc

Báo cáo khóa học: Mycoplasma pneumoniae HPr kinase/phosphorylase Assigning functional roles to the P-loop and the HPr kinase/phosphorylase signature sequence motif doc

... S161 is involved in the catalytic activity, in addition to its role in triphosphate binding. In the HPrK/P of B. subtilis, the corresponding S160A mutationresulted in loss of ATP binding and enzymatic ... unknown. The three amino acidsG159, K160, and S161 are involved in binding of the triphosphate moiety of ATP or GTP [19,32]. The muta-tions G159A and K160A completely abolish the binding of ATP ... trimers. The monomers are composed of twodomains. The N-terminal domains are exposed to the environment, whereas the C-terminal domains form the compact core of the protein. The C-terminal domainsalone...
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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc

... FEBSbean and soybean. When binding of arginine in the presence of tRNA was investigated, some changes in the binding architecture were observed [35], in thatN(153:Y), in addition to interacting with ... level attained in the pres-ence of L-arginine was compared to that in the presence of 1 mM of the analogue indicated, using [32P]-labelled jack bean transcript tRNA.Inset: Activity of arginyl-tRNA ... arginylated in the presence of arginyl-tRNA synthetase fromjack bean (diagonal shading) or soybean (vertical shading), and the level of charging was compared with that in the presence of the E....
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