Báo cáo khoa học: The mystery of nonclassical protein secretion A current view on cargo proteins and potential export routes pdf

... biochemical analysis of the export apparatus. It is also a major problem that, in most cases, only very limited information is available about the motifs that target cargo proteins to the nonclassical ... proteins in mammalian cells revealed the existence of a machinery that is capable of translocating the protein across the plasma membrane [103], demonstrati...

Ngày tải lên: 17/03/2014, 03:20

... interaction. (A) The sequential binding of the ligands to each half of the receptor dimer induces the recruitment of two molecules of b-arrestin and then the activation of ERK. (B) Only one molecule of ... plasma membrane and to stimulate ERK1 ⁄ 2 phosphorylation. All these data indicate quite clearly that homo-oligomerization and hetero-oligomerization have a pi...

Ngày tải lên: 16/03/2014, 22:20

... addition of information to the model. This interaction of the evaluation of a formula and the expansion of the model produces several linguistically interesting side-effects, and these have ... evaluates a formula with respect to a model has been augmented with a table that contains each ~-expression and the ima6e of its denotation in the current...

Ngày tải lên: 21/02/2014, 20:20

... including anaesthetic and surgical procedures, as well as animal man- agement, have been reviewed and approved, and were performed in accordance with the relevant China national legislations and regulations. Recombinant ... blot analysis showed that the effi- ciency of PrP Sc propagation was enhanced by the addition of NADPH, which was closely related to increasing NADPH conc...

Ngày tải lên: 07/03/2014, 03:20

... binding at the active site of RNase A. The r.m.s.d. between the RNase–AMP complex and the unliganded protein are 0.43, 0.44 and 0.59 A ˚ for the Ca, main chain and side chain atoms of 123 equivalent ... R free value. The second AMP conformation has the phosphoryl group away from the P 1 subsite and as it is a minor conformation it was not included in the s...

Ngày tải lên: 07/03/2014, 21:20

... primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTC ACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢). For PCR, the reaction mix consisted of 1 · Taq buffer contain- ing 1.5 ... the tiger prawn Penaeus monodon. As there is a high degree of sequence similarity and structural conservation of these genes and gene products, confirmation of the true identi...

Ngày tải lên: 16/03/2014, 05:20

... probe the functional effects of dimeriza- tion ⁄ oligomerization on mechanisms of ligand binding and extent of activation (i.e. pharmacological efficacy), as well as on the involvement of protein protein ... information can be used to predict pro- bable regions involved in protein protein interactions. Many of the limitations and considerations of accuracy and r...

Ngày tải lên: 16/03/2014, 22:20

... Plecoptera, Dermaptera, Orthop- tera, Phasmatodea, Mantodea, Isoptera and Blattaria, but not in Protura, Diplura, Ephemeroptera, Odonata and the Eumetabola (Holometabola + Hemiptera) (Fig. 3). In addition, ... Hexapoda and provides strong evidence for the monophyly of the Polyneoptera (Plecoptera, Dermaptera, Orthop- tera, Phasmatodea, Mantodea, Isoptera, Blattaria) and the...

Ngày tải lên: 18/02/2014, 08:20

... (DTT), aggregation and precipitation of a- lactalbumin commenced after 25 min and reached a plateau after 90 min. The amount of DTT-induced aggregation of a- lactalbumin was increased in a concentration-dependent ... ordered and disordered protein aggregation Heath Ecroyd and John A. Carver School of Chemistry and Physics, The University of Adelaide, Australia...

Ngày tải lên: 18/02/2014, 16:20

... digestion of the DNA to mononucleosome size of samples con- sisting of a mixture of hepta-, octa- and nonanucleosomes and half the amount of hexa- and decanucleosomes (6- to 10-mer sample). (A) (a) Nonamer ... mononucleosomes and the band S. DNA gel electrophoresis of the fractions from Fig. 3D,E showed that approximately 90% of the DNA in the main peak as...

Ngày tải lên: 18/02/2014, 18:20