Báo cáo khoa học: Saccharomyces cerevisiae Pip2p–Oaf1p regulates PEX25 transcription through an adenine-less ORE ppt

... 2003 Saccharomyces cerevisiae Pip2p–Oaf1p regulates PEX25 transcription through an adenine-less ORE Hanspeter Rottensteiner 1 , Andreas Hartig 2 , Barbara Hamilton 2 , Helmut Ruis 2 , Ralf Erdmann 1 and ... PEX25 ORE (lane 6) was com- pared with the OREs of POT1/FOX3 (lane 2), POX1/FOX1 (lane 4), and ANT1 (lane 5). (B) Labeled PEX25 ORE was assayed as above. In the...

Ngày tải lên: 17/03/2014, 03:20

... mutants aW303DCOQ2 (coq2) and aW303DCOQ10 (coq10); the bc1-deficient mutant aW303DBCS1 (bcs1); and wild-type cells and coq10 mutants transformed with pYES2–AfAOX (wt + AOX and coq10 + AOX) and ... H 2 O 2 release in wild-type and AOX transformants, but had no effect in the coq2 and bcs1 mutants over and above the rate seen without the inhibitor (Fig. 2B). The ability of antimycin A to stim- ul...

Ngày tải lên: 06/03/2014, 22:21

... glycosylphosphatidylinositol; GPI-MT, GPI a-mannosyltransferase; JbaM, jack bean a-mannosidase; Man 1 -GPI, mannosyl GPI; Man 2 -GPI, dimannosyl GPI; Man 3 -GPI, trimannosyl GPI; Man 4 -GPI, tetramannosyl GPI; PI-PLC, phospholipase ... example, a fourth mannose (Man4) is side-branched to the third core mannose (Man3) of all yeast GPIs [9] and of certain human GPIs [10–12], and additional side...

Ngày tải lên: 07/03/2014, 16:20

... (1998) Identification of the MNN2 and MNN5 mannosyltransferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae. J Biol Chem 273, 26836–26843. 11 ... initiated by a1,6-mannosyltransf- erase (Och1p), which transfers an a1,6-mannose to the a1,3-linked mannose that is attached to the b1,4-man- nose of the Man 8 GlcNAc 2 [5,6]. Th...

Ngày tải lên: 23/03/2014, 10:20

... either directly analysed (lanes 1–6) or immunoprecipitated (IP) using antiserum against YidC (lanes 7 and 9) or SecY (lanes 8 and 10). (C) The YidC ⁄ EcCox18 content of the IMVs used was analysed by ... [6]. Acknowledgements We thank Corinne M. ten Hagen-Jongman for techni- cal assistance and Wouter Jong, Dirk-Jan Scheffers and E. van Bloois et al. Evolutionarily conserved function of S. cer...

Ngày tải lên: 30/03/2014, 03:20

... protein regulates alternative splicing of an aberrant pseudoexon in NF1 Michela Raponi 1 , Emanuele Buratti 2 , Miriam Llorian 3 , Cristiana Stuani 2 , Christopher W. J. Smith 3 and Diana Baralle 1 1 ... Hybond-C Extra membrane (Amersham, Chalfont St Giles, UK), and antibody recognition was then performed using several in-house antibodies against PTB, hnRNP A1 ⁄ A2 ⁄ C and H proteins and...

Ngày tải lên: 16/03/2014, 04:20

... find relevant words, not to provide the meaning of words. 3 Paradigme: A Field for Measuring Similarity We analyse word meaning in terms of the seman- tic space defined by a semantic network, ... of links: a rdf4rant and a rdfdrd. A r~f~rant of a node consists of several subrdfdrants correspond to the units of Giossdme. As shown in Figure 2 and 3, a morphological analysis maps the .....

Ngày tải lên: 24/03/2014, 05:21

... glyphosate accumulates in vacuoles of resis- tant plants at a markedly faster rate than in sensitive plants [61]. Analysis of the transcriptome of resistant and sensitive lines revealed upregulation ... globular domain colored pale green and the C-terminal domain colored brown. The helix containing Pro101 is colored magenta, and the S3P and glyphosate molecules are colored green and yellow, r...

Ngày tải lên: 14/02/2014, 14:20

... pervanadate-treated cells with an antibody against total tau revealed decreased electrophoretic mobility of tau, with the appearance of an  68-kDa tau species in wild-type and all of the mutant ... [8]. Recently, we and others have highlighted the impor- tance of tau interactions with the membrane-anchored nonreceptor tyrosine kinase Fyn during A b-mediated neurodegeneration in cell and...

Ngày tải lên: 14/02/2014, 14:20

... tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse [11,37]. Foxp3 is also highly expressed in lymphoid organs, ... & Nakanishi T (2008) Molecular cloning and expression analysis of T-bet in ginbuna crucian carp (Carassius auratus langsdorfii). Mol Immunol 45, 127–136. 28 Guo CJ, Zhang YF, Yang LS, Yang XB...

Ngày tải lên: 16/02/2014, 09:20