Báo cáo khoa học: Identification and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot

... understand- ing of the XA metabolism in the mosquito and offers a new possible target for the development of insecticides and ⁄ or transmission blocking agents. Results and Discussion Isolation of the ... account of their roles in the physiological tuning of the central nervous system and in the etiogenesis and progression of several human neurodegenerative...

Ngày tải lên: 16/03/2014, 23:20

... tissues in the upper or lower part of the barnacle in the same manner as described above. The upper part included the cirri, tho- rax, prosoma and hemolymph, and the lower part included the mantle, ... 0.00 a The amino acid composition calculated from the deduced sequence. b The amino acid composition analyzed by amino acid analysis. c Sum of the numbers of Asp...

Ngày tải lên: 16/03/2014, 05:20

... 1A) and the 3¢ primer described above. The 25 lL of PCR mixture consisted of 1 unit of DyNazyme EXT, 0.3 l M of each primer, 0.2 m M dNTP and 1.5 ng of plasmid DNA. Reactions were run using the following ... X17247) and rat ST3Gal III (clone ST3N-1 [31]), the 5¢ end of these genes were modified. The cytoplasmic tail and the trans- membrane domain were deleted a...

Ngày tải lên: 08/03/2014, 08:20

... organization to the human IL-18 gene, consisting of six exons and five introns. The Fig. 2. Comparison of the genomic organization of IL-18 and IL-1b genes in human, rainbow trout and the predicted ... similarity with the other members of the IL-1 family; 30–38% with IL-1a, 26–36% with IL-1b and  30% with IL-1Ra (data not shown). To further analyse the relationsh...

Ngày tải lên: 16/03/2014, 16:20

... from cleavage of the signal peptide and the propeptide, which happens in the maturation process for every conotoxin [8], the removal of the two additional resi- dues Leu and Val at the C-terminus is rather ... cDNA and the deduced precursor sequence of conomarphin. The signal pep- tide is shadowed and the mature peptide is underlined. The polyA signal AATAAA...

Ngày tải lên: 18/02/2014, 17:20

... mark the boundary of the predicted substrate-binding pocket, reveals that some of them are conserved in the inac- tive isoform (i.e. Trp191 and Phe294). Even though the precise role of these ... reduced the activity. It can be hypothesized that these salts might chelate the metal and efficiently remove it from the protein at acidic pH. Both the different sensitivity t...

Ngày tải lên: 19/02/2014, 02:20

... sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numbers on the right of the figure indicate the positions of the nucleotide sequence. The ... allergens in the pollen. As a consequence, individuals allergic to BGP require individual diagnosis and therapy, and an understand- ing of the structure of...

Ngày tải lên: 07/03/2014, 12:20

... further study. The concentration of the proteins was determined by the method of Lowry et al. [14] and the purity was examined by SDS/PAGE [15]. Assay of human PKIb activity The activity of purified ... cm )1 bands canbeassignedtoa-helix in the human PKIb; the 1666 and 1247 cm )1 bands are the characteristic frequencies of the random coil structure. Meanwhile,...

Ngày tải lên: 07/03/2014, 15:20

... Purification and biochemical characterization of some of the properties of recombinant human kynureninase Harold A. Walsh and Nigel P. Botting School of Chemistry, University of St Andrews, St Andrews, ... enzyme. When the lines intersect above the x-axis then K i < K i ¢, and when the lines intersect below the x-axis then K i > K 0 i (in both instances the...

Ngày tải lên: 08/03/2014, 22:20

... for the promiscuous nature of the synthesis functions performed by the prim–pol domain and to discriminate between the functions of in vitro primase and polymerase. Another finding of our biochemical ... 2008 The Authors Journal compilation ª 2008 FEBS 4391 Zn-binding motif and the two disulfide bonds respec- tively connecting the a4-helix to the b4 strand and t...

Ngày tải lên: 18/02/2014, 18:20