Báo cáo khoa học: Identiﬁcation and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot

... understand-ing of the XA metabolism in the mosquito and offers anew possible target for the development of insecticides and ⁄ or transmission blocking agents.Results and DiscussionIsolation of the ... account of their roles in the physiological tuning of the central nervous system and in the etiogenesis and progression of several humanneurodegenerative diseases (reviewed in [1–4]). One of the ... 5653–5662 ª 2005 FEBS Identiﬁcation and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase Franca Rossi1, Fabrizio Lombardo2, Alessandra Paglino1, Camilla...

Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

... tissues in the upper or lower part of the barnacle in the same manneras described above. The upper part included the cirri, tho-rax, prosoma and hemolymph, and the lower part included the mantle, ... 0.00a The amino acid composition calculated from the deduced sequence.b The amino acid composition analyzed by amino acid analysis.cSum of the numbers of Asp and Asn.dSum of the numbers of ... polycrystalline gold and alkylated gold. The arrows and thick arrows indicate the starts of sample loading(2 lM) and washing by the running buffer, respectively. The processes of sample loading and washing...

Báo cáo khoa học: Identiﬁcation and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

... 1A) and the 3¢ primer described above. The 25 lL of PCR mixture consisted of 1 unit of DyNazyme EXT, 0.3 lM of each primer, 0.2 mMdNTP and 1.5 ng of plasmid DNA. Reactions were run using the following ... X17247) and rat ST3Gal III (clone ST3N-1 [31]), the 5¢ end of thesegenes were modiﬁed. The cytoplasmic tail and the trans-membrane domain were deleted and the signal peptidesequence of a viral ... proﬁle (B) of human ST6Gal II, and (C) comparison of the sia-lylmotifs L, S and VS of hST6Gal II with those of previously clonedsialyltransferases. (A) Numbering of the cDNA begins with the initi-ation...

Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... organization to the humanIL-18 gene, consisting of six exons and ﬁve introns. The Fig. 2. Comparison of the genomic organization of IL-18 and IL-1b genes in human, rainbow trout and the predicted ... similarity with the other members of the IL-1family; 30–38% with IL-1a, 26–36% with IL-1b and  30%with IL-1Ra (data not shown). To further analyse the relationship of trout IL-18 with the other three ... poly(I:C), rIL-1b or a mixture of LPS and rIL-1b as described in the materials and methods. In C, two of the three ﬁsh investigated are shown and the average ratio of the IL-18 expression relative...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... fromcleavage of the signal peptide and the propeptide,which happens in the maturation process for everyconotoxin [8], the removal of the two additional resi-dues Leu and Val at the C-terminus is rather ... cDNA and the deduced precursorsequence of conomarphin. The signal pep-tide is shadowed and the mature peptide isunderlined. The polyA signal AATAAA in the 3¢-UTR is also underlined. The cDNA of ... with the presence of ashort 310-helix. A random coil rather than a compactloop in the region of residues 1–10 existed inl-Phe13-conomarphin.Fig. 3. The overlay of the backbone atomsfor the...

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... mark the boundary of the predicted substrate-binding pocket,reveals that some of them are conserved in the inac-tive isoform (i.e. Trp191 and Phe294). Even though the precise role of these ... reduced the activity. Itcan be hypothesized that these salts might chelate the metal and efﬁciently remove it from the protein atacidic pH.Both the different sensitivity to EDTA, and the lower ... ACMSD. The presence of the metal-locofactor in human ACMSD might also be inferredby our study on the enzyme pH dependence, showingthat the increase of the concentrations of succinate and 4-morpholineethanesulfonic...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numberson the right of the ﬁgure indicate the positions of the nucleotide sequence. The ... allergens in the pollen.As a consequence, individuals allergic to BGP requireindividual diagnosis and therapy, and an understand-ing of the structure of these allergens is essential to the improvement ... to the N-terminal portion of the allergen.Based on this partial sequence, two speciﬁc primerswere designed and used together with the anchor pri-mer AP2 to obtain the 5¢ and 3¢ portions of the Cyn...

Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... further study. The concentration of the proteins was determined by the method of Lowry et al. [14] and the purity was examinedby SDS/PAGE [15].Assay of human PKIb activity The activity of puriﬁed ... cm)1bandscanbeassignedtoa-helix in the human PKIb; the 1666 and 1247 cm)1bands are the characteristic frequencies of the random coil structure. Meanwhile, the 1682 and 1255 cm)1bands can be assigned to ... [21]. The quantitative contributions of the individual amide I¢component bands, determined by band ﬁtting of the absorbance spectrum of Fig. 4A, are shown in Fig. 6 and Table 2. From Table 2, the...

Báo cáo Y học: Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninase pptx

... Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninaseHarold A. Walsh and Nigel P. BottingSchool of Chemistry, University of St Andrews, St Andrews, ... enzyme.When the lines intersect above the x-axis then Ki< Ki¢, and when the lines intersect below the x-axis then Ki> K0i(in both instances the lines have to intersect to the left of the y-axis). ... of humankynureninase in an appropriate host that would yieldsufﬁcient quantities of protein to permit identiﬁcation and biochemical characterization of the enzyme and investiga-tion into the...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... for the promiscuous nature of the synthesis functions performed by the prim–poldomain and to discriminate between the functions of in vitro primase and polymerase.Another ﬁnding of our biochemical ... 2008 The Authors Journal compilation ª 2008 FEBS 4391Zn-binding motif and the two disulﬁde bonds respec-tively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the ... analyses of the predictedamino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro-teins. The...

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