Báo cáo khoa học: Cdk2 activity is dispensable for triggerin

Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

... are induced in BJAB cells following the initiation of apoptosis, but KIPase activity remains constant. In order to ascertain whether KIPase behaves in a proliferation dependent manner in cells ... described for the human B-lymphoma cell line BJAB [1]. In this case, inhibition of KIPase activity using a cell-permeable inhibitor resulted in an increase in p27 KIP1 abun...

Ngày tải lên : 19/02/2014, 13:20

8
442
0

Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx

... that cells expressing wild-type HFE displayed a striking decrease in cell-surface binding of transferrin, and that little or no reduction in transferrin binding was observed in cells expressing ... other genes involved in iron metabolism. This includes hepci- din, a 25 amino acid antimicrobial peptide that is expressed in liver cells and is now recognized as a key regula...

Ngày tải lên : 06/03/2014, 22:21

16
538
0

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... be used as a substrate for testing AUH activity in a routine setting. Indeed, we were able to show that AUH activity in ﬁbro- blasts can be determined by monitoring the formation of 3-hydroxyglutaryl-CoA. ... hydratase activity is associated with mutations in the AUH gene. This indi- cated that AUH, in addition to its RNA binding func- tion, must play an important role in...

Ngày tải lên : 19/02/2014, 07:20

11
625
0

Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

... This suggests that the chelators may also be acting intracellularly, inhibiting Fe incorporation into ferritin. In comparison, the proportion of 59 Fe in ferritin did not change signiﬁcantly in ... cells and used in Fe-dependent reactions essential for growth and proliferation, is uncertain. Hence, it is of interest to investigate the uptake and metabolism of NTBI in normal...

Ngày tải lên : 20/02/2014, 11:20

10
545
0

Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a ﬂuorescence resonance energy transfer–ﬂuorescence lifetime imaging microscopy technique docx

... ubiquitin ligases. The ubiquitin-like domain of elongin B was found to be necessary for binding to elongin C [9]. pVHL shares a common binding site with elongin A on elongin C, and no direct interaction occurs ... encoding: (C) cerulean-elongin B and elongin C- citrine; (D) elongin B-cerulean and elongin C-citrine; (E) cerulean- elongin B and citrine-elongin C; and (F) elongin B-cerulean an...

Ngày tải lên : 16/03/2014, 05:20

9
420
0

Báo cáo Y học: Effects of ATP depletion and phosphate analogues on P-glycoprotein conformation in live cells docx

... depleting agents, all the phosphate analogues increased daunorubicin accumulation in Pgp- expressing cells. Prelabeling of ATP depleted cells with UIC2 completely abolished the subsequent binding ... MM12.10, in accordance with the enhanced binding of the first mAb. Vanadate and beryllium fluoride, but not fluoro-aluminate, reversed the effect of cyclosporin A, preventing UIC2 binding and...

Ngày tải lên : 08/03/2014, 23:20

6
590
0

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... examined calpain 7–IST1 interactions by in vitro and in vivo binding experiments, using puriﬁed recombinant proteins and cultured mammalian cells expressing epitope-tagged proteins. We also investigated ... be involved in virus budding. The con- servation of the YPX(n)L motif for binding to ALIX ⁄ PalA, however, hints an approaching way to search for potential calpain 7 substrat...

Ngày tải lên : 18/02/2014, 04:20

15
505
0

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... double- ringed BchI-BchD structure the ATPase activity of BchI is blocked. A conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin-binding ... coordination motif characteristic for integrin I domains [10]. Integrins are known to participate in cell–matrix and cell–cell interactions [14,15]. They are involved in signalli...

Ngày tải lên : 19/02/2014, 12:20

7
475
0

Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modiﬁcations and activity-based analyses doc

... FEBS analysis of purine-binding proteins in a total lysate of human Jurkat T -cells [40]. COFRADIC analysis of protein processing ) protease degradomics Protein processing introduces novel protein fragments that ... character- ize ATP-binding and GTP-binding proteins in cells, we proﬁled purine-binding proteins on a proteome scale [40]. For this purpose, we used 5¢- p-ﬂuoro- sulfonyl...

Ngày tải lên : 18/02/2014, 16:20

13
578
0

Tài liệu Báo cáo khoa học: Human Cdc45 is a proliferation-associated antigen pdf

... proliferating cells. More- over, Cdc45 is much less abundant than the minichromosome maintenance (Mcm) proteins in human cells, supporting the concept that origin binding of Cdc45 is rate limiting for ... macromolecular complex consisting of avidin and biotinylated peroxidase (Vector Laboratories, Burlingame, CA), which retains biotin-binding sites. The staining reaction was perform...

Ngày tải lên : 19/02/2014, 00:20

16
504
0

Báo cáo khoa học: Cdk2 activity is dispensable for triggering replicon initiation after transient hypoxia in T24 cells docx

Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx