Báo cáo khoa học: Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans docx

... domains or putative GPI unit, if one exists in CLAP as it does in Drosophila fasciclin I and many other fasciclin-containing proteins [36,46]. Fas I domains in proteins are a lmost always found in ... domains [37]. Of importance to understanding the structure and function of CLAP, is that most proteins containing Fas I domains are anchored to cell membranes through a GPI unit...

Ngày tải lên: 07/03/2014, 16:20

... oniae and E. coli infection in humans [49,50]. Binding of Cry1Ac to proteins of 68-kDa in size in ligand blots of H. virescens BBMV has been reported previously [1,5,11]. Our ligand blotting and ... glycoproteins that are part of the C binding site group and r esistance to C ry1Ac in the H. virescens YHD2 strain [11]. Cry1 toxin-binding proteins of 60- to 80-kDa in...

Ngày tải lên: 23/03/2014, 13:20

... pos- sess solanesyl and decaprenyl diphosphate synthases as the determining enzyme for a type of ubiquinone. Here we show that murine and human solanesyl and decaprenyl diphosphate synthases ... 5609 investigate mammalian long-chain trans-polyprenyl diphosphate synthases. Here we describe the identification and characteriza- tion of solanesyl and decaprenyl...

Ngày tải lên: 16/03/2014, 23:20

... range of pH from 3 to 9 (not shown). The effect of staurosporine, a potent pro- tein kinase inhibitor was also examined. Pre-incubation of inhibitor with StkP inhibited its kinase activity in a dose-dependent ... mutant strains is S1 ribosomal protein involved in RNA bind- ing. Phosphorylation of this protein on serine residue was described in E. coli [22] and C. glutamicum [1...

Ngày tải lên: 16/03/2014, 18:20

... reciprocal plots yielded lines converging to the left of the ordinate axis. Secondary plots of V )1 intercepts and of slopes were linear for either of the substrates. These results indicated that the ... earlier findings of d’Harlingue and Camara [15] and may be, indeed, due to the tendency of membrane proteins to aggregate. This aggregation phenomenon may also explain the in...

Ngày tải lên: 17/03/2014, 09:20

... AM, van Lint J & Burgering BM (1998) Essential role for protein kinase B (PKB) in insulin-induced glycogen synthase kinase 3 inactivation. Characterization of dominant-negative mutant of PKB. ... S6 kinase; PDK1, phosphoinositide-dependent protein kinase-1; PKA, protein kinase A; PKB, protein kinase B; PKC, protein kinase C; p-Ser, phospho-serine; PtdIns3K, phosphatidylinositol 3...

Ngày tải lên: 23/03/2014, 11:20

... 1343 where the relevant increase of m 2 produces the relevant intensity changes of ML ⁄ TG signals. In principle, such an increase can be due to both a decrease in c 1 and an increase in p 1 after growth ... sine function was applied in both dimensions of the time domain before Fourier transformation. Integration of 1D and 2D signals was performed using 1D winnmr and 2D...

Ngày tải lên: 18/02/2014, 13:20

... and storage of protein Optimal growth conditions were standardized in order to obtain the highest amount of total soluble protein, as assessed by the intensity of a 60 kDa band on SDS/ PAGE and ... Underlining indicates insertion typical of plant and trypanosomatid i-PGAMs. The amino acids involved in chelation of metal ions are indicated with a circle d: 1, corresponding...

Ngày tải lên: 16/03/2014, 16:20

... findings argue for an early evolutionary origin and a high con- servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPs in humans and insects ... transcripts during development and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and tem...

Ngày tải lên: 19/02/2014, 00:20

... indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of each RT-PCR ... results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step of protein synthesis and thereby in part det...

Ngày tải lên: 19/02/2014, 06:20