Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot

... equal protein loading, b-actin was used as an internal control. Cell invasion assay The migratory and invasive responses of Saos-2 cells were determined using the BD Biocoat Matrigel Invasion Cham- ber ... Downregulation of urokinase plasminogen acti- vator receptor expression inhibits Erk signalling with concomitant suppression of invasiveness due to loss of uPAR-beta...

Ngày tải lên: 16/03/2014, 22:20

... changes of C-LytA upon binding of these alkaloids are different to those induced by choline, suggesting a different mode of binding. In vitro inhibi- tion assays of three pneumococcal, choline-dependent ... 2006 FEBS Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines Beatriz Maestro 1 , Ana Gonza ´ lez 2 , Pedro Garcı ´a 2...

Ngày tải lên: 19/02/2014, 05:20

... (2001) Suppression of urokinase expression and invasiveness by urinary trypsin inhibitor is mediated through inhibition of protein kinase C- and MEK/ERK/c-Jun-dependent signaling pathways. J. Biol. ... with intact bikunin or bikunin derivatives is shown in Fig. 6 and Table 1. Exposure of cells to bikunin, bikunin-a and bikunin-n resulted in decrease in the amount...

Ngày tải lên: 17/03/2014, 11:20

... fusion of GAL4 DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vector LmRXR A fusion of VP16 activation domain and LmRXR ligand-binding ... chemical ligand, followed by rapid and robust induction of transgene expression in the presence of a low concen- tration (nanomolar) of a chemical ligand. In an effo...

Ngày tải lên: 15/03/2014, 23:20

... affects gene expression in 293 cells The affect of the AMK RG-121150 on gene expression in 293 cells was analyzed using microarray and qRT- PCR. In microarray analysis, a total of 636 genes were up-regulated ... for 3 days resulted in the death of 70% of the cells as indicated by observation of cell morphology. To determine the possible genes and path-...

Ngày tải lên: 30/03/2014, 03:20

... down-regulating vimentin in the absence and in the presence of MTX. Targeting of vimentin was carried out by lipofecting aODN-VIM into paren- tal HT29 cells causing a decrease of 60% in the expression of ... h in the dark. Afterwards, cells were centri- fuged at 800 g 5 min and resuspended in annexin-binding buffer containing propidium iodide (Sigma) at 1 lgÆmL )...

Ngày tải lên: 07/03/2014, 16:20

... environment and are increasingly making their way into the food chain. Considering that sulfation is widely used in vivo for the inactivation and/ or excretion of xenobiotic compounds, we became interested ... fetal bovine serum, 0.5% trout serum, 0.1 mgÆmL )1 bovine insulin, 50 lgÆmL )1 strepto- mycin sulfate, and 30 lgÆmL )1 penicillin G. Confluent zebrafish liver cells grown in...

Ngày tải lên: 08/03/2014, 02:20

... promoter fragment of the human Cyr61/CCN1 gene is functional and induces a reporter gene activity in S1P-treated cells. Using a combination of cis-element mutagenesis and expression of dominant negative inhibitors ... specific inhibitors of p38 and RhoA alter the phosphorylation state of both CREB and the CREB kinase, MSK1 in S1P-treated cells indicating that S1P...

Ngày tải lên: 08/03/2014, 08:20

... the number of TH-expressing cells in serial fields (Fig. 3). In contrast, in hypothalamic groups of cells, the overlap was only partial between recombination and TH expression. Whereas in the periventricular organ ... to inactivate the cAMP-response-element-binding (CREB) gene [32]. In combination with the D1Cre line, which allows targeting of recombination in dopami...

Ngày tải lên: 30/03/2014, 08:20

... or cofactor. In detail, the N-terminal module is the homocysteine (Hcy)-binding domain; the second domain binds 5-methyltetrahydrofolate, the third domain binds CH 3 -Cbl; and the fourth domain ... the interactions occurring at the binding site, and to develop a tool that could assist further optimization of inhibitors, a molecular model of the methyltetra- hydrofolate-binding domai...

Ngày tải lên: 19/02/2014, 05:20