Báo cáo khoa học: Adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases ppt

Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf

... quantifi-cation module in the software package. The PCR reactions were performed in a ﬁnal volume of 10 lL containing 1 lL of cDNA sample, 0.4 lm each primer, and 5 lL of the 2·High Resolution Melting ... openquestions. The use of mutant (e.g. deletion and extracopy numbers) and transgenic (RNAi and overexpres-sion) lines will help us to better understand therelationship between Hsps and cold tolerance.Experimental ... with$ 10 mL of standard food medium. Flies of the RA groupwere transferred to vials containing $ 10 mL of 1% agar,which provides a source of water but not nutrients. Fordetermination of any cumulative...

Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt

... site. A NOESY spectrum of 2 and 3 in the presence of a small quantity of proteaserevealed an intermolecular cross-peak between themethyl group of 3 and the phthalazine ring of 2,asexpected for ... the exception of prolines and a few resi-dues with very broad amide peaks. The resonances of the peptide connecting NS2B and NS3pro appeared atchemical shifts characteristic of random coil confor-mation ... site of theprotease.In the ﬁrst experiment, we compared the15N-HSQCspectra of WNV NS2B–NS3pro(K96A) in the presence of 3 and in the presence of the Bz-nKRR-H inhibitorused in one of the...

Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf

... amixture of annexin A1 and A2 and that the 35 kDa proteins are a mixture of annexin A5 and proteolytic fragments of annexin A1 and annex-in A2. Puriﬁed recombinant annexin A1, annexin A2 and annexin ... show the sites cleaved and the mole-cular masses of the rest of the cleaved peptides. Arrows show theN-termini of the 35 kDa forms of annexin A1 and annexin A2.Role of dicalcin in frog olfactory ... afamily of EF-hand Ca2+-binding proteins, and consists of two S100A11-like regions aligned in sequence. Theamino acid sequences in the N-terminal and the C-ter-minal halves show 58% and 45%...

Báo cáo khoa học: Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin pptx

... loss of enzyme activity through imprinting, immo-bilizing and crosslinking of the native enzyme. Thetemperature stability of the immobilized and CLIPCGTases from A11 and BM at 60 °C and 70 ... to ﬁve cycles of synthesis reactions. Comparison of the products obtained from thenative, immobilized and CD8-imprinted CLIP CGTas-es revealed that the native CGTases from A11 and BM produced ... 7.0–9.0 and 9.0–11.0, respectively. For determining the effect of tem-perature on the enzyme activity, the reactions were per-formed between 30 °C and 80 °C.Effect of pH on native, immobilized and CD8-imprinted...

Báo cáo khoa học: Identiﬁcation of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx

... analyzed using MALDI-TOF MS. Theamount of peptide generated was estimated from theratio of the height of the product peptide peak versusthe sum of the heights of the product peptide and sub-strate ... compilation ª 2005 FEBS of angiotensin I and II and angiotensinogen 1–14 byyeast aspartyl aminopeptidase revealed that the enzymecleaved the shorter peptides at a higher rate.Effect of inhibitors on ... bestatin.Yeast aminopeptidase I and aspartyl aminopepti-dase, both of which belong to the M18 family of metal-loprotease, comprise 12 identical subunits, and theactivity of these enzymes is inhibited...

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... with Hill coefﬁcients of 2.4 and 1.9 and dissoci-ation constants (KdHill) of 4 and 2 lm for hGBP5a ⁄ b and hGBP5ta, respectively. The proteins are very similarin terms of their enzymatic activity, ... enzymatic activity of hGBP5a ⁄ b and hGBP5ta in a concentration-dependentmanner. Various concentrations of puriﬁed hGBP5a ⁄ b and hGBP5ta were incubated with 350 lm of GTP, and aliquots were ... assays,ﬂuorescence titrations and analytical gel ﬁltration.Results and DiscussionHydrolytic activity of hGBP5a/b and hGBP5taIn light of previous observations for hGBP1 and otherlarge GTPases,...

Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"

... detailed analysis of the level of utilization of a MET service over a 24-hour period and found asignificant increase in the number of MET calls around periods of nursing handover and routine nursing ... Department of Intensive Care and Department of Surgery (Melbourne University), Austin Hospital, Melbourne, Australia2Research Nurse. Department of Intensive Care and Department of Surgery (Melbourne ... 08:00–18:00 (47% of calls during 42% of the day), asubstantial proportion of MET calls occur after normal workinghours (53% of calls during 58% of the day), with the peak time of activity occurring...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... some of the key ﬁndings that have led to our current under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance.Structure and function of ... nature of theactive site or the mode of action of glyphosate. A dec-ade later, the crystal structure of EPSPS was deter-mined in complex with S3P and glyphosate [15]. Thecompactness of the liganded ... observed pH dependence of kcat, and explains the dual role of His138 in substrate binding and as a catalytic base.To complete the reaction, attack by the lone pair of the glyphosate nitrogen...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... induced theappearance of an  68-kDa band in wild-type, Y18F,Y29F, Y197F and Y310F tau, this band was notapparent on western blots of lysates of Y394F tau(Fig. 2A). The absence of this larger species ... models of Alzheimer’s disease. Disruption of interactions between Fyn and tau may thus have the potential to protect neurons fromAb-induced neurotoxicity. Here, we investigated tau and Fyn interactions and ... revealeddecreased electrophoretic mobility of tau, with theappearance of an  68-kDa tau species in wild-type and all of the mutant forms of tau except for YallF and Y394F tau (Fig. 3). In contrast...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... Rouvinen1 and Nina Hakulinen11 Department of Chemistry, University of Eastern Finland, Joensuu, Finland2 VTT Technical Research Centre of Finland, Espoo, FinlandKeywordsascomycete; C-terminal plug; ... Waltham, MA, USA). The reactions werestarted by addition of substrate, and the rate of substrateoxidation was measured by monitoring the change in absor-bance over 5 min. All of the measurements ... EU-project ‘Enzymatic tailoring of pro-tein interactions and functionalities in food matrix’,PRO-ENZ (MEST-CT-2005-020924), and from theNational Technology Agency of Finland, Tekes pro-ject 40522...

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