Báo cáo khoa học: Adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases ppt

... quantifi- cation module in the software package. The PCR reactions were performed in a final volume of 10 lL containing 1 lL of cDNA sample, 0.4 lm each primer, and 5 lL of the 2· High Resolution Melting ... open questions. The use of mutant (e.g. deletion and extra copy numbers) and transgenic (RNAi and overexpres- sion) lines will help us to better understand the relationsh...

Ngày tải lên: 06/03/2014, 09:22

... site. A NOESY spectrum of 2 and 3 in the presence of a small quantity of protease revealed an intermolecular cross-peak between the methyl group of 3 and the phthalazine ring of 2,as expected for ... the exception of prolines and a few resi- dues with very broad amide peaks. The resonances of the peptide connecting NS2B and NS3pro appeared at chemical shifts characterist...

Ngày tải lên: 07/03/2014, 02:20

... a mixture of annexin A1 and A2 and that the 35 kDa proteins are a mixture of annexin A5 and proteolytic fragments of annexin A1 and annex- in A2. Purified recombinant annexin A1, annexin A2 and annexin ... show the sites cleaved and the mole- cular masses of the rest of the cleaved peptides. Arrows show the N-termini of the 35 kDa forms of annexin A1 and annexin A2....

Ngày tải lên: 07/03/2014, 05:20

... loss of enzyme activity through imprinting, immo- bilizing and crosslinking of the native enzyme. The temperature stability of the immobilized and CLIP CGTases from A11 and BM at 60 °C and 70 ... to five cycles of synthesis reactions. Comparison of the products obtained from the native, immobilized and CD 8 -imprinted CLIP CGTas- es revealed that the native CGTases from A...

Ngày tải lên: 07/03/2014, 10:20

... analyzed using MALDI-TOF MS. The amount of peptide generated was estimated from the ratio of the height of the product peptide peak versus the sum of the heights of the product peptide and sub- strate ... compilation ª 2005 FEBS of angiotensin I and II and angiotensinogen 1–14 by yeast aspartyl aminopeptidase revealed that the enzyme cleaved the shorter peptides at a higher...

Ngày tải lên: 07/03/2014, 12:20

... with Hill coefficients of 2.4 and 1.9 and dissoci- ation constants (K d Hill ) of 4 and 2 lm for hGBP5a ⁄ b and hGBP5ta, respectively. The proteins are very similar in terms of their enzymatic activity, ... enzymatic activity of hGBP5a ⁄ b and hGBP5ta in a concentration-dependent manner. Various concentrations of purified hGBP5a ⁄ b and hGBP5ta were incubated with 350 lm...

Ngày tải lên: 15/03/2014, 10:20

... detailed analysis of the level of utilization of a MET service over a 24-hour period and found a significant increase in the number of MET calls around periods of nursing handover and routine nursing ... Department of Intensive Care and Department of Surgery (Melbourne University), Austin Hospital, Melbourne, Australia 2 Research Nurse. Department of Intensive Care and...

Ngày tải lên: 25/10/2012, 10:45

... some of the key findings that have led to our current under- standing of the molecular mode of action of glypho- sate and the molecular basis for glyphosate resistance. Structure and function of ... nature of the active site or the mode of action of glyphosate. A dec- ade later, the crystal structure of EPSPS was deter- mined in complex with S3P and glyphosate [15]. The...

Ngày tải lên: 14/02/2014, 14:20

... induced the appearance of an  68-kDa band in wild-type, Y18F, Y29F, Y197F and Y310F tau, this band was not apparent on western blots of lysates of Y394F tau (Fig. 2A). The absence of this larger species ... models of Alzheimer’s disease. Disruption of interactions between Fyn and tau may thus have the potential to protect neurons from Ab-induced neurotoxicity. Here, we inve...

Ngày tải lên: 14/02/2014, 14:20

... Rouvinen 1 and Nina Hakulinen 1 1 Department of Chemistry, University of Eastern Finland, Joensuu, Finland 2 VTT Technical Research Centre of Finland, Espoo, Finland Keywords ascomycete; C-terminal plug; ... Waltham, MA, USA). The reactions were started by addition of substrate, and the rate of substrate oxidation was measured by monitoring the change in absor- bance over 5 mi...

Ngày tải lên: 14/02/2014, 18:20