Báo cáo khoa học: Entamoeba histolytica TATA-box binding protein binds to different TATA variants in vitro ppt
Báo cáo khoa học: cAMP response element-binding protein (CREB) is imported into mitochondria and promotes protein synthesis docx
... PKA in providing a regulatorymechanism in the expression of mitochondriallyencoded proteins of the respiratory chain prompted us to investigate the mitochondrial import of the CREB protein, ... place in vivo in the cytosolbefore its import into mitochondria, and ⁄ or after it isimported into mitochondria. PKA is, in fact, present in various subcellular regions, including the cytosol ... nuclear-encoded mitochondrial matrix-targeted proteins[26,28]. The inhibition of both mitochondrial surface binding and import into mitochondria of radioactiveCREB by the antibody against Tom20 shows,...
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Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx
... interacts withF-actin via b-catenin and a-catenin; b-catenin binds to cadherin and a-catenin, which in turn interacts withF-actin (Fig. 3A) [24]. Thus b-catenin plays a pivotalrole in the regulation ... synchronous increases in intracellular [Ca2+], as described below.Synchronous increases in intracellular [Ca2+] anddisassembly of cadherin–catenin complexesThe cytoplasmic domain of cadherin interacts ... directly tyrosine-phosphorylated by the nonre-ceptor protein tyrosine kinase proline-rich tyrosinekinase 2 (Pyk2) [26,27], or is indirectly tyrosine-phosphorylated by Src family kinase, which...
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Tài liệu Báo cáo khoa học: Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits ppt
... oneshown in A). (C) GST fusion proteins containing the C termini of a, b,andcENaC were phosphorylated by fraction 76. Autoradiogram andCoomassie blue staining are shown.Fig. 2. Binding of kinases to ... byphosphorimaging of the band corresponding to the full-length protein, and normalizing to the Coomassie bluestaining of the same band.)It was next demonstrated that the kinase eluted in fraction ... observed both in vitro using glutathi-one-S-transferase–ENaC fusion proteins and in vivo in ENaC-expressing Xenopus oocytes. The c subunit is weaklyphosphorylated by this protein kinase on another...
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Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc
... amino acid side chains.Amino acid residues interacting with the guaninemoiety of GDP in EhPGKThe amino acid residues known to interact with theadenine moiety in the ADP ⁄ ATP -binding site in ... (red) obtained usingMOE software. (B) Close up of the purine ring binding site in theC-terminal domain. Lines indicate hydrogen bonds interactions. Let-tering in red corresponds to EhPGK, ... C).The corresponding reported amino acid residuesinvolved in adenine binding in the other crystallizedproteins, as well as in the EhPGK identified in thepredicted model, were totally conserved...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... phosphatidyl-ethanolamine -binding protein; PG, phosphatidylglycerol; PS, phosphatidylserine; PtdIns, phosphatidylinositol; PtdIns(3)P, phosphatidylinositol3-phosphate; PtdIns(4)P, phosphatidylinositol 4-phosphate; ... that theN-terminal segment (Met1–His18) in its carboxypeptidase Y -binding sitesis involved in the specific and efficient binding to anionic phospholipidmembranes. The binding of IC to cellular ... a member of the phos-phatidylethanolamine -binding protein family. The binding of IC to phos-pholipid membranes was first analyzed using a liposome -binding assay andby surface plasmon resonance...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... times. To analyze the RNA synthes-ized in one round of synthesis, we performed RdRpassays in the presence of heparin in order to preventreinitiation. In a first step we determined the heparinconcentration ... posit-ive-stranded RNA genome and belongs to the Flavivir-idae family. The RNA contains a large open readingframe that encodes a polyprotein which is cleaved into10 viral proteins: C, E1, E2, p7, NS2, NS3, ... very limited role in binding to the viral polymerase. On thecontrary, deletions of sequences in the 5¢-end of this fragment greatlyimpaired both RNA synthesis and RNA binding. Our results strongly...
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Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... conformation in NBD2 that is nonpermissive for ATP binding. Theresulting ADP -binding state of TAP2 and the C-terminaltail of NBD1 allow binding of ATP to TAP1. Peptide binding leads to a change in the ... ADP -binding state of TAP2allows binding of ATP to TAP1. We hypothesize that peptide binding to TAP leads to transient change in the conformation of NBD2 that istransduced to the C-terminal ... NBDs have distinct intrinsic nucleotide binding properties. In the resting state of TAP, the NBD1has a much higher binding activity for ATP than the NBD2,while the binding of ADP to the two NBDs...
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Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitrificans Surf1 proteins doc
... part of the proteinsled to impaired heme a binding compared with wild-type, further emphasizing that heme binding must bean important physiological role of the protein, at least in the bacterial ... heme moiety.Interestingly, in the tryptophan to phenyalaninemutant in transmembrane helix II (W200F in Surf1c,W2009F in Surf1q), heme o is the predominantheme cofactor, indicating that this ... each Surf1 protein confirmed their requirement forheme binding. Interestingly the mutation of a tryptophan residue in trans-membrane helix II (W200 in Surf1c and W209 in Surf1q) led to a drasticswitch...
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Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx
... con-sists of 226 amino acids, binds to both ssDNA anddsDNA, and catalyzes HP in an ATP-independentmanner in vitro [15,17,18]. In addition to Mhr1, other proteins that promoteHP in an ATP-independent ... examined the DNA -binding activity ofMhr1 in the absence of Mg2+, because the DNA- binding activities of other HP proteins are oftenincreased by the addition of Mg2+ in vitro [31,32]. In the ... 1A) and 11 Tyrresidues. Therefore, we examined the binding of Mhr1 to DNA by measuring changes in the fluorescencespectra of Mhr1 after binding. To distinguish thefluorescence of Trp from that...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
... fusion protein was correctly expressed in E. coli.DNA -binding characteristics of the DA-HbpR protein EMSA performed on the wild-type HbpR -binding siteand increasing amounts of DA-HbpR or HbpR in fusion ... direct palin-dromic sequences, decreased the binding affinity. Footprints of HbpR onmutant operator fragments showed that a partial loss of binding contactsoccurs, suggesting that the binding of ... protomer contacts theDNA.The objectives of this work were to identify criticalmotifs in the binding site for HbpR and to examinethe necessity of the sensing A-domain of HbpR forDNA binding. ...
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