Báo cáo khoa học: Entamoeba histolytica TATA-box binding protein binds to different TATA variants in vitro ppt

... PKA in providing a regulatory mechanism in the expression of mitochondrially encoded proteins of the respiratory chain prompted us to investigate the mitochondrial import of the CREB protein, ... place in vivo in the cytosol before its import into mitochondria, and ⁄ or after it is imported into mitochondria. PKA is, in fact, present in various subcellular regions, including th...

Ngày tải lên: 07/03/2014, 02:20

... interacts with F-actin via b-catenin and a-catenin; b-catenin binds to cadherin and a-catenin, which in turn interacts with F-actin (Fig. 3A) [24]. Thus b-catenin plays a pivotal role in the regulation ... synchronous increases in intracellular [Ca 2+ ], as described below. Synchronous increases in intracellular [Ca 2+ ] and disassembly of cadherin–catenin complexes The cytoplasmi...

Ngày tải lên: 16/02/2014, 09:20

... one shown in A). (C) GST fusion proteins containing the C termini of a, b,andcENaC were phosphorylated by fraction 76. Autoradiogram and Coomassie blue staining are shown. Fig. 2. Binding of kinases to ... by phosphorimaging of the band corresponding to the full- length protein, and normalizing to the Coomassie blue staining of the same band.) It was next demonstrated that the kina...

Ngày tải lên: 21/02/2014, 03:20

... amino acid side chains. Amino acid residues interacting with the guanine moiety of GDP in EhPGK The amino acid residues known to interact with the adenine moiety in the ADP ⁄ ATP -binding site in ... (red) obtained using MOE software. (B) Close up of the purine ring binding site in the C-terminal domain. Lines indicate hydrogen bonds interactions. Let- tering in red corresponds...

Ngày tải lên: 16/03/2014, 01:20

... phosphatidyl- ethanolamine -binding protein; PG, phosphatidylglycerol; PS, phosphatidylserine; PtdIns, phosphatidylinositol; PtdIns(3)P, phosphatidylinositol 3-phosphate; PtdIns(4)P, phosphatidylinositol 4-phosphate; ... that the N-terminal segment (Met1–His18) in its carboxypeptidase Y -binding sites is involved in the specific and efficient binding to anionic phospholipid membranes...

Ngày tải lên: 19/02/2014, 05:20

... times. To analyze the RNA synthes- ized in one round of synthesis, we performed RdRp assays in the presence of heparin in order to prevent reinitiation. In a first step we determined the heparin concentration ... posit- ive-stranded RNA genome and belongs to the Flavivir- idae family. The RNA contains a large open reading frame that encodes a polyprotein which is cleaved into 10...

Ngày tải lên: 20/02/2014, 01:20

... conformation in NBD2 that is nonpermissive for ATP binding. The resulting ADP -binding state of TAP2 and the C-terminal tail of NBD1 allow binding of ATP to TAP1. Peptide binding leads to a change in the ... ADP -binding state of TAP2 allows binding of ATP to TAP1. We hypothesize that peptide binding to TAP leads to transient change in the conformation of NBD2 that...

Ngày tải lên: 20/02/2014, 02:21

... part of the proteins led to impaired heme a binding compared with wild- type, further emphasizing that heme binding must be an important physiological role of the protein, at least in the bacterial ... heme moiety. Interestingly, in the tryptophan to phenyalanine mutant in transmembrane helix II (W200F in Surf1c, W2009F in Surf1q), heme o is the predominant heme cofactor, i...

Ngày tải lên: 06/03/2014, 00:21

... con- sists of 226 amino acids, binds to both ssDNA and dsDNA, and catalyzes HP in an ATP-independent manner in vitro [15,17,18]. In addition to Mhr1, other proteins that promote HP in an ATP-independent ... examined the DNA -binding activity of Mhr1 in the absence of Mg 2+ , because the DNA- binding activities of other HP proteins are often increased by the addition of Mg...

Ngày tải lên: 06/03/2014, 09:22

... fusion protein was correctly expressed in E. coli. DNA -binding characteristics of the DA-HbpR protein EMSA performed on the wild-type HbpR -binding site and increasing amounts of DA-HbpR or HbpR in fusion ... direct palin- dromic sequences, decreased the binding affinity. Footprints of HbpR on mutant operator fragments showed that a partial loss of binding contacts occurs, sugge...

Ngày tải lên: 07/03/2014, 17:20