Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt

... DEC1. Determination of the binding domain of DEC1 to BMAL1 The interaction of DEC1 and BMAL1 was previously demonstrated by a yeast two- hybrid assay [ 7], and this interaction may be involved in the suppressive ... uto- radiography. Results Deletion analysis of DEC1 on its suppressive activity in the presence of CLOCK and BMAL1 Using deletion analyses, two region...

Ngày tải lên: 19/02/2014, 16:20

... 2010) doi:10.1111/j.1742-4658.2010.07835.x Fibronectin-binding proteins A and B are multifunctional LPXTG staphy- lococcal adhesins, comprising an N-terminal region that binds fibrinogen and elastin, and a C-terminal domain that interacts ... fibronectin. The C-terminal domain of fibronectin-binding protein A is organized into 11 tandem repeats, six of which bind the ligand with high a...

Ngày tải lên: 06/03/2014, 22:21

... misfolded proteins and con- tain components of the protein degradation and refold- ing machinery, including proteasome complexes and members of the Hsc70 and Hsp70 families of proteins [26,27]. ... ratio of intensity of the myc–GALE to tubulin bands in the western blot and normalized by setting the amount of GALE in ldlD(myc–GALE) wt #11 as 1.0. The western blot for...

Ngày tải lên: 07/03/2014, 00:20

... at some point in the reaction. Modest changes in K m,ATP are seen in H44Y and R68C. That H44 in uences the binding of both substrates suggests that the binding sites are probably close in space. R68 ... change in the enzyme, which creates a functional binding site for galactose. Identifying the nature of this change and the residues involved in transmitting informatio...

Ngày tải lên: 08/03/2014, 02:20

... initiate a bioinformatic anal- ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding. The amino acid sequence of Zm-p60.1 ... structural and catalytic prop- erties of the mutants, and simulations of the sub- strate–enzyme interactions of the wild-type and one of the mutants. The resul...

Ngày tải lên: 16/03/2014, 04:20

... mechanisms of ligand binding, signaling, and regulation within the superfamily of G-protein-coupled receptors: molecular modeling and C. Klammt et al. Ligand binding of cell-free endothelin B receptor FEBS ... (E1–3) and three cytoplasmic (C1–3) loops. In addition, GPCRs contain a more or less extended N-terminal domain and C-terminal domain, which are both often involved eith...

Ngày tải lên: 16/03/2014, 10:20

... sites of Mglu and Bacillus glutaminase in the presence and absence of ligands. Backbone atoms of the seven conserved amino acid residues and the three conserved tyrosine residues in Mglu and Bacillus ... amino acid residues of Mglu and Bacillus glutaminases induced by ligand binding. Displacements of the conserved amino acid residues in Table 2 are indicated by circl...

Ngày tải lên: 06/03/2014, 09:22

... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig. 2). PCR reactions performed in ... FEBS Results Expression and purification of E. coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E. coli Pta, t...

Ngày tải lên: 06/03/2014, 11:20

... to be linked to early stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actin bundles regulator fascin 1 [27] and discordant changes of two calcium-dependent, ... dopamine treatment, overexpres- sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase (GST)p, Ran 1 binding prot...

Ngày tải lên: 15/02/2014, 01:20

... ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been described from all three domains of life [1,2], and usually ... authentic C-terminal Gly145. Expression and purification Recombinant protein was produced by cultivating the expression strain in shaking flasks containing LB medium supplem...

Ngày tải lên: 18/02/2014, 06:20