Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt
Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... DEC1.Determination of the binding domain of DEC1 to BMAL1The interaction of DEC1 and BMAL1 was previouslydemonstrated by a yeast two- hybrid assay [ 7], and thisinteraction may be involved in the suppressive ... uto-radiography.Results Deletion analysis of DEC1 on its suppressive activity in the presence of CLOCK and BMAL1Using deletion analyses, two regions in the proteinproduct of Dec1 have been identified ... estern blot analysis (Fig. 5 ). These r esults indicate that the bHLHregion, including His57 and Arg65, is responsible for t heE-box binding.Determination of the region in BMAL1 for bindingto...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... 2010)doi:10.1111/j.1742-4658.2010.07835.xFibronectin-binding proteins A and B are multifunctional LPXTG staphy-lococcal adhesins, comprising an N-terminal region that binds fibrinogen and elastin, and a C-terminal domain that interacts ... fibronectin. TheC-terminal domain of fibronectin-binding protein A is organized into 11tandem repeats, six of which bind the ligand with high affinity; other sitesbind more weakly. Fibronectin-binding ... of struc-tural and functional features of the Fn-binding moie-ties of FnBPA and FnBPB.ResultsHigh-affinity binding sites for full-length Fn and its N-terminal fragment in FnBPA and FnBPBIn...
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Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
... misfolded proteins and con-tain components of the protein degradation and refold-ing machinery, including proteasome complexes and members of the Hsc70 and Hsp70 families of proteins[26,27]. ... ratio of intensity of the myc–GALE to tubulin bands in the western blot and normalized by setting the amount of GALE in ldlD(myc–GALE)wt #11 as 1.0. The western blot for determining the protein ... determined using UDP-galac-tose as previously described [20,32]. The relative proteinabundance in each lysate was calculated by measuring theintensity of the individual GALE and tubulin bands in the...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
... at some point in thereaction. Modest changes in Km,ATPare seen in H44Y and R68C. That H44 in uences the binding of both substratessuggests that the binding sites are probably close in space.R68 ... change in the enzyme, which creates a functional binding site for galactose. Identifying the nature of this change and the residues involved in transmittinginformation through the protein will ... substratebinding sites. The data represents the first step in the analysis of the metabolic control of flux through the Leloirpathway. Analysis of the galactokinase, its mutants, and theother enzymes of...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... initiate a bioinformatic anal-ysis of plant b-glucosidases to obtain insights into theevolution of the molecular sites involved in the two modes of aglycone binding.The amino acid sequence of Zm-p60.1 ... structural and catalytic prop-erties of the mutants, and simulations of the sub-strate–enzyme interactions of the wild-type and one of the mutants. The results provide indications of thenative ... Waals interactionswith edges of F193, F200, and F461 located oppositeW373 in a slot-like aglycone-binding site [13,15]. In addition, kinetic analysis and computer simulations of F193I ⁄ Y ⁄ W mutants...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... mechanisms of ligandbinding, signaling, and regulation within the superfamily of G-protein-coupled receptors: molecular modeling and C. Klammt et al. Ligand binding of cell-free endothelin B receptorFEBS ... (E1–3) and three cytoplasmic (C1–3) loops. In addition, GPCRs contain a more or less extendedN-terminal domain and C-terminal domain, which areboth often involved either in ligand binding or ... 4B).Localization of the ET-1-binding site In order to determine the ETB region that is essentialfor binding of ET-1, a series of eight plasmids codingfor terminally truncated ETB fragments and contain-ing...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... sites of Mglu and Bacillus glutaminase in the presence and absence of ligands. Backbone atoms of the seven conservedamino acid residues and the three conserved tyrosine residues in Mglu and Bacillus ... amino acid residues of Mglu and Bacillus glutaminases induced by ligand binding.Displacements of the conserved amino acid residues in Table 2 areindicated by circles. (A) Backbone atoms of ... smallerstructural changes of the protein part in Mglu. A comparison of the sub-strate-binding pocket of Mglu, which is highly specific for l-glutamine, withthat of Erwinia carotovora asparaginase, which...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... containingthe last 326 amino acids in the case of Pta-F1, the last 352amino acids in the case of Pta-F2, and the last 470 aminoacids in the case of Pta-F3 (Fig. 2).PCR reactions performed in ... FEBSResultsExpression and purification of E. coli Pta and truncated Ptas containing the C-terminal endBy analysis of the protein domain architecture of E. coli Pta, three conserved domains can be detected[Conserved ... islocated in the C-terminal domain, the E. coli PtaN-terminal domain is involved in stabilization of thehexameric native structure, in expression of the max-imum catalytic activity, and in allosteric...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... to be linked toearly stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actinbundles regulator fascin 1 [27] and discordant changes of two calcium-dependent, ... dopamine treatment, overexpres-sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding pro-tein], ... physical interactions between theidentified proteins, aiming to validate the body of theresults as a whole. Unexpectedly, all proteins wereincluded in two different networks and proteinsresponding...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... ligand binding, and the role of storage proteins, the cupins constitute one of the mostdiverse superfamilies of proteins known. They havebeen described from all three domains of life [1,2], and usually ... authenticC-terminal Gly145.Expression and purificationRecombinant protein was produced by cultivating theexpression strain in shaking flasks containing LB mediumsupplemented with 100 lgÆ mL)1ampicillin. ... Straganz and Bernd NidetzkyInstitute of Biotechnology and Biochemical Engineering, Graz University of Technology, AustriaIntroduction In terms of their physiological functions, which includeenzymatic...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ