Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc

... Cytosolic 5¢-nucleotidase II mechanism (Eur. J. Biochem. 271) 4891 Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily Simone Allegrini 1, *, Andrea ... a stepwise manner, using an i ncreasing concentration of acetonitrile in the elution solution, and loaded directly on the MALDI target by using the d...

Ngày tải lên: 16/03/2014, 18:20

... negative control. The company has an Animal Assurance number filed with USDA and an approved animal protocol through its institutional animal care and use committee. Protein determinations The protein ... predigested with the same enzymes, to generate pQE30-rocF. The construct was confirmed by sequencing, restriction enzyme digestion, and mini-protein expression analyses (data n...

Ngày tải lên: 16/03/2014, 16:20

... binds to actin and inhibits contraction. Upon muscle stimula- tion, Ca 2+ binds to TnC and induces the release of the inhibition by TnI, resulting in muscle contraction. To understand the molecular ... contraction through the activating mechanisms that involve the region spanning from the structural TnC- binding site to the inhibitory region of TnI. Together with the o...

Ngày tải lên: 20/02/2014, 01:20

... sharpened at the position of the second signal at [sugar] ⁄ [EW29Ch] = 3–4 and the resonance signal of the side-chain NH of Trp33 appeared at the posi- tion of the second signal corresponding to the bound state ... signals shifted to the position of the second signal correspond- ing to the bound state during titration ([sugar] ⁄ [EW29Ch] = 0.5–3) and the resonance...

Ngày tải lên: 23/03/2014, 04:21

... adjacent to the p-nitro- phenyl group. This was the same in the case of a comparison of 1 and 3. Furthermore, the enzyme also catalysed a transglycosylation on 1 and converted it into GlcNAc b1-3Galb1-4GlcNAcb1-3Galb1-4GlcNAcb-pNP ... serum. They were further converted into 2 and 4 utilizing b4GalT from bovine milk (Fig. 2A,B). The enzyme efficiently catalysed the transfe...

Ngày tải lên: 31/03/2014, 07:20

... kết cấu thanh chịu nén bởi các lực bảo to n và không bảo to n theo tiêu chuẩn ổn định dưới dạng động lực học. Đây là cơ sở để áp dụng phương pháp MTĐCĐL vào các bài to n ổn định hệ thanh phức ...             tbiatibaiti exYexYexYtxy   ,  Như vậy, khi 0  b , biên độ chuyển vị của thanh sẽ tăng theo thời gian, do đó, theo tiêu chuẩn động lực học, thanh công xôn ch...

Ngày tải lên: 23/03/2014, 06:20

... and stored at )70 °C. Skin library construction and screening Total RNA from Atlantic snailfish skin tissue was isolated using TRIzolÒ reagent (Invitrogen Canada Inc, Burlington, ON, Canada) and ... snailfish and the liver is an ancillary site of expression for contributing supple- mentary protection. Snailfish may rely more on skin (and its AFP content) to provide the primary barrier...

Ngày tải lên: 20/02/2014, 02:22

... computationally prohibitive, since the tagset contains only four ele- 8 The transitivity dependency is conditioned on two tags, while all others on only one. These five contextual tags are de- faulted to OTHER when dependency ... of the Markov chain only depends on the preceding tag and the local observation . However, in order to incorporate more than one label dependency...

Ngày tải lên: 20/02/2014, 16:20

... Signal transducer and activator of transcription 5 activation is sufficient to drive transcriptional induction of cyclin D2 gene and proliferation of rat pancreatic beta-cells. Mol Endocrinol ... 2004) doi:10.1111/j.1432-1033.2004.04391.x The leptin receptor (LEPR) is a class I cytokine receptor signalling via both the janus kinase ⁄ signal transducer and activator of transcription (JAK...

Ngày tải lên: 07/03/2014, 16:20

... for the lamellar structure the appropriate fractions between the surfactant and MO are OG/MO ¼ 1:16or DM/MO ¼ 1 : 21. Note however, that these ratios refer to total concentrations and not to the ... need to remove them from their native membrane environment, and solubilize them in mild detergent micelles. The solubilization process may lead to denaturation of the proteins,...

Ngày tải lên: 17/03/2014, 09:20