Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf

... the 5¢- and 3¢-UTR are underlined and the 13 repeats with a consensus of CCAATGATGATCCAAGAAATCCACACTACAG (31 bp) in the 3¢-UTR are numbered and distinguished from each other by alternate highlighting in ... insertion of 12 repeats with a consensus of CCAATGATGATCCAAGAAATCCACACTACAG (31 bp) in the 3¢-UTR of the cDNA sequence (Fig. 1). A TATA box was identified 28 bp upstre...

Ngày tải lên: 07/03/2014, 16:20

... Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase Christiane Stuhlfelder, Martin J. Mueller and Heribert Warzecha Lehrstuhl fu ¨ r Pharmazeutische ... Intra- cellular s eparation of MeJA synthesis and hydrolysis would be one way to avoid a treadmill situation, yet analysis of tomato MJE and Arabidopsis JMT reveals no...

Ngày tải lên: 16/03/2014, 18:20

... bp R5¢-GGCTTTGGCCATACGCATAC-3¢ 3081 P a 5¢-ACGGCCAGCGGCTCGCA-3¢ 3037 FK long F 5¢-GCAGGACGTGCTGAGGAACT-3¢ 2865 64 bp R5¢-CAGTCTGCGGGCATTCTGT-3¢ 2911 P a 5¢-CCACAACGGGCAACCGAGCG-3¢ 2889 PP F 5¢-AGCTGGGCTTACAATCCATAGCT-3¢ ... needed. Acknowledgements We thank Tuula Kallioinen and Sirkka-Liisa Kauranen for skilled technical assistance in molecular biology, and Kati Vena ¨ la ¨ inen and...

Ngày tải lên: 30/03/2014, 13:20

... C)AATTTC-3¢ PsCBL (degenerate forward) 45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse) 55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward) 65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR ... T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward) 25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse) 35¢-CTTAT(C ⁄ G)AACAAGGAA (A...

Ngày tải lên: 19/02/2014, 07:20

... Japan) and reared on a commercial diet at 15 °C. Cloning of HSF cDNA A random primed kZAPII cDNA library was constructed by using a kZapII predigested EcoRI/calf intestinal alkaline phosphatase-treated ... PCR was performed with the M13 forward primer (5¢-CCCAGTCACGACGTTGTAAAA CG-3¢)asasenseprimerandHSF1-specific primers (for HSF 1a, 5¢-GAAGCAGCTTGTCCAGTACACTAA-3¢;for HSF1b,5¢-...

Ngày tải lên: 19/02/2014, 12:20

... and XhoI ML3p A- chain 5¢- GATATA CATATG TACCGTCGTATTAGCCTTCGTGTCACGGAT -3¢ 5¢- CACAC GAATTC TTATTAAGAAGAAGAAGAACGGTCCCTGCATAC -3¢ NdeI and EcoRI ML3.1p A- chain 5¢- GATATA CATATG TACGAGCGTCTTCGTCTTCGTGTTACGCATC -3¢ 5¢- CACAC GAATTC TTATTAAGAAGAAGAAGAACGGTCCCTGCATAC -3¢ NdeI ... and XhoI ML2p A- chain 5¢- GATATA CATATG TACGAGCGTCTTCGTCTTCGTGTTACGCATC -3¢ 5¢- CACAC CTCGAG TTATTAAGAAGA...

Ngày tải lên: 07/03/2014, 15:20

... vector, and sequenced on both strands. The cloned cDNA was 1370 bp long and contained an ORF of 1257 bp, encoding a protein of 418 amino a cids with a calculated molecular mass of 46 751 Da. The ... Beccari 4 , Maria Agnese Della Fazia 4 and Giuseppe Servillo 4 1 Department of Internal Medicine, University of Perugia, Italy; 2 Department of Pharmacological Sciences...

Ngày tải lên: 08/03/2014, 16:20

... EMBL database and are available under accession numbers AJ309822 and AJ309823, respectively. DpAR1 and DpAR2 contain 948 bp long ORFs encoding 315 amino acids of a calculated molecular mass 34 ... (P23901); Avena, Avena fatua (S61421); Sesbania, Sesbania rostrata (CAA11226); Oryza, Oryza sativa (AAK52545); Medicago, M. sativa (X97606). AR proteins from mammals were used as the out...

Ngày tải lên: 18/03/2014, 01:20

... 2004 Cloning, over -expression, purification and characterization of Plasmodium falciparum enolase Ipsita Pal-Bhowmick, K. Sadagopan, Hardeep K. Vora, Alfica Sehgal*, Shobhona Sharma and Gotam K. Jarori Department ... falciparum NF54 LGANAILAISMAVCRAGAAANKVSLYKYLAQLAGKKSDQMVLPVPCLNVINGGSHAGNKL 171 P. falciparum K1 LGANAILAISMAVCRAGAAPNKVSLYKYLAQLAGKKSDQMVLPVPCLNVINGGSHAGNKL 171 P. yoel...

Ngày tải lên: 16/03/2014, 18:20

... for ACS4 and MTE-I in the hormonal regulation of steroidogenesis as a new pathway of arachidonic acid release different from the classical phospho- lipase A 2 cascade. Abbreviations AA, arachidonic ... thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoA to the nonesterified fatty acid and CoA [19] and that they can release AA from the arachidonoyl-...

Ngày tải lên: 16/03/2014, 18:20