Báo cáo khóa học: Functional characterization of the evoluti

Báo cáo khóa học: Functional characterization of the evolutionarily divergent fern plastocyanin potx

... assuming that the unusual s urface charge distribution of fern Pc impairs the interaction. Fern PSI behaves in a similar way as spinach PSI in reaction with other Pcs. The reactivity of fern Pc towards ... it is thus of interest to analyse the functional behaviour of fern Pc as compared to other plant Pcs. In this study, Pcs from the fern genera Nephrolepsis and...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Functional characterization of the maltose ATP-binding-cassette transporter of Salmonella typhimurium by means of monoclonal antibodies directed against the MalK subunit pot

... 2F9 are located on the same site but at the bottom part of the C-terminal domain. The only moderate effect of MalT on binding of 2F9 argues against these residues being part of the MalT–MalK interaction ... correspond to the position of the indicated residue in the E. coli MalK protein (see Fig. 1A). Please note that in Fig. 7B, the epitope of 5B5 is located on...

Ngày tải lên : 31/03/2014, 09:20

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Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the ﬁrst polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

... histidine boxes in the desaturase (C-terminal) domain. The third histidine box has the QX 2 HH instead of the HX 2 HH motif present in other types of desaturases [18]. The genome projects of trypanosomatids ... mammals, so the biosynthesis of PUFAs goes through the desaturation and elongation of essential FAs taken from the diet, linoleic and a-linolenic (18:3 n-3) a...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... for future functional studies of Nog o-A. On the other hand, the recent identiﬁcation of Nogo-40 as a potent NgR antagonist suggests a promising starting point for the design of potential therapeutic ... Zn 2+ . Furthermore, the d etermination of the Nogo-40 solution structure offers a starting point for further understanding the interaction between NgR and Nogo-40, and...

Ngày tải lên : 16/03/2014, 16:20

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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... superoxide in the course of the reaction. The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c. This was vali- dated by adding a total of 2.5 U of commercial ... (1971) The kinetics and mechanisms of the reactions of iron(III) with b-diketones. The formation of monoacetylacetoatoiron(III) and the effect of co...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... K d is the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement ... and ﬁtting to the equation: k obs ¼ k 1 ½Eþk À1 : Measurement of the dissociation constant, K d P of the ribozyme–product complex The equilibrium di...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala ... increase in the mutant mice, the disruption of DEC1 might be compensated by DEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The supp...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal structure of the Ydc2–junction complex. In this study the function of several ... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pai...

Ngày tải lên : 20/02/2014, 11:20

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Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx

... either component of the import machinery significantly decreased the amount of perox- ins of the importomer pulled down by the AAA perox- ins, whereas the amount of components of the AAA complex ... data not shown), thereby con- firming the specifity of the isolation. Taken together, these data indicate a close association of the mem- brane-bound AAA complex and...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and W373K mutants was found to be irreversible (Fig. S3). The propensity of the wild-type and each of the single ... introducing features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic prop- erties of the mutants, and s...

Ngày tải lên : 16/03/2014, 04:20

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