Báo cáo khoa học: Leadzyme formed in vivo interferes with tobacco mosaic virus infection in Nicotiana tabacum potx

... removal of the N-terminal helices 1 and 2 while leaving the interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein–protein or/and protein–DNA interactions. The crystal ... recombination in mtDNA inheritance in S. cerevisiae showed that budding cells were enriched in linear monomers of mtDNA. It was proposed that in addition to a role in initiating a r...

Ngày tải lên: 20/02/2014, 11:20

... the two other remaining units. Approval of the ethics committee was obtained; informed consent was waived. A surgical ICU-independent clinical pharmacist with experi- ence in medication errors ... the prescribing or monitoring of a drug); for example, an order written for aminoglycosides, without ordering a drug plasma level, or without a route of administration. Minor MPE: minor medicat...

Ngày tải lên: 25/10/2012, 10:39

... interacts with F-actin via b-catenin and a-catenin; b-catenin binds to cadherin and a-catenin, which in turn interacts with F-actin (Fig. 3A) [24]. Thus b-catenin plays a pivotal role in the regulation ... synchronous increases in intracellular [Ca 2+ ], as described below. Synchronous increases in intracellular [Ca 2+ ] and disassembly of cadherin–catenin complexes The cytoplasmi...

Ngày tải lên: 16/02/2014, 09:20

... recombinant protein C preparations were determined by SDS-PAGE followed by silver staining. Preparation of mouse activated protein C Incubation of mouse protein C with human or bovine thrombin using ... folded membrane-binding domain [9,10]. This Ca 2+ binding requires the presence of Gla residues. The Gla domains within the protein C family comprise 44 amino acids and contain between nine...

Ngày tải lên: 18/02/2014, 06:20

... MS. During primary turnover, the homocysteine-binding domain (dot- ted barrel) and the CH 3 -H 4 -folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark ... Consistent with this, the crystal structure of the cobalamin-binding domain Homocysteine Methionine Co Methylcob(III)alamin Cob(I)alamin Primary turnover cycle H CH 3 H 4 folate AdoHyc Cob...

Ngày tải lên: 18/02/2014, 08:20

... decapeptides. In addition to the antisera r-anti-l (made in rat) and gp-anti-dW4 (produced in guinea pig), two antisera discriminating CHH isomers (gp-anti-pQl made in guinea pig and rb-anti- pQd in rabbit) ... Universite ´ Paris, France Introduction Modification of the chirality of a single aminoacyl resi- due within a peptide chain is a subtle and intriguing mechanism that remains...

Ngày tải lên: 18/02/2014, 11:20

... different packing configurations can be obtained within one preparation. AFM images allow the differences in protein packing and interaction within the membrane to be visualized. Within the packed ... energy- transducing network. Protein packing may play a determining role in the formation of functional photosynthetic domains and membrane curvature. To further investigate in detail the p...

Ngày tải lên: 18/02/2014, 18:20

... pMOWS, containing HA-EpoR cDNA, was introduced into BaF3 cells by retro- viral transduction. Cell lines stably expressing HA-EpoR (BaF3-HA-EpoR) were selected and maintained in RPMI 1640 (Invitrogen, ... error reduction is achieved by using housekeeping proteins as normalizers or by adding puri- fied proteins in immunoprecipitations as calibrators in combination with criteria-based nor...

Ngày tải lên: 19/02/2014, 07:20

... spermidine or aminopropylcadaverine [13,14]. In fact, when polyamine-depleted cultures of T. cruzi epi- mastigotes in synthetic media were supplemented with putrescine, together with cyclohexylamine ... to a-amanitin of the transcription of arginine decarboxylase (ADC) and cruzipain genes in transformed Trypano- soma cruzi. Permeable parasites were preincubated at 0 °C for 5 min in...

Ngày tải lên: 19/02/2014, 07:20

... binding of ACP to the docking site followed by a conformational change in ACP proposed to inject the acyl chain into the sub- strate-binding pocket [32]. If a similar mechanism is involved in ... inspected individually by eye in the graphics program O [40]. Additional refine- ment was performed in cns using maximum likelihood refinement on amplitudes with simulated annealing and restr...

Ngày tải lên: 19/02/2014, 08:20