Báo cáo khoa học: Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus An intrinsically stable cage-like structure endowed with enhanced stability potx

... 2006 FEBS Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus An intrinsically stable cage-like structure endowed with enhanced stability Stefano Franceschini*, ... exploited during the purification of Dps proteins. To investigate Dps ther- mostability, a protein from the thermophilic cyano- bacterium The...

Ngày tải lên: 16/03/2014, 12:20

... coli chaperone proteins [25–27], and then purified without the use of denaturants. The proteins thus obtained were studied with CD and NMR spectroscopy. Our results show that the putative C-terminal domain ... purification and refolding methods that were successful with the other species. Considering that the sequence homology among fish species is 60% among the PrP1 proteins, 50...

Ngày tải lên: 30/03/2014, 04:20

... synthes- ized from T. pseudonana RNA using the SuperScript TM III RNase H – Reverse Transcriptase (Invitrogen) or the Enhanced Avian Reverse Transcriptase (Sigma) and gene specific primers pairs ... yield the plasmids pYDESA, pYDESB, pY- DESI, pYDESO, and pYDESK. The fidelity of the cloned PCR product was checked by sequencing. The vectors con- taining the T. pseudonana sequen...

Ngày tải lên: 20/02/2014, 01:20

... induction. Lane 2: after IPTG induction for 3 h. Lane 3: the supernatant after sonication. Lane 4: the unbound fraction on the GST affinity resin. Lane 5: the purified GST–Lys33GP eluted from the GST resin. ... of the peptide to I 2 ) to remove the Acm protecting group and allow the formation of another disulfide bond (Cys18 and Cys26) [36]. The two disulfide bonds were then c...

Ngày tải lên: 15/03/2014, 09:20

... reflect the faster reaction of nitrite with deoxy hemes in the R structure than in the T structure [19,20]. Thus, the reaction rate was not maximal at the start of the reaction, where the concen- tration ... was transferred to the tonometer and equilibrated for 1 h in the shaking tonometer to ensure full equilibration of the Hb to the gas atmosphere of the system....

Ngày tải lên: 16/03/2014, 06:20

... the characterization of these structures is therefore of value. GAGs are not primary gene products and therefore their analysis cannot rely on genomic approaches; structural analysis requires their isolation ... for 24 h at 65 °C. The GAGs were pre- cipitated from the soluble fraction by the addition of 4 vol. ethanol, and the solution was cooled to 6 °C and allowed to stand overn...

Ngày tải lên: 16/03/2014, 14:20

... as the answer key. For each Chinese and English word in the Wikipedia data, we first find whether there is a translation for the word in the extracted translation pairs. The Coverage of the ... For the examples in Table 2, the words频 道 (channel), 波 道 (wave passage), 信 道 (signal pas- sage), and 通道 (passage) are aligned with Channel, and the words间距(distance)...

Ngày tải lên: 17/03/2014, 02:20

... whether a candidate instance is a true class member is to see whether it can produce new candidate instances. If we instantiate our pattern with the candidate (i.e., “CLASS NAME such as CANDIDATE ... progresses. Initially, the seed is the only trusted class member and the only vertex in the graph. The bootstrapping process begins by instan- tiating the doubly-anchored pattern...

Ngày tải lên: 17/03/2014, 02:20

... distance between the assigned hypernym and the target word. The ideal distance is one, which would occur if the suggested ancestor is a parent. Grandparents are associated with distance two and ... recall and distance. Again, the conjunctive pattern outperformed the hypernym pattern. We as- sume that the frequency of the two patterns plays an important role (the frequency...

Ngày tải lên: 17/03/2014, 04:20

... of the protein and in particular the chemical resistance and binding capacity with respect to HNE. The incubation time of 20 min is the time required for the clearance of a molecule from the ... residues) compared with the porcine form (8.3%) [12], and because of their spatial arrange- ment in the 3D structure of the proteins (see below). The functionality of the...

Ngày tải lên: 23/03/2014, 10:20