Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... 3959 Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria Marı ´ aR.Go ´ mez-Garcı ´ a*, Manuel Losada and Aurelio Serrano Instituto ... families: family I, widespread in all types of organism [2], and family II, so far confined to a limited number of bacteria and archaea [3,4]. T...

Ngày tải lên: 16/03/2014, 11:20

... Naples, Italy 3 Institute of Biochemistry, University of Bayreuth, Germany 4 Istituto di Biostrutture e Bioimmagini, CNR, Naples, Italy 5 Istituto di Biochimica delle Proteine, CNR, Naples, Italy In ... Mose ´ Rossi 1,5 and Simonetta Bartolucci 1 1 Dipartimento di Biologia Strutturale e Funzionale, Universita ` degli Studi di Napoli Federico II, Naples, Italy 2 Istituto di Chimica Biomo...

Ngày tải lên: 18/02/2014, 18:20

... hST6Gal II and mST6Gal II. The two vertical dots indicate identical amino acid residues and single dots indicate similar amino-acid residues. The underlined amino acid residues indicate the sialylmotifs ... ST6Gal II (Eur. J. Biochem. 270) 953 rST3Gal III gene was excised with BamHI and HindIII and inserted at the BglII–HindIII site of the p119 transfer vector giving the p119-PS ST3...

Ngày tải lên: 08/03/2014, 08:20

... compilation ª 2007 FEBS stiff and tough, and protection from microbial degra- dation. This multifunctionality, together with the insoluble ⁄ sticky and complex nature of the adhesive, have hindered ... 54% identity and 65% similarity; Mrcp-19k versus Bicp-19k, 51% identity and 68% similarity; and Balcp-19k versus Bicp-19k, 61% identity and 75% similarity. All cp-19ks contain...

Ngày tải lên: 16/03/2014, 05:20

... b 1B subunit. The 5¢ and 3¢ untranslated regions are indicated by solid thin lines (b 1 ) andshadedthinlines(b 1B ), and the unidenti- fied 3¢ untranslated region of b 1B is indicated using the thin interrupted ... of the primary antibody with nonimmune serum, (b) omission of the primary antibody with the antibody dilution buffer (Zymed Laboratories Inc., San Francisco, CA, USA), and...

Ngày tải lên: 16/03/2014, 23:20

... organization of the oligomers originates from hexameric building blocks. Most of the interest in structure–function studies of Hcs is now focused on the importance of the oligomeric organization ... repulsive electrostatic interaction present in Panulirus is absent in Penaeus. Interestingly, all crustacean Hc, with the exception of Can. magister and Callinectes sapidus, exhibi...

Ngày tải lên: 23/03/2014, 13:20

... purification of functional hCYP11B1 expressed in E. coli. This will open new possibilities for analyzing this very important cytochrome P450 in vitro, including the detailed investigation of the ... be evaluated using the Michaelis–Menten equation (Fig. 7). Combined with the data obtained from Bia- core experiments, which indicated the possibility of more than one complex formation,...

Ngày tải lên: 30/03/2014, 04:20

... Animal Research Center, Nanjing University, China 2 School of Life Science, Nanjing Normal University, China 3 National Institute of Biological Science, Beijing, China Myosin light chain kinases ... transgenic mice exhib- ited similar expression patterns of recombinant 4Ig. Figure 3A shows a typical expression pattern in differ- ent lines, in which the level of expression of 4Ig var...

Ngày tải lên: 30/03/2014, 04:20

... Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus Hisashi Nishiwaki, Katsuhiko Ito, Katsuhiko Otsuki, Hiroyuki Yamamoto, Koichiro Komai and ... the insecticidal activity, suggesting that the rapid insect toxicity of sphingomyelinase C results from its phospholipid- degrading activity. Keywords: antlion; Bacillus cereus; inse...

Ngày tải lên: 30/03/2014, 13:20

... sodium channels with higher affinity than the ventricular myocyte sodium channels. Unlike Ts À c and CnII-10 (Ts À c is from Brazilian scorpion Tityus serrula- tus; CnII-10 is from Mexican scorpion ... D 600 ¼ 0.6, induction was initiated by the addition of 1.0 m M isopropyl thio-b- D -galactoside (IPTG). Cells were harvested 4 h after addition of IPTG by centrifugation and resuspe...

Ngày tải lên: 31/03/2014, 09:20