Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... attached at Lys9 of < /b> cET -1. Biotin was covalently attached to Cys1 of < /b> ET -1 and Lys-9, result- ing in bET -1. For coelution studies of < /b> ET -1 with ETB, Ligand binding of < /b> cell-free endothelin < /b> B receptor C. ... transmembrane segment 1 as an essential area for ET -1 binding and homodimer formation Christian Klammt 1 , Ankit...
Ngày tải lên: 16/03/2014, 10:20
Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... DEC1 (DEC1- DOrange) (lane 4), whereas no bands were detected by using basic region-deleted D EC1 ( DEC1Dbasic and DEC1:4– 232Dbasic) (lanes 2 and 14 ) or bHLH domain-deleted DEC1 (DEC1DbHLH and ... the b inding activity (lanes 9, 10 and 13 ), whereas deletion of < /b> 296 residues (VP16-DEC1:4 11 6) decreas ed the binding (lane 11 ), and deletion of < /b> 312 residues (VP...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... - 31 - 20 - FnBPB -1 FnBPB-2/3 FnBPB-4 FnBPB-5 FnBPB-6 FnBPB-7 FnBPB-8 FnBPB-9 FnBPB -10 FnBPB -11 GST FnBRB FnBPB -1 FnBPB-2/3 FnBPB-4 FnBPB-5 FnBPB-6 FnBPB-7 FnBPB-8 FnBPB-9 FnBPB -10 FnBPB -11 GST FnBRA FnBPA -1 FnBPA-2 FnBPA-3 FnBPA-4 FnBPA-5 FnBPA-6 FnBPA-7 FnBPA-8 FnBPA-9 FnBPA -10 FnBPA -11 GST FnBRA A 490 ... - 31 - 20 - kDa 97 - 3.0 2.5 2.0 1. 5 1. 0 0.5 0.0 66 - 31...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
... supernatant was resolved by SDS ⁄ PAGE and myc–GALE and EGFP pro- teins were detected by western analysis < /b> using antibody against myc (9E10; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and antibody ... co-transfected and analyzed as in (A). (C) FLAG–GALE was trans- fected into stable ldlD lines expressing myc-GALE as indicated and immunoprecipitation was performed and...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
... mammalian meva- lonate kinase and ATP. Insights into the reaction mechanism and human < /b> inherited disease. J. Biol. Chem. 277, 18 134 18 142. 10 . Romanowski, M.J., Bonanno, J .B. & Burley, S.K. (2002) ... Consortium: an integrated molecular analysis < /b> of < /b> genomes and their expression. Genomics 33, 15 1 15 2. 25. Bradford, M.M. (19 76) A rapid and sensitive...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... Kra ´ lovopolska ´ 13 5, CZ- 612 65 Brno, Czech Republic Fax: +420 5 41 517 18 4 Tel: +420 5 41 211 293 E-mail: brzoboha@ibp.cz (Received 11 July 2008, revised 17 September 2008, accepted 9 October 2008) doi :10 .11 11/ j .17 42-4658.2008.06735.x b- Glucosidases ... b- glucosid- ases from 13 plant species. Fig. S2. Purity of < /b> the wild-type and mutant Zm-p60 .1 b- glucosidas...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt
... work was started, a probe was obtained by PCR using oligonucleotides FRBGL1 and FRBGL2 or FRBGL1 and FRBGL3, based on the con- served sequences of < /b> dtxR homologous genes [1] , and the DNA of < /b> S. ... analyzed by using the mascot soft- ware [29]. Immunodetection analysis < /b> of < /b> DmdR1 and DmdR2 Western blot analysis < /b> of < /b> DmdR1 and DmdR2, after SDS ⁄ PA...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... drincovich@cefobi-conicet.gov.ar (Received 15 January 2 010 , revised 11 February 2 010 , accepted 12 February 2 010 ) doi :10 .11 11/ j .17 42-4658.2 010 .07 617 .x Escherichia coli phosphotransacetylase (Pta) catalyzes the reversible inter- conversion ... (2 010 ) 19 57 19 66 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 19 61 Oligomeric state of < /b> Pta and the truncated...
Ngày tải lên: 06/03/2014, 11:20
Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... regulation of < /b> the IKK complex by HSP70 and HSP90. Immunol Lett 11 7, 9 15 . 18 Brand IA & Heinickel A (19 91) Key enzymes of < /b> carbo- hydrate metabolism as targets of < /b> the 11 .5-kDa Zn(2+)- binding ... ª 2 010 FEBS 31 Andersen SS (2000) Spindle assembly and the art of < /b> regulating microtubule dynamics by MAPs and Stath- min ⁄ Op18. Trends Cell Biol 1...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... c 2 ⁄ c 1 2,4-Pentanedione 1 1 1, 1-Difluoro-2,4-pentanedione 2 · 10 )3 8.2 1, 1 ,1- Trifluoro-2,4-pentanedione 3 · 10 )4 NM 1- Phenyl -1, 3-butanedione 5 · 10 )2 0.3 4,4-Difluoro -1- phenyl -1, 3-butanedione ... of < /b> Burkholderia xenovorans LB400. Table S3. Growth and formation of < /b> oxygenase activity by Burkholderia xenovorans LB400. Table S4. Product distribution f...
Ngày tải lên: 18/02/2014, 06:20