Báo cáo khoa học: Mammalian 105 kDa heat shock family proteins suppress hydrogen peroxide-induced apoptosis through a p38 MAPK-dependent mitochondrial pathway in HeLa cells potx

... caspase-3 activation, as they do not suppress the activation of caspase-3 mediated by heat shock. In mammalian cells, one of the main pathways that activates procaspase-3 is via mitochondria. ... pathway. Results Hsp10 5a and Hsp105 b suppress apoptosis induced by H 2 O 2 but not by heat shock in HeLa cells Previously, we have established human HeLa cell lines (H...

Ngày tải lên: 16/03/2014, 06:20

... retardation and exagger- ated production of the pro -in ammatory cytokine tumour necrosis factor -a, without affecting basal HSP expression [38]. Although HSF1 gains DNA-binding and transactivating abilities ... transcriptional activators because it can bypass a need for critical general transcription factors and co-activators, including general transcription fac- tor IIA (TFIIA) [31], Kin2...

Ngày tải lên: 18/02/2014, 04:20

... (forward) GAGATCATCAAGCCCACCACAAC 112 Hsp40 (reverse) CGGGAAACTTAATGTCGAAGGAGAC Hsp60 (forward) ACATCTCGCCGTACTTCATCAACTC 66 Hsp60 (reverse) GGAGGAGGGCATCTTGGAACTC Hsp67Ba (forward) TGGATGAACCCACACCCAATC ... TGGATGAACCCACACCCAATC 89 Hsp67Ba (reverse) CGAGGCAACGGGCACTTC Hsp68 (forward) GAAGGCACTCAAGGACGCTAAAATG 88 Hsp68 (reverse) CTGAACCTTGGGAATACGAGTG Hsp70Aa (forward) TCGATGGTACTGACCAAGATGAA...

Ngày tải lên: 06/03/2014, 09:22

... shock transcription factor family Noritaka Yamamoto 1 , Yukiko Takemori 1 , Mayumi Sakurai 2 , Kazuhisa Sugiyama 2 and Hiroshi Sakurai 1 1 Department of Clinical Laboratory Science, Kanazawa University ... generation, Keywords crystallin; heat shock element; heat shock protein; heat shock response; heat shock transcription factor Correspondence H. Sakurai, Department of Cl...

Ngày tải lên: 07/03/2014, 00:20

... repeats from human tenascin studied through a sequence frame-shift approach Francesco Zanuttin, Corrado Guarnaccia, Alessandro Pintar and Sa ´ ndor Pongor International Centre for Genetic Engineering ... Hoffman, S., Cunningham, B .A. & Edelman, G.M. (1989) A detailed structural m odel of cytotact in: protein homo- logies, alternative RNA splicing, and binding regions. Proc. Natl Ac...

Ngày tải lên: 23/03/2014, 13:20

... by PCR with Taq DNA polymerase (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The cDNA was subcloned into the EcoRV ... findings indicate that both palmitoylated and nonpalmitoylated mammalian Hh proteins can act as signaling molecules. It is nota- ble that while cholesterylation of Hh protein is an intramolecu...

Ngày tải lên: 18/02/2014, 16:20

... from sulfonoquinovosyl diacylglycerols of sea urchin. Trans- plantation 74, 261–267. 3 Mizushina Y, Watanabe I, Ohta K, Takemura M, Sahara H, Takahashi N, Gasa S, Sugawara F, Matsuk- age A, Yoshida S & ... transcriptase type 1 from a marine red alga, Gigartina tenella. Chem Pharm Bull (Tokyo) 46, 684– 686. 5 Ohta K, Mizushina Y, Hirata N, Takemura M, Sugaw- ara F, Matsukage A, Yoshida...

Ngày tải lên: 19/02/2014, 17:20

... cofactors are located in the C-terminal 85 kDa, N-terminal 20 kDa and intermediate 40 kDa domains, respectively [24,35,36]. The oxidation of xan- thine takes place at the molybdenum center, and ... of hypoxanthine to xanthine or xanthine to uric acid in the metabolic pathway of purine degradation in higher animals [1,2]. In humans, the enzyme is the target of therapeutic drugs...

Ngày tải lên: 16/03/2014, 06:20

... pPICZaA 6 ATATAT GCGGCCGCCTAGTGATGGTGATGGTGATGGAGCCCCAGGTATTCAGCCAG 7 ATAT GAATTCCATCACCATCACCATCACTTCTACACCATTTGGAGCGGC Cloning of isoQC (F48) N-His into pPICZaA 8 ATATAT GCGGCCGCCTAGAGCCCCAGGTATTCAGC 9 ... ATATAT GCGGCCGCCTAGAGCCCCAGGTATTCAGC 9 ATAT GAATTCCATCACCATCACCATCACGAGGAGCTGCCGCTGGGCCG Cloning of isoQC (E60) N-His 10 ATAT GAATTCGAGGAGATGTCACGGAGC Cloning of murine isoQC (E61) into p...

Ngày tải lên: 23/03/2014, 04:20

... 5445 Frequent inactivating mutations of caspase-8 have been reported in advanced colorectal carcinomas [183], hepatocellular carcinomas [184], gastric carcinomas [185] and in a single head and neck carcinoma ... domains (DEDs) in their prodomains, and are also apoptotic family members. Caspases-3, -6, -7 and -14 have short prodomains and are effector caspases in the apoptotic pat...

Ngày tải lên: 23/03/2014, 15:21