Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot

... FEBS Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental ... full- length cDNA of PO -CHH and identified the presence of the neuropeptide forms in the ES and PO. For th...

Ngày tải lên: 16/03/2014, 06:20

... phosphorylation to the corresponding triphosphate nucleoside, converting the pro-drug to the active cytotoxic drug. The nucleo- side analogue can be incorporated into the DNA chain and cause chain termination, ... hydro- gen bonds with the base, was mutated to N in order to see how the increased distance between the base and substrate-binding amino acid affected...

Ngày tải lên: 19/02/2014, 02:20

... regulatory TnC-binding site, from actin, resulting in the release of the inhibition and muscle contraction [17]. The structural TnC-bind- ing site interacts with the C-domain of TnC in both the ... TnC- binding site and the C-domain of TnC accompanying Ca 2+ binding to site IV of TnC. In vertebrate tropo- nin, the activation may be a result of the inter...

Ngày tải lên: 20/02/2014, 01:20

... sulfonamide [R–S(O 2 )–NH– R¢] [23], sulfamate [R–O–S(O 2 )–NH–R¢] [24], sulfamide [R–NH–S(O 2 )–NH–R¢] [25,26], and N -acylsulfamate [R–O–S(O 2 )–NH–C(O)–R¢] [27]. Of these functional groups, only the ... the K m values is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initial binding of both substrates. In UpG, the bindi...

Ngày tải lên: 14/02/2014, 22:20

... that has the IMPACT tag fused to the authentic C-terminal Gly145. Expression and purification Recombinant protein was produced by cultivating the expression strain in shaking flasks containing LB ... by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by superoxide in the course of t...

Ngày tải lên: 18/02/2014, 06:20

... seems to have on the RNase activity of the enzymes may be linked to an increase in Na + around the phosphate groups and the 2¢-OH, which reduces the negative charge, and hence the affinity of the enzyme ... N-terminus (Thr and Met) are encoded by the expression vector. An analysis of the amino-acid composition shows that VsEndA contains 13 more lysines an...

Ngày tải lên: 19/02/2014, 05:20

... DEC1 with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for ... 5 ). These r esults indicate that the bHLH region, including His57 and Arg65, is responsible for t he E-box binding. Determination of the region in BMAL1 for bi...

Ngày tải lên: 19/02/2014, 16:20

... Ponting CP (1998) Evidence that the conformation of unliganded human plasminogen is maintained via an intramolecular interaction between the lysine-binding site of kringle 5 and the N-terminal peptide. ... [35] and have used them to analyse, in functional isolation, the role of these LBS on plas- min(ogen) binding and activation. The effect of these mAbs on plasm...

Ngày tải lên: 20/02/2014, 01:20

... destabilize the proteins leading to spurious protein–protein or /and protein–DNA interactions. The crystal structure of Ydc2 shows that deletion of the two N-terminal helices 1 and 2 would expose to the ... base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal structure...

Ngày tải lên: 20/02/2014, 11:20

... preventing the release of EF-Tu–GDP from the ribosome [24]. These antibiotics bind at the interface between domain 1 (guanine nucleotide-binding domain, G-domain) and domain 3 of EF-Tu, and lock EF-Tu–GDP ... translation indirectly by inhibition of mRNA synthesis during amino acid starvation [2 0–2 3]. Kirromycin is one in a family of antibiotics that inhibits protein...

Ngày tải lên: 21/02/2014, 00:20