Báo cáo khoa học: Small heat shock protein Hsp27 prevents heat-induced aggregation of F-actin by forming soluble complexes with denatured actin docx

... Authors Journal compilation ª 2007 FEBS Small heat shock protein Hsp27 prevents heat- induced aggregation of F -actin by forming soluble complexes with denatured actin Anastasia V. Pivovarova 1,2 , ... stable and highly soluble complexes with denatured actin, and this is the mecha- nism by which sHSPs prevent the aggregation of F -actin. Sta...

Ngày tải lên: 16/03/2014, 06:20

... [21]. Quantitation of proteins in dog pancreas microsomes The amount of protein present in the band of a gel of a GST hybrid preparation was determined by comparison with protein standards that ... 6A,B). In the absence of ATP, a proportion of BiP cofractionat- ed with Grp170, with an elution maximum of both proteins at a position that corresponded to a molecular mas...

Ngày tải lên: 30/03/2014, 03:20

... 2010) doi:10.1111/j.1742-4658.2010.07827.x The heat shock response was originally characterized as the induction of a set of major heat shock proteins encoded by heat shock genes. Because heat shock proteins act as molecular ... eukaryotes, the heat shock response is regulated mainly at the level of transcription by heat shock factors (HSFs) [3]. Heat shoc...

Ngày tải lên: 18/02/2014, 04:20

... activated by members of the Ras family of small G proteins. The activity of Ras proteins is under tight control of several classes of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins. ... prior to 5 min of treatment with 10 lm serotonin (Fig. 6B). Increasing the free intracel- lular levels of Ca 2+ by treatment of HEK293 cells with thapsigarg...

Ngày tải lên: 19/02/2014, 18:20

... family of proteins that bind DNA at unmethylated CpG islands [7], which are always located to active chroma- tin. Other proteins identified are fungal homologues, of which suppressor of PRP protein ... term SPP1. All these proteins contain a small, previously undetected domain downstream of the PHD, which we called dPHD, that allows connection of these famil- ies (despite the sma...

Ngày tải lên: 07/03/2014, 21:20

... Chi 1,2 1 Institute of Protein Research, Tongji University, Shanghai, China 2 Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China Introduction Proteinase inhibitors ... fragments of MBTI were successfully separated by restricted peptic digestion [23], and the sequence of MBTI was eluci- dated [24]. The active fragment with Arg at the reac-...

Ngày tải lên: 15/03/2014, 09:20

... overexpression of FXR1 suppresses LPS- induced TNF-a protein production. Targeting the p38 mitogen-activated protein kinase pathway of LPS-treated cells with small molecule inhibitors can induce FXR1 protein ... obtained by measure- ment of the TNF-a protein concentration by ELISA (Fig. S1A–C). To determine whether TGF-b1-dependent inhibition of LPS-induced TNF-a prot...

Ngày tải lên: 22/03/2014, 21:21

... to permit quantification of protein degradation at protein concentrations of < 20 lgÆmL )1 , autoproteolysis at lower protein concentrations was followed by measure- ment of the remaining activity ... Autoproteolysis was quantified by evaluation of the bands of intact protein in SDS ⁄ PAGE gels after staining with Coomassie brilli- ant blue. Figure 2 shows the autopro...

Ngày tải lên: 19/02/2014, 17:20

... phosphorylation by Akt (Eur. J. Biochem. 271) 3335 Phosphorylation of dHAND did not affect the dimerization activity with E47 To clarify the mechanisms of the inhibition of dHAND activity by Akt phosphorylation, ... activity of dHAND could be affected by Akt phosphorylation. The influence of phos- phorylation by Akt on the DNA binding activity was examined by gel shift an...

Ngày tải lên: 07/03/2014, 16:20

... (2002) Structure and properties of the small heat shock pro- teins (sHsp) and their interaction with cytoskeleton proteins. Biochemistry (Mosc) 67 , 511–519. 5 Macario AJL (1995) Heat- shock proteins and molecular chaperones: ... interaction of a-crystallin with unfolded proteins. Biochim Biophys Acta 1252, 251–260. 23 Bova MP, Mchaourab HS, Han Y & Fung BK-K (2000) Subun...

Ngày tải lên: 07/03/2014, 04:20