Báo cáo khoa học: Identification and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx
Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... expression of T-bet has beenseen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... CD4+CD25+Foxp3+Tregor whetherthey induce the production of these cytokines from another population of cells [20].To date, our knowledge about the different types of Th and Tregresponses relates ... end of the gene, where the zebrafish and human sequences appearto be quite diverse. Together with the well-conservedgene organization, there is a conservation of syntenybetween the human and...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... reporter); the duration of the development of blue color in the LacZ filter-lift assay (activation of LacZ reporter); and the b-gal units calculated from the liquid LacZ assay (activation of LacZ ... of cellular media-tors called receptor-regulated Smads (R-Smads). The R-Smads of the TGF b–activin pathway include Smad2 and Smad3. The R-Smads of the BMP pathwayinclude Smads 1, 5 and 8. The ... phos-phorylates the R-Smad at its C-terminal MH2 domain,causing the R-Smad to dissociate from the receptor. The phosphorylation ⁄ activation of the R-Smad allowsit to interact with another component of the...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... acids at the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the DBD of SmNR1 (Ile247-Ser372) and SmRXR1 (Glu251-Asn376)were tested in vitro. Lanes 1, 5, 9, 13, 17, 21, 25 and ... most NRs, and the length of the hinge in SmNR1 thus can beestimated to be 140 amino acids. The role of the large hinge in schistosome NRs remains unknown. The degree of conservation of the LBD ... 6A). The results sugges-ted that both full-length and the A ⁄ B domain of SmNR1 activated transcription of GAL4 reporter genein the absence of ligand, while the C-F domain didnot. Thus the...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... periods of time(Fig. 3). As shown, the addition of stressors to the cul-tures inhibits growth without killing the cells. The hybridizing band in the northern analysisshowed the expected size of ... cells and purified from the soluble fraction of bacterial cells as described in Experimental proce-dures. The yield of C49S was the same of thatobtained for the wild-type protein. The activity of ... other microrganisms [24]. Moreover, the basal level of bcp2 transcript in the early and mid-exponential, and the stationary phase of growthsuggests that bcp2 is not involved in the control of endogenous...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... analysis of the structure of the latter made it possible to improve the currentknowledge of the mammalian PR48 cDNA. To extend thesefindings, the genomic structures of the human PR130 and PR48 ... laevisoocytes are often used as a model system to study specificaspects of the cell cycle. Oocytes taken from the ovary of the frog are arrested in prophase of the first meiotic division.Addition of progesterone ... different classes of cDNAs were isolated and analysed. The first is the Xenopus orthologue of PR130, the second proved to be a novel splice variant and the third is the Xenopus orthologue of PR48. Moreover,...
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Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
... composed of two each of the a and b subunits.Analysis of the N-terminal transit peptide of CLGATAnalysis of the CLGAT cDNA predicted a chloroplasttransit peptide upstream from the N-terminus of CLGAT. ... clone of the GAT, gene-specific primers were designed from the sequence of watermelon EST AI563351 and used for 5¢- and 3¢-RACE. The cloned cDNA encoded a proteincomposed of 460 amino acids. The ... examine whether the precursor of CLGAT is imported into the chloroplasts, we prepareda plasmid in which the first 26 codons of the CLGATABFig. 3. Molecular mass of GAT purified from leaves of wild...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... constit-uents of the cell surface. They are found at the outermostends of the sugar chain of animal glycoconjugate. They actas an important component of the ligands recognized by the lectins. Recognition ... microtiter well and mixed. The values of the HAI titerwere determined after 60 min of incubation and expressed as the highest dilution of sugars that inhibited the agglutination of erythro-cytes. ... °C. The eluant wascollected at a rate of 0.6 mLÆmin)1.Thecolumnwaswashed with HSB until the A280 of the effluent was < 0.002. The column was further washed with LSB at 4 °C until the A280of...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... phosphatase. Of potential interest in such a context was the finding that the rate of isomerization of the histidine-proline bond in the peptide Suc-Ala-His-Pro-Phe-pNA isinfluenced by the degree of protonation ... were from Amersham Biosciences.Preparation of the phosphohistidine-containing peptideSuc-Ala-His(P)-Pro-Phe-pNA The protocol used was adapted from that used for the synthesis and isolation of ... gradient of MDEA from 0.085Mto0.765Min 20% methanol at 1 mLÆmin)1. The absorbanceat 315 nm was monitored and the degree of dephosphory-lation was calculated from the peak areas of phosphorylatedand...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... cloned into the vector pQE16 in frame with the His-tag-encodingsequence. The lepB gene was PCR ampli®ed from E. coliDNA using the primers Lep1 and Lep2 (Table 2), and thencloned into the pQE16 ... ABLASTsearch of the nucleotidesequence of the ampli®ed DNA fragment revealed the presence of three ORFs, the deduced proteins having 70, 77, and 67% identity to proteins encoded by the yhjE-sipV-yhjGTable ... extension, the forwardprimers CH1 and C H2 were designed for conservedregions HFGAGNIG and HGSAPDIAG of the genesmtlD and ycsA, which map in B. subtilis upstream of the searched sipU, and used...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... enzyme complex from A. fulgidus (Eur. J. Biochem. 269) 1897 Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides ... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. ful-gidus enzyme is still unknown. Therefore, no substrate couldbe added to the titration ... Features of the subunits of the Hme complex from A. fulgidus. Data are eith er derived from the analysis of the sequence (calculatedmolecular mass, predicted transmembrane helices, cofactor binding...
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