Báo cáo khoa học: Identification and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx
Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... expression of T-bet has been seen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... CD4 + CD25 + Foxp3 + T reg or whether they induce the production of these cytokines from another population of cells [20]. To date, our knowledge about the differen...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... reporter); the duration of the development of blue color in the LacZ filter-lift assay (activation of LacZ reporter); and the b-gal units calculated from the liquid LacZ assay (activation of LacZ ... of cellular media- tors called receptor-regulated Smads (R-Smads). The R-Smads of the TGF b–activin pathway include Smad2 and Smad3. The R-Smads of the BMP pa...
Ngày tải lên: 18/02/2014, 16:20
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... acids at the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the DBD of SmNR1 (Ile247-Ser372) and SmRXR1 (Glu251-Asn376) were tested in vitro. Lanes 1, 5, 9, 13, 17, 21, 25 and ... most NRs, and the length of the hinge in SmNR1 thus can be estimated to be 140 amino acids. The role of the large hinge in schistosome NRs remains unknown....
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... periods of time (Fig. 3). As shown, the addition of stressors to the cul- tures inhibits growth without killing the cells. The hybridizing band in the northern analysis showed the expected size of ... cells and purified from the soluble fraction of bacterial cells as described in Experimental proce- dures. The yield of C49S was the same of that obtained for t...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... analysis of the structure of the latter made it possible to improve the current knowledge of the mammalian PR48 cDNA. To extend these findings, the genomic structures of the human PR130 and PR48 ... laevis oocytes are often used as a model system to study specific aspects of the cell cycle. Oocytes taken from the ovary of the frog are arrested in prophase of...
Ngày tải lên: 08/03/2014, 08:20
Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
... composed of two each of the a and b subunits. Analysis of the N-terminal transit peptide of CLGAT Analysis of the CLGAT cDNA predicted a chloroplast transit peptide upstream from the N-terminus of CLGAT. ... clone of the GAT, gene- specific primers were designed from the sequence of watermelon EST AI563351 and used for 5¢- and 3¢-RACE. The cloned cDNA en...
Ngày tải lên: 20/02/2014, 03:20
Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... constit- uents of the cell surface. They are found at the outermost ends of the sugar chain of animal glycoconjugate. They act as an important component of the ligands recognized by the lectins. Recognition ... microtiter well and mixed. The values of the HAI titer were determined after 60 min of incubation and expressed as the highest dilution of sugars that...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... phosphatase. Of potential interest in such a context was the finding that the rate of isomerization of the histidine- proline bond in the peptide Suc-Ala-His-Pro-Phe-pNA is influenced by the degree of protonation ... were from Amersham Biosciences. Preparation of the phosphohistidine-containing peptide Suc-Ala-His(P)-Pro-Phe-pNA The protocol used was adapted from tha...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... cloned into the vector pQE16 in frame with the His-tag-encoding sequence. The lepB gene was PCR ampli®ed from E. coli DNA using the primers Lep1 and Lep2 (Table 2), and then cloned into the pQE16 ... A BLAST search of the nucleotide sequence of the ampli®ed DNA fragment revealed the presence of three ORFs, the deduced proteins having 70, 77, and 67% identity to...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... enzyme complex from A. fulgidus (Eur. J. Biochem. 269) 1897 Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides ... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. ful- gidus enzyme is still unknown. Therefore...
Ngày tải lên: 21/02/2014, 03:20