... define the core of the protein’s Nstate [21]. For the T domain, these were the center of TH5 and TH8, and the N-terminal half of TH9. Alla-helices (TH1, 3, 4, 5’, 6, 7 and the remaining parts of TH5 ... in the N state and the evolution of the overallstructure of the protein in the MG state. This degree of resolution is unprecedented for the T domain of the diphtheria toxin. Three levels of ... In the case of the T domain, the MGstate corresponds to the functional state, which initi-ates the translocation of the catalytic domain. Here, the data allowed identification of the core of the...