Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc

... complex gangliosides are apically sorted in polarized MDCK cells, and that GD3 and GM1 gangliosides are internalized by clathrin-indepen-dent endocytosis to follow different intracellular destinations.AbbreviationsCHO, ... participate in theendocytic process of GD3 in MDCK cells. ABCFig. 5. GD3 and GM1-CTx are internalized in MDCK cells by clathrin-independent endocytosis. (A) GD3-expressing MDCK cells were ... FEBS Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis Pilar M. Crespo, Natalia von Muhlinen, Ramiro Iglesias-Bartolome´and...

Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

... (B), the side-chains of residues 29, T34 and E131 are shown. The hydrogen bonds betweenN29 and Oc and T34 and Oc, and between N29 and Nd and E131 and Oe2, in the wild-type protein are shown as green ... the side-chains of Y24, residue 39, F41 and Q149 are shown. The hydrogen bondsbetween D39 and Od1 and Q149 and Ne, and between D39 and Od2 and Q149 and Ne, in the wild-type protein are shown as ... wild-typeprotein by 5.4 °CinTm and 3.9 kJÆmol)1 in DDGm.D136H-RNase HI is more stable than the wild-typeprotein by 9.7 °CinTm and 7.0 kJÆmol)1 in DDGm.The stabilities of the mutant proteins againsturea-induced...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... displaying a high-afﬁnity binding site for bilirubin. It is competitively inhibited by grapeanthocyanins, including aglycones and their mono- and di-glycosylatedderivatives. In plant cells, ... to rat and human ligandins: characteri-zation of a binding-site peptide. Biochim Biophys Acta955, 296–300.52 Gregus Z & Klaassen CD (1982) Role of ligandin as abinding protein and as ... seconddecrease in absorption was brought about by valinomycin-induced K+diffusion potential by adding 5 l g valinomycin(Fluka) in 1 lL methanol. Such K+diffusion drove thesubstrate into the...

Báo cáo khoa học: Context-dependent effects of proline residues on the stability and folding pathway of ubiquitin docx

... which points tothe stabilizing effect of this mutation not being realized in the folding TSE, indicative of the loop between theN-terminal b-hairpin and the main a-helix remainingﬂexible in the ... A.R. (1991) Protein engineering in analysis of protein folding pathways and stability. MethodsEnzymol. 202, 8 2–112.Ó FEBS 2004 Proline residues in ubiquitin stability and folding (Eur. J. Biochem. ... 2004protein stability, and their involvement in the foldingpathway from studies of refolding/unfolding kinetics. Whilethe single point mutation P37A is destabilizing b y4.5 kJÆmol)1, in contrast...

Báo cáo khoa học: High levels of structural disorder in scaffold proteins as exempliﬁed by a novel neuronal protein, CASK-interactive protein1 pot

... thatcan interact with signalling proteins containing SH2domains. Insulin receptor substrate 1, for example, con-tains an N-terminal Pleckstrin homology domain and aphosphotyrosine-binding domain, ... regions are oftendirectly involved in protein–protein interactions [42],these points help us to interpret the possible role ofdisorder in Caskin1 and in other scaffold proteins. Toobtain a balanced ... proline-rich domain (PRD) in this work. Caskin1 can bind the Cask adaptor protein[1], Abl-interactor-2 (Abi2), and another nine proteinsshown in this work, and is presumably involved in the signal...

Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

... (patatin, found in potatotubers) [28] and iPLA2b [23] – iPLA2c contains a consensussite for nucleotide binding and a lipase consensus motif in itsC-terminal half [26]. Although the intracellular ... mechanismsmodulating iPLA2c expression and processing in differentcell types, we examined multiple iPLA2c constructs in different cellular contexts and in intact rat myocardium.Herein, we demonstrate ... System (Invitrogen) according t o the manufac-turer’s instructions. Sf9 cells were grown and infected forpreparation of recombinant protein extracts as previouslydescribed [26]. In brief, Sf9 cells...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... extension to the protein kinase folds backon the kinase domain and interferes with the substratebinding sites. In the case of the titin and twitchinkinases, the autoinhibitory sequence acts ... proteinkinases including myosin light chain kinase (MLCK),CaM kinases I, II and IV (CaMKI, CaMKII and CaMKIV, respectively), CaM kinase kinase (CaMKK),titin kinase, and death associated kinase [4]. Structuralstudies ... complex consisting of four copies of foursubunits, a, b, c and d. The a and b subunits are regu-latory and are the sites of phosphorylation and meta-bolite binding and are also regulated by...

Tài liệu Báo cáo khoa học: Type I antifreeze proteins expressed in snailﬁsh skin are identical to their plasma counterparts doc

... solutionfreezing point noncolligatively by binding to certainsurfaces of ice crystals, modifying their structure and inhibiting further growth. The difference between thelowered freezing point and unaltered ... expression only in skin tissue. Taken together, the evidence indicates thatthe circulating plasma AFPs and skin localized AFPs are identical proteins that are normally expressed by the same skin-speciﬁc ... to each other and their skin transcripts produce proteins that are identi-cal to their corresponding plasma proteins.ResultscDNA library screening and cloning of snailﬁshskin AFPA cDNA library...

Tài liệu Báo cáo khoa học: "Which words are hard to recognize? Prosodic, lexical, and disﬂuency factors that increase ASR error rates" ppt

... (Fosler-Lussier and Morgan, 1999; Shinozaki and Furui, 2001) and thatfast speech increases error rates (Siegler and Stern,1995; Fosler-Lussier and Morgan, 1999; Shinozaki and Furui, 2001). Siegler and ... withShinozaki and Furui (2001) and Siegler and Stern(1995). The effects of pitch minimum and maximum are not shown for reasons of space, but are similarto pitch mean. Also not shown are intensity ... features in Figure2. We did not include minimum and maximum val-ues for pitch and intensity because they are highlycorrelated with the mean values, making parameterestimation in the combined...

Báo cáo khoa học: Many fructosamine 3-kinase homologues in bacteria are ribulosamine⁄erythrulosamine 3-kinases potentially involved in protein deglycation docx

... Xyluloselysine was synthesized as described forribuloselysine and fructoselysine was synthesized aspreviously described [2]. Pentitollysine was prepared by incubating (in 0.1 mL) 50 mm ribuloselysine ... recombinant fructosamine 3-kinase (FN3K) and low-molecular-weight protein-tyrosine-phosphatase (LMW-PTP) homologues. Restriction sites are written in italics. ATG and stop codons are underlined. ... aureus and Thermus thermophilus) were overexpressed in E. coli, puriﬁed and their kinetic properties investigated. Four were ribu-losamine ⁄ erythrulosamine 3-kinases acting best on free lysine and...

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